Bacterial cellulose production from extracted date syrup

Bacterial cellulose (BC) is used in various industries such as food, paper, electronic and biomedical. However, the current productivity is still very low. Therefore, it is imperative to seek for BC production by utilizing other sources. Therefore, the main objectives of this work were (i) to quanti...

Full description

Saved in:

Bibliographic Details
Main Author:	Lotfiman, Samaneh
Format:	Thesis
Language:	English
Published:	2010
Subjects:	Bacteria Date sugar Cellulose
Online Access:	http://psasir.upm.edu.my/id/eprint/41170/1/FK%202010%2080R.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-upm-ir.41170
record_format	uketd_dc
spelling	my-upm-ir.411702015-10-28T08:49:00Z Bacterial cellulose production from extracted date syrup 2010-11 Lotfiman, Samaneh Bacterial cellulose (BC) is used in various industries such as food, paper, electronic and biomedical. However, the current productivity is still very low. Therefore, it is imperative to seek for BC production by utilizing other sources. Therefore, the main objectives of this work were (i) to quantify and characterized sugars from date using various extraction methods, (ii) to optimize bacterial cellulose production using the extracted sugars as substrate, (iii) to characterize and quantify bacterial cellulose production from the various extraction methods used. It is predicted that replacing glucose in Hestrin and Schramm medium with extracted date syrup will alter the specific properties of the BC produced. To achieve these objectives, different sugar extraction methods were compared, namely stirred and ultrasonic-assisted water extraction, and solvent extraction using 50% ethanol and 80% methanol as solvents. The results of the work showed that sugar extraction from date by water with ultrasonic assisted, extracted the highest amount of total sugar (79%). The amount of extracted total sugar via water (stirring), ethanol and methanol were 71%, 67% and 57% respectively. Furthermore, the results revealed that BC was formed in the medium where date syrup was used as a carbon source. The enriched medium utilizing extracted syrup produced from 30 g dry date/l via ultrasonic assisted water extraction method was able to produce 186% BC more than that produced in Hestrin and Schramm medium. SEM analysis of the BCs showed fine nanofibers (60-90 nm) and smooth surface. The presence of C=O amid bond and one of collagen features, in the BC exhibited by FTIR demonstrated that the BC produced in the enriched medium has the potential to be used in medical application, i.e. wound dressing. The BC mechanical tests results revealed considerable improvement in the Young Modulus, tensile stress and elongation at break values over the BC formed in the Hestrin and Schramm medium, namely; 29-80%, 50-140% and 33-119% respectively. To conclude, this work has successfully achieved the outlined objectives. The properties of the BC characterized illustrated that BC can be re-engineered by using date fruit as a carbon source; this has created a more promising future for BC especially in medical applications. Bacteria Date sugar Cellulose 2010-11 Thesis http://psasir.upm.edu.my/id/eprint/41170/ http://psasir.upm.edu.my/id/eprint/41170/1/FK%202010%2080R.pdf application/pdf en public masters Universiti Putra Malaysia Bacteria Date sugar Cellulose
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Bacteria Date sugar Cellulose
spellingShingle	Bacteria Date sugar Cellulose Lotfiman, Samaneh Bacterial cellulose production from extracted date syrup
description	Bacterial cellulose (BC) is used in various industries such as food, paper, electronic and biomedical. However, the current productivity is still very low. Therefore, it is imperative to seek for BC production by utilizing other sources. Therefore, the main objectives of this work were (i) to quantify and characterized sugars from date using various extraction methods, (ii) to optimize bacterial cellulose production using the extracted sugars as substrate, (iii) to characterize and quantify bacterial cellulose production from the various extraction methods used. It is predicted that replacing glucose in Hestrin and Schramm medium with extracted date syrup will alter the specific properties of the BC produced. To achieve these objectives, different sugar extraction methods were compared, namely stirred and ultrasonic-assisted water extraction, and solvent extraction using 50% ethanol and 80% methanol as solvents. The results of the work showed that sugar extraction from date by water with ultrasonic assisted, extracted the highest amount of total sugar (79%). The amount of extracted total sugar via water (stirring), ethanol and methanol were 71%, 67% and 57% respectively. Furthermore, the results revealed that BC was formed in the medium where date syrup was used as a carbon source. The enriched medium utilizing extracted syrup produced from 30 g dry date/l via ultrasonic assisted water extraction method was able to produce 186% BC more than that produced in Hestrin and Schramm medium. SEM analysis of the BCs showed fine nanofibers (60-90 nm) and smooth surface. The presence of C=O amid bond and one of collagen features, in the BC exhibited by FTIR demonstrated that the BC produced in the enriched medium has the potential to be used in medical application, i.e. wound dressing. The BC mechanical tests results revealed considerable improvement in the Young Modulus, tensile stress and elongation at break values over the BC formed in the Hestrin and Schramm medium, namely; 29-80%, 50-140% and 33-119% respectively. To conclude, this work has successfully achieved the outlined objectives. The properties of the BC characterized illustrated that BC can be re-engineered by using date fruit as a carbon source; this has created a more promising future for BC especially in medical applications.
format	Thesis
qualification_level	Master's degree
author	Lotfiman, Samaneh
author_facet	Lotfiman, Samaneh
author_sort	Lotfiman, Samaneh
title	Bacterial cellulose production from extracted date syrup
title_short	Bacterial cellulose production from extracted date syrup
title_full	Bacterial cellulose production from extracted date syrup
title_fullStr	Bacterial cellulose production from extracted date syrup
title_full_unstemmed	Bacterial cellulose production from extracted date syrup
title_sort	bacterial cellulose production from extracted date syrup
granting_institution	Universiti Putra Malaysia
publishDate	2010
url	http://psasir.upm.edu.my/id/eprint/41170/1/FK%202010%2080R.pdf
_version_	1747811858210881536

Bacterial cellulose production from extracted date syrup

Similar Items