Characterization Of Malaysian Isolates Of Bovine Herpes Virus I Of Buffalo Origin

This research was carried out to investigate two isolates of bovine herpes virus I (BHV -l) isolated from buffaloes at Universiti Pertanian Malaysia , Serdang , Selangor Darul Ehsan, and compare them to a reference cattle strain of BHV- l ( Cooper strain) . The cytopathogenic and serologic prop...

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Main Author: Cheow, Loretta Marie
Format: Thesis
Language:English
English
Published: 1992
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Online Access:http://psasir.upm.edu.my/id/eprint/12299/1/FPV_1992_3_A.pdf
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spelling my-upm-ir.122992011-07-13T04:17:27Z Characterization Of Malaysian Isolates Of Bovine Herpes Virus I Of Buffalo Origin 1992-04 Cheow, Loretta Marie This research was carried out to investigate two isolates of bovine herpes virus I (BHV -l) isolated from buffaloes at Universiti Pertanian Malaysia , Serdang , Selangor Darul Ehsan, and compare them to a reference cattle strain of BHV- l ( Cooper strain) . The cytopathogenic and serologic properties of the three virus isolated (Buffalo respiratory , Buffalo genital , and the Cooper strain) were first studied. The cytopathogenicity of the three BHV-1 isolates was investigated using Acridine Orange staining (to compare localization of DNA) , Hematoxylin and Eosin staining (to compare morphologic changes induced in cell culture), the indirect Immunoperoxidase ( lIP) staining method ( to compare localization o f the virus antigen ) , plaque assay, and Negative Contrast Electron Microscopy. 1992-04 Thesis http://psasir.upm.edu.my/id/eprint/12299/ http://psasir.upm.edu.my/id/eprint/12299/1/FPV_1992_3_A.pdf application/pdf en public masters Universiti Pertanian Malaysia Faculty of Veterinary medicine English
institution Universiti Putra Malaysia
collection PSAS Institutional Repository
language English
English
topic


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Cheow, Loretta Marie
Characterization Of Malaysian Isolates Of Bovine Herpes Virus I Of Buffalo Origin
description This research was carried out to investigate two isolates of bovine herpes virus I (BHV -l) isolated from buffaloes at Universiti Pertanian Malaysia , Serdang , Selangor Darul Ehsan, and compare them to a reference cattle strain of BHV- l ( Cooper strain) . The cytopathogenic and serologic properties of the three virus isolated (Buffalo respiratory , Buffalo genital , and the Cooper strain) were first studied. The cytopathogenicity of the three BHV-1 isolates was investigated using Acridine Orange staining (to compare localization of DNA) , Hematoxylin and Eosin staining (to compare morphologic changes induced in cell culture), the indirect Immunoperoxidase ( lIP) staining method ( to compare localization o f the virus antigen ) , plaque assay, and Negative Contrast Electron Microscopy.
format Thesis
qualification_level Master's degree
author Cheow, Loretta Marie
author_facet Cheow, Loretta Marie
author_sort Cheow, Loretta Marie
title Characterization Of Malaysian Isolates Of Bovine Herpes Virus I Of Buffalo Origin
title_short Characterization Of Malaysian Isolates Of Bovine Herpes Virus I Of Buffalo Origin
title_full Characterization Of Malaysian Isolates Of Bovine Herpes Virus I Of Buffalo Origin
title_fullStr Characterization Of Malaysian Isolates Of Bovine Herpes Virus I Of Buffalo Origin
title_full_unstemmed Characterization Of Malaysian Isolates Of Bovine Herpes Virus I Of Buffalo Origin
title_sort characterization of malaysian isolates of bovine herpes virus i of buffalo origin
granting_institution Universiti Pertanian Malaysia
granting_department Faculty of Veterinary medicine
publishDate 1992
url http://psasir.upm.edu.my/id/eprint/12299/1/FPV_1992_3_A.pdf
_version_ 1747811345557880832