Extract Of Gelidiella Acerosa (S-Act-1) As A Capacitation Agent For In Vitro Fertilisation In Goat
A study was conducted to determine if goat ovaries obtained from a slaughterhouse could be used for in vitro fertilisation (lVF). Three experiments were conducted to ascertain if a substance named S-ACT-l, extracted from a marine algae, (Gelidiella acerosa), with a chemical structure similar to h...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English English |
| Published: |
1997
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/12327/1/FPV_1997_6_A.pdf |
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| Summary: | A study was conducted to determine if goat ovaries obtained from a
slaughterhouse could be used for in vitro fertilisation (lVF). Three experiments were
conducted to ascertain if a substance named S-ACT-l, extracted from a marine algae,
(Gelidiella acerosa), with a chemical structure similar to heparin could be used for
sperm capacitation.
The acrosome reaction was tested by a dual staining technique (Experiment 1 )
and the zona free hamster penetration test (Experiment 2). In vitro maturation o f goat
oocytes and fertilisation were assessed by the number of zygotes cleaving beyond the
2-cell stage (Experiment 3).
Freshly ejaculated semen from two fertile bucks were used for sperm
capacitation using two defined media (DM-H and DM-Ca). Heparin and S-ACT-l
were tested at concentrations of 10, 20, 50 and 100 ug/ml. After incubating for 15
min. at 39° C with 5 % CO2 in air under maximum humidity, samples were stained
with trypan blue and Giemsa stain (dual stain). The results indicated that the sperm
treated with heparin and S-ACT - 1 showed similar staining characteristics. |
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