Development and in vitro bioevaluation of cockle shell-calcium carbonate (Aragonite) nanoparticles for intracellular drug delivery

The use of safe and efficient delivery systems, capable of delivering therapeutic agents to subcellular levels are an ultimate goal in enhancing therapeutic effect. It is also a promising strategy in overcoming microbial resistance and the emergence of intracellular bacterial infections. The challe...

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Main Author:	Isa, Tijani
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Calcium carbonate Drug delivery systems
Online Access:	http://psasir.upm.edu.my/id/eprint/60455/1/IB%202015%203IR.pdf
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id	my-upm-ir.60455
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spelling	my-upm-ir.604552018-04-26T07:08:57Z Development and in vitro bioevaluation of cockle shell-calcium carbonate (Aragonite) nanoparticles for intracellular drug delivery 2015-10 Isa, Tijani The use of safe and efficient delivery systems, capable of delivering therapeutic agents to subcellular levels are an ultimate goal in enhancing therapeutic effect. It is also a promising strategy in overcoming microbial resistance and the emergence of intracellular bacterial infections. The challenge, however, is that the interaction of nanoparticles with biological systems at the cellular level must be established prior to biomedical applications. In this study,ciprofloxacin conjugated cockle shells-derived calcium carbonate (aragonite) nanoparticle (CCSCCAN) was developed and characterized for its physicochemical properties and antibacterial activities. Biocompatibilities were evaluated on macrophage cell line (J774.A1) using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and 5-Bromo-2ʹ-deoxyuridine (BrdU) assays. The nanoparticles were spherical in shape, with particles sizes ranging from 11.93 to 22.12 nm as determined through a transmission electron microscope (TEM). The highest percentage entrapment efficiency (EE) and loading content (LC) were 99.5% and 5.9%, respectively, with an optimum negative zeta potential. X-ray diffraction (XRD) patterns revealed strong crystallity of the formulations. Fourier transforms infrared (FT-IR) spectra shows evident of interactions exist between the drug and nanoparticles at the molecular level. No burst effect, but a sustained drug release was observed from the formulation. The mean diameter of inhibition zone was 18.6 ± 0.5 mm, which was better than ciprofloxacin alone (11.7 ± 0.9 mm), while the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the formulation were lower than those of free drugs. Study of biocompatability suggested non-toxic effects of the formulations. In conclusion, the results indicated that the ciprofloxacin- nanoparticle conjugate (C-CSCCAN) enhanced susceptibility of Salmonella and antibacterial efficacy of the antibiotic, which could potentially improve the clinical efficacy of the drug. Calcium carbonate Drug delivery systems 2015-10 Thesis http://psasir.upm.edu.my/id/eprint/60455/ http://psasir.upm.edu.my/id/eprint/60455/1/IB%202015%203IR.pdf text en public masters Universiti Putra Malaysia Calcium carbonate Drug delivery systems
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Calcium carbonate Drug delivery systems
spellingShingle	Calcium carbonate Drug delivery systems Isa, Tijani Development and in vitro bioevaluation of cockle shell-calcium carbonate (Aragonite) nanoparticles for intracellular drug delivery
description	The use of safe and efficient delivery systems, capable of delivering therapeutic agents to subcellular levels are an ultimate goal in enhancing therapeutic effect. It is also a promising strategy in overcoming microbial resistance and the emergence of intracellular bacterial infections. The challenge, however, is that the interaction of nanoparticles with biological systems at the cellular level must be established prior to biomedical applications. In this study,ciprofloxacin conjugated cockle shells-derived calcium carbonate (aragonite) nanoparticle (CCSCCAN) was developed and characterized for its physicochemical properties and antibacterial activities. Biocompatibilities were evaluated on macrophage cell line (J774.A1) using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and 5-Bromo-2ʹ-deoxyuridine (BrdU) assays. The nanoparticles were spherical in shape, with particles sizes ranging from 11.93 to 22.12 nm as determined through a transmission electron microscope (TEM). The highest percentage entrapment efficiency (EE) and loading content (LC) were 99.5% and 5.9%, respectively, with an optimum negative zeta potential. X-ray diffraction (XRD) patterns revealed strong crystallity of the formulations. Fourier transforms infrared (FT-IR) spectra shows evident of interactions exist between the drug and nanoparticles at the molecular level. No burst effect, but a sustained drug release was observed from the formulation. The mean diameter of inhibition zone was 18.6 ± 0.5 mm, which was better than ciprofloxacin alone (11.7 ± 0.9 mm), while the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the formulation were lower than those of free drugs. Study of biocompatability suggested non-toxic effects of the formulations. In conclusion, the results indicated that the ciprofloxacin- nanoparticle conjugate (C-CSCCAN) enhanced susceptibility of Salmonella and antibacterial efficacy of the antibiotic, which could potentially improve the clinical efficacy of the drug.
format	Thesis
qualification_level	Master's degree
author	Isa, Tijani
author_facet	Isa, Tijani
author_sort	Isa, Tijani
title	Development and in vitro bioevaluation of cockle shell-calcium carbonate (Aragonite) nanoparticles for intracellular drug delivery
title_short	Development and in vitro bioevaluation of cockle shell-calcium carbonate (Aragonite) nanoparticles for intracellular drug delivery
title_full	Development and in vitro bioevaluation of cockle shell-calcium carbonate (Aragonite) nanoparticles for intracellular drug delivery
title_fullStr	Development and in vitro bioevaluation of cockle shell-calcium carbonate (Aragonite) nanoparticles for intracellular drug delivery
title_full_unstemmed	Development and in vitro bioevaluation of cockle shell-calcium carbonate (Aragonite) nanoparticles for intracellular drug delivery
title_sort	development and in vitro bioevaluation of cockle shell-calcium carbonate (aragonite) nanoparticles for intracellular drug delivery
granting_institution	Universiti Putra Malaysia
publishDate	2015
url	http://psasir.upm.edu.my/id/eprint/60455/1/IB%202015%203IR.pdf
_version_	1747812273614749696

Development and in vitro bioevaluation of cockle shell-calcium carbonate (Aragonite) nanoparticles for intracellular drug delivery

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