Genotypic Characterization of Vibrio Sp. Isolated from Cockles Obtained in Padang, Indonesia

Vibrio parahaemoi'yticus is a gram negative bacterium that naturally found in warm marine environments. They commonly live in shellfish, oysters and cockles and cause gastrointestinal illness in humans. Most people become infected by eating raw or undercooked shellfish. In this study, 32 iso...

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Main Author:	Yaakub, Zulkifli
Format:	Thesis
Language:	English English
Published:	2006
Subjects:	Nuttall cockle - Indonesia
Online Access:	http://psasir.upm.edu.my/id/eprint/5992/1/FBSB_2006_19.pdf
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spelling	my-upm-ir.59922023-01-03T07:25:37Z Genotypic Characterization of Vibrio Sp. Isolated from Cockles Obtained in Padang, Indonesia 2006-03 Yaakub, Zulkifli Vibrio parahaemoi'yticus is a gram negative bacterium that naturally found in warm marine environments. They commonly live in shellfish, oysters and cockles and cause gastrointestinal illness in humans. Most people become infected by eating raw or undercooked shellfish. In this study, 32 isolates of V: parahaemolyticus were isolated Erom cockles obtained from Padang, Indonesia. Presumptive identification of the isolates was performed by the following analysis: antibiotic resistance, plasmid profiling, specific PCR, random amplified polymorphic DNA (RAPD)-PCR and enternbacterial repetitive intergenic consensus (ERIC)-PCR. In the antibiotic resistance test, 16 types of antibiotics were tested against this bacterium. The isolates of V: parahemolyticus showed variable antibiotic resistance pattern. They are most commonly resistant to amoxycillin, bacitracin, penicillin, teicoplanin, ampicillin and carbenicillin. In the plasmid profiling analysis, 14 different profiles were determined. The most common profiles for the isolates is profile 110.2 which contains 4 plasmids with 5.6,7.2,42.4 and 54.0 kilobase pairs respectively. For toxR detection, all of the isolates were positive but they gave negative results in both tdh and trh detection. This indicated that they have less potential to cause diseases. They also showed high diversity in RAPD-PCR and also ERIC-PCR analysis. They showed many differences in the polymorphism pattern and can be divided into 5 major clusters in RAPD-PCR and 4 major clusters in ERIC-PCR. Application of phenotype and genotype based methods provides information about distribution, epidemiology and pathogenesis of V. parasympathetics. The data is important to identify sources of infection of this bacterium in the case of outbreaks occurrence in humans. This study also gives us preliminary information in monitoring the potential infection risk. It also can increase our awareness about the infection potential of these bacteria. Nuttall cockle - Indonesia 2006-03 Thesis http://psasir.upm.edu.my/id/eprint/5992/ http://psasir.upm.edu.my/id/eprint/5992/1/FBSB_2006_19.pdf text en public masters Universiti Putra Malaysia Nuttall cockle - Indonesia Faculty of Biotechnology and Biomokular Sciences English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Nuttall cockle - Indonesia
spellingShingle	Nuttall cockle - Indonesia Yaakub, Zulkifli Genotypic Characterization of Vibrio Sp. Isolated from Cockles Obtained in Padang, Indonesia
description	Vibrio parahaemoi'yticus is a gram negative bacterium that naturally found in warm marine environments. They commonly live in shellfish, oysters and cockles and cause gastrointestinal illness in humans. Most people become infected by eating raw or undercooked shellfish. In this study, 32 isolates of V: parahaemolyticus were isolated Erom cockles obtained from Padang, Indonesia. Presumptive identification of the isolates was performed by the following analysis: antibiotic resistance, plasmid profiling, specific PCR, random amplified polymorphic DNA (RAPD)-PCR and enternbacterial repetitive intergenic consensus (ERIC)-PCR. In the antibiotic resistance test, 16 types of antibiotics were tested against this bacterium. The isolates of V: parahemolyticus showed variable antibiotic resistance pattern. They are most commonly resistant to amoxycillin, bacitracin, penicillin, teicoplanin, ampicillin and carbenicillin. In the plasmid profiling analysis, 14 different profiles were determined. The most common profiles for the isolates is profile 110.2 which contains 4 plasmids with 5.6,7.2,42.4 and 54.0 kilobase pairs respectively. For toxR detection, all of the isolates were positive but they gave negative results in both tdh and trh detection. This indicated that they have less potential to cause diseases. They also showed high diversity in RAPD-PCR and also ERIC-PCR analysis. They showed many differences in the polymorphism pattern and can be divided into 5 major clusters in RAPD-PCR and 4 major clusters in ERIC-PCR. Application of phenotype and genotype based methods provides information about distribution, epidemiology and pathogenesis of V. parasympathetics. The data is important to identify sources of infection of this bacterium in the case of outbreaks occurrence in humans. This study also gives us preliminary information in monitoring the potential infection risk. It also can increase our awareness about the infection potential of these bacteria.
format	Thesis
qualification_level	Master's degree
author	Yaakub, Zulkifli
author_facet	Yaakub, Zulkifli
author_sort	Yaakub, Zulkifli
title	Genotypic Characterization of Vibrio Sp. Isolated from Cockles Obtained in Padang, Indonesia
title_short	Genotypic Characterization of Vibrio Sp. Isolated from Cockles Obtained in Padang, Indonesia
title_full	Genotypic Characterization of Vibrio Sp. Isolated from Cockles Obtained in Padang, Indonesia
title_fullStr	Genotypic Characterization of Vibrio Sp. Isolated from Cockles Obtained in Padang, Indonesia
title_full_unstemmed	Genotypic Characterization of Vibrio Sp. Isolated from Cockles Obtained in Padang, Indonesia
title_sort	genotypic characterization of vibrio sp. isolated from cockles obtained in padang, indonesia
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Biotechnology and Biomokular Sciences
publishDate	2006
url	http://psasir.upm.edu.my/id/eprint/5992/1/FBSB_2006_19.pdf
_version_	1776100244163919872

Genotypic Characterization of Vibrio Sp. Isolated from Cockles Obtained in Padang, Indonesia

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