A Comparative Study on the Molecular Characterization of E. Coli 0157 Isolated from Imported Beef in Malaysia and in Local and Imported Lamb in United Arab Emirates
This study was conducted to determine the incidence of E. coli 0157 in beef in Malaysia and lamb in United Arab Emirates. A total of 113 samples of beef (n = 25) and lamb (n = 88) were examined for the presence of E. coli 0157 which yielded 130 positive isolates for E. coli 01 57. All the isolate...
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| Format: | Thesis |
| Language: | English English |
| Published: |
2004
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/6287/1/FPSK%28M%29_2004_4.pdf |
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| Summary: | This study was conducted to determine the incidence of E. coli 0157 in beef in Malaysia
and lamb in United Arab Emirates. A total of 113 samples of beef (n = 25) and lamb (n =
88) were examined for the presence of E. coli 0157 which yielded 130 positive isolates
for E. coli 01 57. All the isolates were found to be resistant to one or more of the fifteen
antimicrobial agents tested. The strains isolated from lamb samples showed a high
occurrence in multiple drug resistance. Plasmid analysis showed that most of the E. coli
strains isolated from beef samples in Malaysia contained plasmids with size ranging from
1.3 to 60 MDa. The commonest plasmids of 60 MDa were observed in 3 1.8% of the
strains. On the other hand, representative patterns for plasmid profiles among
Esclzerichia coli O157 isolates from lamb possessed plasmid with molecular size ranging
from 1.2 to 35.8 MDa. However, there did not appear to be a consistent relationship
between a particular plasmid and resistance 10 an antibiotic. Two types of specific primer
encoding the Shiga-like toxins gene, the SL7' I and SL7' 11 gene were utilized in the
multiplex PCR assay. Analysis carried out demonstrated that Escherichia coli 0157
strains were positive for the presence sf Shiga-like toxins (stx) genes. Thus, analysis of
all the /:: coli genome showed that mqst of isolates carried either both SIJT I and SI,T II
genes or only the SI,T II gene. RAPD-PCR was used to generate polymorphic genomic
fingerprints to discriminate the E. coli 0157 isolates. After a set of ten-mer
oligonucleotides of 50% G-tC contents were screened, primers Gen1-50-9
(5 'AGAAGCGATG 3 ') and Genl -50,l 0 (S'CCATTTAC~ 3 ') were chosen whereby
the primers generated reproducible and typeable results with bands ranging from 0.25 to
4.0 kilobase pairs. All the dendrogram were constructed utilizing the Gel Compar and
RAPDistance software package based on the data retrieved from the presence or absence
of banding pattern. Based on the dendrogram generated, there appears to be a genetic
similarity at 70% among the strains issolated from beef and lamb. Two main groups were
observed at 10% similarity level, group ane consists of E. coli 0157 isolates from
imported beef in Malaysia and a few gf isolates of E. coli 0 157 from imported lamb in
UAE. On the other hand, group two consists mainly of eight isolates of E. coli 01 57 from
imported lamb in UAE. In the present study, all the molecular typing techniques have
been used generally provided an additional value for assisting the molecular
characterization of isolates of Escheric(ziu coli 0 157 utilized. |
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