Development of bioorganic fertilizer containing Pseudomonas GanoEB3 in promoting oil palm growth and inhibiting Ganoderma boninense

Oil palm is one of the important crops in Malaysia. Basal Stem Rot (BSR) disease is the biggest threat for oil palm production in Malaysia caused by Ganoderma boninense has been causing a huge damage for the oil palm industry in Malaysia. The objective of this study was to develop the bioorganic fer...

Full description

Saved in:

Bibliographic Details
Main Author:	Abdul Wahab, Nurul Isma
Format:	Thesis
Language:	English
Published:	2014
Subjects:	Organic fertilizers Pseudomonas Ganoderma
Online Access:	http://psasir.upm.edu.my/id/eprint/52097/1/FS%202014%2011RR.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-upm-ir.52097
record_format	uketd_dc
spelling	my-upm-ir.520972017-05-03T03:31:09Z Development of bioorganic fertilizer containing Pseudomonas GanoEB3 in promoting oil palm growth and inhibiting Ganoderma boninense 2014-06 Abdul Wahab, Nurul Isma Oil palm is one of the important crops in Malaysia. Basal Stem Rot (BSR) disease is the biggest threat for oil palm production in Malaysia caused by Ganoderma boninense has been causing a huge damage for the oil palm industry in Malaysia. The objective of this study was to develop the bioorganic fertilizer containing Pseudomonas GanoEB3 as oil palm growth enhancer and for inhibiting Ganoderma boninense. Endophytic bacteria Pseudomonas GanoEB3 was isolated from healthy oil palm roots,and cultured on nutrient agar media. A suspension containing 108 CFU/mL of the bacteria cells being mixed with vermiculite powder and stored at room temperature. The vermiculite powder (V) containing Pseudomonas GanoEB3 has been formulated with Bioorganic EFB and Bioorganic BF with following ratios; 0 g (EFB/BF): 60 g (V), 10 g (EFB/BF): 50 g (V), 20 g (EFB/BF): 40 g (V), 30 g (EFB/BF): 30 g (V), 40 g (EFB/BF): 20 g (V), 50 g (EFB/BF): 10 g (V) and 60 g (EFB/BF): 0 g (V). This study found that 30 g Bioorganic EFB mixed with 30 g vermiculite containing Pseudomonas GanoEB3 showed the best viability test with the percentage inhibition of radial growth (PIRG) value of 57.75% against G. boninense after one day product storage. Whereas, the PIRG value was 14.38% after twelve months product storage. Meanwhile, 10 g Bioorganic BF mixed with 50 g vermiculite containing Pseudomonas GanoEB3 showed 53.75% and 18% PIRG value after one day and twelve months product storage, respectively. Plant growth result showed that seedlings treated with Bioorganic BF gave the highest result in plant height (91.61 ± 0.80 cm), girth (46.86 ± 0.49 mm), frond length (64.61 ± 0.58 cm),number of leaflets (17.67 ± 0.30), number of frond (14.33 ± 0.18), chlorophyll content (54.95 ± 0.47 μg/L), leaf area index (2175.30 ± 88.08 cm2) and biomass (53.97 ± 0.76 g) respectively, followed by seedlings treated with Bioorganic EFB and control treatment. Pathological analysis showed that Bioorganic EFB containing Pseudomonas GanoEB3 (T3) and Bioorganic BF containing Pseudomonas GanoEB3 (T4) also have a good potential in inhibiting the growth of G. boninense. The reduced percentage of disease incidence (DI) for T3 (40%) and T4 (50%), area under the disease progress curve (AUDPC) for T3 (42 units2) and T4 (76 units2), disease severity of foliar index (DSFI) for T3 (37.5%) and T4 (45%), disease severity of bole index (DSBI) for T3 (40%) and T4 (47.5%), disease severity of root index (DSRI) for T3 (55%) and T4 (52.5%) and dead seedlings for T3 (30%) and T4 (40%) compared with control treatment (T2). It shows that both treatments have a good potential in inhibiting BSR disease in oil palm seedlings. This study revealed that Bioorganic EFB and Bioorganic BF containing Pseudomonas GanoEB3 are suitable as an effective biological control agent for promoting the growth of oil palm seedlings and suppressing G. boninense. Organic fertilizers Pseudomonas Ganoderma 2014-06 Thesis http://psasir.upm.edu.my/id/eprint/52097/ http://psasir.upm.edu.my/id/eprint/52097/1/FS%202014%2011RR.pdf application/pdf en public masters Universiti Putra Malaysia Organic fertilizers Pseudomonas Ganoderma
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Organic fertilizers Pseudomonas Ganoderma
