Physiological Responses of Misai Kucing (Orthosiphon Stamineus Benth) Under Different Light Intensities

The effect of shading on the growth, physiological parameters, biomass content and antioxidant activities of local Misai kucing, Orthosiphon stamineus was investigated. The growth of physiological parameters of O. stamineus was measured according to the position of the leaves on different node. The...

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Main Author:	Abd Aziz, Fatma Azwani
Format:	Thesis
Language:	English English
Published:	2007
Subjects:	Plants - Effect of light on. Light - Physiological effect.
Online Access:	http://psasir.upm.edu.my/id/eprint/5082/1/FS_2007_56.pdf
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spelling	my-upm-ir.50822013-05-27T07:20:16Z Physiological Responses of Misai Kucing (Orthosiphon Stamineus Benth) Under Different Light Intensities 2007 Abd Aziz, Fatma Azwani The effect of shading on the growth, physiological parameters, biomass content and antioxidant activities of local Misai kucing, Orthosiphon stamineus was investigated. The growth of physiological parameters of O. stamineus was measured according to the position of the leaves on different node. The O. stamineus was grown in three different shading conditions that were 0% (receiving direct sunlight), 50% and 70% of direct sunlight by mean of shading. Black nylon netting commonly used in plant nursery that reduced 50% and 70% of the incident light cover the plants. Approximately 10 cm in length of O. stamineus cuttings were immersed in a jar filled with tap water to induce root formation before transferring to 10 x 12 cm polybag containing mixture of loam (Kosas-Peat) with organic compost (Amino-Q) in the ratio of 2:1. Common agronomic parameters and physiological parameters were recorded for every fortnight. Method used for determining the antioxidant activity was β-carotene bleaching method, whilst samples were extracted using an aqueous (water) and 80% methanol. Results showed that the exposure to the direct sunlight (0% shading) and 70% light reduction stunted the growth of O. stamineus. The biomass content was the highest under 50% shading treatment. Based on the observation, 50% shading was recommended for optimum growing condition for O. stamineus. It shows that 0% shading gives the highest leaf’s transpiration rate and stomatal conductance whilst chlorophyll concentration on leaf of O. stamineus was found at the highest-level under 70% shading. Comparison between leaves on node 3, 4, 5 and 6 respectively showed that the transpiration rate decreased towards the lower position. From the three shading treatments, it was found that the transpiration rate and stomatal conductance of the leaf was highest on leaf 3 whilst leaf 6 contained the highest chlorophyll concentration. The exposure to the high light intensity increased the transpiration rate and stomatal conductance and reduced the total chlorophyll of O. stamineus leaf. The result showed that the highest activities of antioxidant are on sixtieth minutes which was chosen to determine the comparison on activities of antioxidant between three different light exposure. The exposure under the 70% shading showed the highest percentage of antioxidant activities on leaf 6 (79.89%) for aqueous extract and leaf 4 (83.81%) for the organic extract. Plants - Effect of light on. Light - Physiological effect. 2007 Thesis http://psasir.upm.edu.my/id/eprint/5082/ http://psasir.upm.edu.my/id/eprint/5082/1/FS_2007_56.pdf application/pdf en public masters Universiti Putra Malaysia Plants - Effect of light on. Light - Physiological effect. Science English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Plants - Effect of light on. Light - Physiological effect.
spellingShingle	Plants - Effect of light on. Light - Physiological effect. Abd Aziz, Fatma Azwani Physiological Responses of Misai Kucing (Orthosiphon Stamineus Benth) Under Different Light Intensities
description	The effect of shading on the growth, physiological parameters, biomass content and antioxidant activities of local Misai kucing, Orthosiphon stamineus was investigated. The growth of physiological parameters of O. stamineus was measured according to the position of the leaves on different node. The O. stamineus was grown in three different shading conditions that were 0% (receiving direct sunlight), 50% and 70% of direct sunlight by mean of shading. Black nylon netting commonly used in plant nursery that reduced 50% and 70% of the incident light cover the plants. Approximately 10 cm in length of O. stamineus cuttings were immersed in a jar filled with tap water to induce root formation before transferring to 10 x 12 cm polybag containing mixture of loam (Kosas-Peat) with organic compost (Amino-Q) in the ratio of 2:1. Common agronomic parameters and physiological parameters were recorded for every fortnight. Method used for determining the antioxidant activity was β-carotene bleaching method, whilst samples were extracted using an aqueous (water) and 80% methanol. Results showed that the exposure to the direct sunlight (0% shading) and 70% light reduction stunted the growth of O. stamineus. The biomass content was the highest under 50% shading treatment. Based on the observation, 50% shading was recommended for optimum growing condition for O. stamineus. It shows that 0% shading gives the highest leaf’s transpiration rate and stomatal conductance whilst chlorophyll concentration on leaf of O. stamineus was found at the highest-level under 70% shading. Comparison between leaves on node 3, 4, 5 and 6 respectively showed that the transpiration rate decreased towards the lower position. From the three shading treatments, it was found that the transpiration rate and stomatal conductance of the leaf was highest on leaf 3 whilst leaf 6 contained the highest chlorophyll concentration. The exposure to the high light intensity increased the transpiration rate and stomatal conductance and reduced the total chlorophyll of O. stamineus leaf. The result showed that the highest activities of antioxidant are on sixtieth minutes which was chosen to determine the comparison on activities of antioxidant between three different light exposure. The exposure under the 70% shading showed the highest percentage of antioxidant activities on leaf 6 (79.89%) for aqueous extract and leaf 4 (83.81%) for the organic extract.
format	Thesis
qualification_level	Master's degree
author	Abd Aziz, Fatma Azwani
author_facet	Abd Aziz, Fatma Azwani
author_sort	Abd Aziz, Fatma Azwani
title	Physiological Responses of Misai Kucing (Orthosiphon Stamineus Benth) Under Different Light Intensities
title_short	Physiological Responses of Misai Kucing (Orthosiphon Stamineus Benth) Under Different Light Intensities
title_full	Physiological Responses of Misai Kucing (Orthosiphon Stamineus Benth) Under Different Light Intensities
title_fullStr	Physiological Responses of Misai Kucing (Orthosiphon Stamineus Benth) Under Different Light Intensities
title_full_unstemmed	Physiological Responses of Misai Kucing (Orthosiphon Stamineus Benth) Under Different Light Intensities
title_sort	physiological responses of misai kucing (orthosiphon stamineus benth) under different light intensities
granting_institution	Universiti Putra Malaysia
granting_department	Science
publishDate	2007
url	http://psasir.upm.edu.my/id/eprint/5082/1/FS_2007_56.pdf
_version_	1747810347130028032

Physiological Responses of Misai Kucing (Orthosiphon Stamineus Benth) Under Different Light Intensities

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