Incidence and differentiation of Cucumber mosaic virus (CMV) isolates in Peninsular Malaysia

A survey on the occurrence and incidence of Cucumber mosaic virus (CMV) was carried out on selected horticultural crops in Peninsular Malaysia. A total of 1293 leaf samples showing virus-like symptoms comprising of chilli (527 samples), tomato (63 samples), eggplant (183 samples), cucumber (209 sam...

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Main Author:	Saad, Norsazilawati
Format:	Thesis
Language:	English
Published:	2012
Subjects:	Cucumber mosaic virus - Malaysia Horticultural crops - Malaysia Nucleotide sequence
Online Access:	http://psasir.upm.edu.my/id/eprint/49344/7/FP%202012%2080RR.pdf
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spelling	my-upm-ir.493442017-01-25T02:49:18Z Incidence and differentiation of Cucumber mosaic virus (CMV) isolates in Peninsular Malaysia 2012-04 Saad, Norsazilawati A survey on the occurrence and incidence of Cucumber mosaic virus (CMV) was carried out on selected horticultural crops in Peninsular Malaysia. A total of 1293 leaf samples showing virus-like symptoms comprising of chilli (527 samples), tomato (63 samples), eggplant (183 samples), cucumber (209 samples), pineapple (161 samples), long bean (49 samples), and loofah (101 samples) were collected from the field at 35 different locations in 7 states (Selangor, Pahang, Melaka, Johor, Kelantan, Perak and Pulau Pinang) to detect the presence of CMV. The detection of CMV was done using double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). Based on the DAS-ELISA results, CMV was detected in 9% of the entire surveyed crops. The highest percentage of CMV incidences was found in Selangor with 48% infection, followed by Kelantan and Melaka (15%),Pahang (8%), Johor (7%), Pulau Pinang (4%), and Perak (1%). Amongst the samples collected, CMV was discovered to be the most prevalent on eggplant and chilli with 12% disease incidence each, followed by tomato (10%), cucumber (5%), long beans (4%) and pineapple and loofah (3% each). CMV-infected samples detected by DAS-ELISA were used in reversetranscription polymerase chain reaction (RT-PCR) analysis. Gene-specific primers were designed and a primer was then selected to amplify the region of coat protein (CP) gene, producing a specific band sized of approximately 600 base pair (bp). Samples used for RT-PCR included crops collected from Selangor, Pahang, Melaka, Johor, Kelantan, Perak, Pulau Pinang and with an addition of samples from Terengganu. Only crops collected from Johor (long beans, eggplant, cucumber, chilli and pineapple), Pahang (chilli, tomato, cucumber and eggplant), Terengganu (chilli, loofah and eggplant),Perak (cucumber), Pulau Pinang (chilli) and Selangor (chilli, tomato and eggplant) produced DNA bands of approximately 600 bp in size. Nucleotide sequences of the CP gene of 20 CMV isolates from tomato,eggplant, loofah, chilli and tobacco collected in Terengganu, Pahang,Penang, Perak and Selangor were confirmed as CMV. Phylogenetic analysis of the partial CP genes suggest that the local CMV isolates belong to subgroup I (S-I) and are grouped together with the subgroup IB (S-IB) strains. The results further proposed that the isolates can be arranged according to the type of crops and geographical location. Cucumber mosaic virus - Malaysia Horticultural crops - Malaysia Nucleotide sequence 2012-04 Thesis http://psasir.upm.edu.my/id/eprint/49344/ http://psasir.upm.edu.my/id/eprint/49344/7/FP%202012%2080RR.pdf application/pdf en public masters Universiti Putra Malaysia Cucumber mosaic virus - Malaysia Horticultural crops - Malaysia Nucleotide sequence
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Cucumber mosaic virus - Malaysia Horticultural crops - Malaysia Nucleotide sequence
spellingShingle	Cucumber mosaic virus - Malaysia Horticultural crops - Malaysia Nucleotide sequence Saad, Norsazilawati Incidence and differentiation of Cucumber mosaic virus (CMV) isolates in Peninsular Malaysia
description	A survey on the occurrence and incidence of Cucumber mosaic virus (CMV) was carried out on selected horticultural crops in Peninsular Malaysia. A total of 1293 leaf samples showing virus-like symptoms comprising of chilli (527 samples), tomato (63 samples), eggplant (183 samples), cucumber (209 samples), pineapple (161 samples), long bean (49 samples), and loofah (101 samples) were collected from the field at 35 different locations in 7 states (Selangor, Pahang, Melaka, Johor, Kelantan, Perak and Pulau Pinang) to detect the presence of CMV. The detection of CMV was done using double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). Based on the DAS-ELISA results, CMV was detected in 9% of the entire surveyed crops. The highest percentage of CMV incidences was found in Selangor with 48% infection, followed by Kelantan and Melaka (15%),Pahang (8%), Johor (7%), Pulau Pinang (4%), and Perak (1%). Amongst the samples collected, CMV was discovered to be the most prevalent on eggplant and chilli with 12% disease incidence each, followed by tomato (10%), cucumber (5%), long beans (4%) and pineapple and loofah (3% each). CMV-infected samples detected by DAS-ELISA were used in reversetranscription polymerase chain reaction (RT-PCR) analysis. Gene-specific primers were designed and a primer was then selected to amplify the region of coat protein (CP) gene, producing a specific band sized of approximately 600 base pair (bp). Samples used for RT-PCR included crops collected from Selangor, Pahang, Melaka, Johor, Kelantan, Perak, Pulau Pinang and with an addition of samples from Terengganu. Only crops collected from Johor (long beans, eggplant, cucumber, chilli and pineapple), Pahang (chilli, tomato, cucumber and eggplant), Terengganu (chilli, loofah and eggplant),Perak (cucumber), Pulau Pinang (chilli) and Selangor (chilli, tomato and eggplant) produced DNA bands of approximately 600 bp in size. Nucleotide sequences of the CP gene of 20 CMV isolates from tomato,eggplant, loofah, chilli and tobacco collected in Terengganu, Pahang,Penang, Perak and Selangor were confirmed as CMV. Phylogenetic analysis of the partial CP genes suggest that the local CMV isolates belong to subgroup I (S-I) and are grouped together with the subgroup IB (S-IB) strains. The results further proposed that the isolates can be arranged according to the type of crops and geographical location.
format	Thesis
qualification_level	Master's degree
author	Saad, Norsazilawati
author_facet	Saad, Norsazilawati
author_sort	Saad, Norsazilawati
title	Incidence and differentiation of Cucumber mosaic virus (CMV) isolates in Peninsular Malaysia
title_short	Incidence and differentiation of Cucumber mosaic virus (CMV) isolates in Peninsular Malaysia
title_full	Incidence and differentiation of Cucumber mosaic virus (CMV) isolates in Peninsular Malaysia
title_fullStr	Incidence and differentiation of Cucumber mosaic virus (CMV) isolates in Peninsular Malaysia
title_full_unstemmed	Incidence and differentiation of Cucumber mosaic virus (CMV) isolates in Peninsular Malaysia
title_sort	incidence and differentiation of cucumber mosaic virus (cmv) isolates in peninsular malaysia
granting_institution	Universiti Putra Malaysia
publishDate	2012
url	http://psasir.upm.edu.my/id/eprint/49344/7/FP%202012%2080RR.pdf
_version_	1747812011401543680

Incidence and differentiation of Cucumber mosaic virus (CMV) isolates in Peninsular Malaysia

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