spellingShingle	Organic fertilizers Pseudomonas Ganoderma Abdul Wahab, Nurul Isma Development of bioorganic fertilizer containing Pseudomonas GanoEB3 in promoting oil palm growth and inhibiting Ganoderma boninense
description	Oil palm is one of the important crops in Malaysia. Basal Stem Rot (BSR) disease is the biggest threat for oil palm production in Malaysia caused by Ganoderma boninense has been causing a huge damage for the oil palm industry in Malaysia. The objective of this study was to develop the bioorganic fertilizer containing Pseudomonas GanoEB3 as oil palm growth enhancer and for inhibiting Ganoderma boninense. Endophytic bacteria Pseudomonas GanoEB3 was isolated from healthy oil palm roots,and cultured on nutrient agar media. A suspension containing 108 CFU/mL of the bacteria cells being mixed with vermiculite powder and stored at room temperature. The vermiculite powder (V) containing Pseudomonas GanoEB3 has been formulated with Bioorganic EFB and Bioorganic BF with following ratios; 0 g (EFB/BF): 60 g (V), 10 g (EFB/BF): 50 g (V), 20 g (EFB/BF): 40 g (V), 30 g (EFB/BF): 30 g (V), 40 g (EFB/BF): 20 g (V), 50 g (EFB/BF): 10 g (V) and 60 g (EFB/BF): 0 g (V). This study found that 30 g Bioorganic EFB mixed with 30 g vermiculite containing Pseudomonas GanoEB3 showed the best viability test with the percentage inhibition of radial growth (PIRG) value of 57.75% against G. boninense after one day product storage. Whereas, the PIRG value was 14.38% after twelve months product storage. Meanwhile, 10 g Bioorganic BF mixed with 50 g vermiculite containing Pseudomonas GanoEB3 showed 53.75% and 18% PIRG value after one day and twelve months product storage, respectively. Plant growth result showed that seedlings treated with Bioorganic BF gave the highest result in plant height (91.61 ± 0.80 cm), girth (46.86 ± 0.49 mm), frond length (64.61 ± 0.58 cm),number of leaflets (17.67 ± 0.30), number of frond (14.33 ± 0.18), chlorophyll content (54.95 ± 0.47 μg/L), leaf area index (2175.30 ± 88.08 cm2) and biomass (53.97 ± 0.76 g) respectively, followed by seedlings treated with Bioorganic EFB and control treatment. Pathological analysis showed that Bioorganic EFB containing Pseudomonas GanoEB3 (T3) and Bioorganic BF containing Pseudomonas GanoEB3 (T4) also have a good potential in inhibiting the growth of G. boninense. The reduced percentage of disease incidence (DI) for T3 (40%) and T4 (50%), area under the disease progress curve (AUDPC) for T3 (42 units2) and T4 (76 units2), disease severity of foliar index (DSFI) for T3 (37.5%) and T4 (45%), disease severity of bole index (DSBI) for T3 (40%) and T4 (47.5%), disease severity of root index (DSRI) for T3 (55%) and T4 (52.5%) and dead seedlings for T3 (30%) and T4 (40%) compared with control treatment (T2). It shows that both treatments have a good potential in inhibiting BSR disease in oil palm seedlings. This study revealed that Bioorganic EFB and Bioorganic BF containing Pseudomonas GanoEB3 are suitable as an effective biological control agent for promoting the growth of oil palm seedlings and suppressing G. boninense.
format	Thesis
qualification_level	Master's degree
author	Abdul Wahab, Nurul Isma
author_facet	Abdul Wahab, Nurul Isma
author_sort	Abdul Wahab, Nurul Isma
title	Development of bioorganic fertilizer containing Pseudomonas GanoEB3 in promoting oil palm growth and inhibiting Ganoderma boninense
title_short	Development of bioorganic fertilizer containing Pseudomonas GanoEB3 in promoting oil palm growth and inhibiting Ganoderma boninense
title_full	Development of bioorganic fertilizer containing Pseudomonas GanoEB3 in promoting oil palm growth and inhibiting Ganoderma boninense
title_fullStr	Development of bioorganic fertilizer containing Pseudomonas GanoEB3 in promoting oil palm growth and inhibiting Ganoderma boninense
title_full_unstemmed	Development of bioorganic fertilizer containing Pseudomonas GanoEB3 in promoting oil palm growth and inhibiting Ganoderma boninense
title_sort	development of bioorganic fertilizer containing pseudomonas ganoeb3 in promoting oil palm growth and inhibiting ganoderma boninense
granting_institution	Universiti Putra Malaysia
publishDate	2014
url	http://psasir.upm.edu.my/id/eprint/52097/1/FS%202014%2011RR.pdf
_version_	1747812091454029824

Development of bioorganic fertilizer containing Pseudomonas GanoEB3 in promoting oil palm growth and inhibiting Ganoderma boninense

Similar Items