Potential Of Selected Antagonistic Bacteria In Controlling Damping-Off Disease Of Brassica Chinensis L. Caused By Pythium Myriotylum Drech

The causal agent of damping-off of Brassica chinensis was isolated and identified as Pythium myriotylum Drech. based on its morphological characteristics. Isolation of bacteria from the rhizosphere region of B. chinensis seedlings resulted in the isolation of 118 bacterial isolates where 6 isolates...

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Main Author:	Zahara, Hafni
Format:	Thesis
Language:	English English
Published:	2004
Subjects:	Bok choy Antagonistic fungi
Online Access:	http://psasir.upm.edu.my/id/eprint/5472/1/FP_2004_32.pdf
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id	my-upm-ir.5472
record_format	uketd_dc
spelling	my-upm-ir.54722013-05-27T07:23:05Z Potential Of Selected Antagonistic Bacteria In Controlling Damping-Off Disease Of Brassica Chinensis L. Caused By Pythium Myriotylum Drech 2004 Zahara, Hafni The causal agent of damping-off of Brassica chinensis was isolated and identified as Pythium myriotylum Drech. based on its morphological characteristics. Isolation of bacteria from the rhizosphere region of B. chinensis seedlings resulted in the isolation of 118 bacterial isolates where 6 isolates were found to have antagonistic activities against P. myriotylum.Using the Biolog® Identification System, these antagonistic bacteria were identified as Serratia marcescens, Rhodococcus erythropolis, Tsukamurella inchonensis, Burkholderia cepacia (Syn. Pseudomonas cepacia), Pseudomonas aeruginosa 1 and Pseudomonas aeruginosa 2. Based on dual culture test, B. cepacia was the best antagonist in inhibiting the mycelial growth of P. myriotylum.. B. cepacia inhibited the mycelial growth of P.myriotylum (24.50 mm) based on dual culture and clearing zone inhibition of 80.00 mm, based on colony degradation tests. In double media overlay tests, B. cepacia, P. aeruginosa 1 and P. aeruginosa 2 were antagonistic with no radial mycelial growth of P. myriotylum. Experiments in the screenhouse indicated that B. cepacia was more effective in protecting the B. chinensis seeds against P. myriotylum than P. aeruginosa 1 and P. aeruginosa 2, and was equally effective as fungicide Captan®. Soil treatment was more effective than seed treatment in increasing the percentage seedling emergence, percentage survival plants and dry weight (g) of B. chinensis. Bok choy Antagonistic fungi 2004 Thesis http://psasir.upm.edu.my/id/eprint/5472/ http://psasir.upm.edu.my/id/eprint/5472/1/FP_2004_32.pdf application/pdf en public masters Universiti Putra Malaysia Bok choy Antagonistic fungi Faculty Agriculture English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Bok choy Antagonistic fungi
spellingShingle	Bok choy Antagonistic fungi Zahara, Hafni Potential Of Selected Antagonistic Bacteria In Controlling Damping-Off Disease Of Brassica Chinensis L. Caused By Pythium Myriotylum Drech
description	The causal agent of damping-off of Brassica chinensis was isolated and identified as Pythium myriotylum Drech. based on its morphological characteristics. Isolation of bacteria from the rhizosphere region of B. chinensis seedlings resulted in the isolation of 118 bacterial isolates where 6 isolates were found to have antagonistic activities against P. myriotylum.Using the Biolog® Identification System, these antagonistic bacteria were identified as Serratia marcescens, Rhodococcus erythropolis, Tsukamurella inchonensis, Burkholderia cepacia (Syn. Pseudomonas cepacia), Pseudomonas aeruginosa 1 and Pseudomonas aeruginosa 2. Based on dual culture test, B. cepacia was the best antagonist in inhibiting the mycelial growth of P. myriotylum.. B. cepacia inhibited the mycelial growth of P.myriotylum (24.50 mm) based on dual culture and clearing zone inhibition of 80.00 mm, based on colony degradation tests. In double media overlay tests, B. cepacia, P. aeruginosa 1 and P. aeruginosa 2 were antagonistic with no radial mycelial growth of P. myriotylum. Experiments in the screenhouse indicated that B. cepacia was more effective in protecting the B. chinensis seeds against P. myriotylum than P. aeruginosa 1 and P. aeruginosa 2, and was equally effective as fungicide Captan®. Soil treatment was more effective than seed treatment in increasing the percentage seedling emergence, percentage survival plants and dry weight (g) of B. chinensis.
format	Thesis
qualification_level	Master's degree
author	Zahara, Hafni
author_facet	Zahara, Hafni
author_sort	Zahara, Hafni
title	Potential Of Selected Antagonistic Bacteria In Controlling Damping-Off Disease Of Brassica Chinensis L. Caused By Pythium Myriotylum Drech
title_short	Potential Of Selected Antagonistic Bacteria In Controlling Damping-Off Disease Of Brassica Chinensis L. Caused By Pythium Myriotylum Drech
title_full	Potential Of Selected Antagonistic Bacteria In Controlling Damping-Off Disease Of Brassica Chinensis L. Caused By Pythium Myriotylum Drech
title_fullStr	Potential Of Selected Antagonistic Bacteria In Controlling Damping-Off Disease Of Brassica Chinensis L. Caused By Pythium Myriotylum Drech
title_full_unstemmed	Potential Of Selected Antagonistic Bacteria In Controlling Damping-Off Disease Of Brassica Chinensis L. Caused By Pythium Myriotylum Drech
title_sort	potential of selected antagonistic bacteria in controlling damping-off disease of brassica chinensis l. caused by pythium myriotylum drech
granting_institution	Universiti Putra Malaysia
granting_department	Faculty Agriculture
publishDate	2004
url	http://psasir.upm.edu.my/id/eprint/5472/1/FP_2004_32.pdf
_version_	1747810432995819520

Potential Of Selected Antagonistic Bacteria In Controlling Damping-Off Disease Of Brassica Chinensis L. Caused By Pythium Myriotylum Drech

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