Development Of Gene Deleted Recombinant Pseudorabies Virus

Development Of Gene Deleted Recombinant Pseudorabies Virus

The glycoprotein E (gE) and thymidine kinase (TK) genes are virulence-associated genes of pseudorabies virus (PrV). The study conducted was to shut down the gE gene from an established local TK defective (TK¯) PrV strain (TK¯gE+PrV). The ultimate aim of the study was to develop a gene-deleted recomb...

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Main Author: Alauddin, Zeenathul Nazariah
Format: Thesis
Language:English
English
Published: 2004
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Online Access:http://psasir.upm.edu.my/id/eprint/6418/1/ABSTRACT__FPV_2004_4.pdf
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spelling my-upm-ir.64182013-05-27T07:29:23Z Development Of Gene Deleted Recombinant Pseudorabies Virus 2004 Alauddin, Zeenathul Nazariah The glycoprotein E (gE) and thymidine kinase (TK) genes are virulence-associated genes of pseudorabies virus (PrV). The study conducted was to shut down the gE gene from an established local TK defective (TK¯) PrV strain (TK¯gE+PrV). The ultimate aim of the study was to develop a gene-deleted recombinant PrV with useful identification markers. A gE gene-deleted pseudorabies virus (TK¯gE¯PrV) was constructed by homologous recombinational techniques. The TK¯gE+PrV, regarded as the parental strain in the study, originated from a virulent local PrV isolate (TK+gE+PrV). Prior to the construction of the TK¯gE¯PrV, the gE of the parental strain was amplified, cloned and studied. Comparative sequence analysis showed that the gE sequence of TK¯gE+PrV was closely identical (98 %) to a Chinese Ea strain. The 10 nucleotide variations at nucleotide positions 237, 931, 1207, 1409, 1501, 1530, 1549, 1555, 1682 and 1842, led to six amino acids substitutions at amino acid residues 403 (A 2004 Thesis http://psasir.upm.edu.my/id/eprint/6418/ http://psasir.upm.edu.my/id/eprint/6418/1/ABSTRACT__FPV_2004_4.pdf application/pdf en public phd doctoral Universiti Putra Malaysia Faculty Veterinary Medicine English
institution Universiti Putra Malaysia
collection PSAS Institutional Repository
language English
English
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Alauddin, Zeenathul Nazariah
Development Of Gene Deleted Recombinant Pseudorabies Virus
description The glycoprotein E (gE) and thymidine kinase (TK) genes are virulence-associated genes of pseudorabies virus (PrV). The study conducted was to shut down the gE gene from an established local TK defective (TK¯) PrV strain (TK¯gE+PrV). The ultimate aim of the study was to develop a gene-deleted recombinant PrV with useful identification markers. A gE gene-deleted pseudorabies virus (TK¯gE¯PrV) was constructed by homologous recombinational techniques. The TK¯gE+PrV, regarded as the parental strain in the study, originated from a virulent local PrV isolate (TK+gE+PrV). Prior to the construction of the TK¯gE¯PrV, the gE of the parental strain was amplified, cloned and studied. Comparative sequence analysis showed that the gE sequence of TK¯gE+PrV was closely identical (98 %) to a Chinese Ea strain. The 10 nucleotide variations at nucleotide positions 237, 931, 1207, 1409, 1501, 1530, 1549, 1555, 1682 and 1842, led to six amino acids substitutions at amino acid residues 403 (A
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Alauddin, Zeenathul Nazariah
author_facet Alauddin, Zeenathul Nazariah
author_sort Alauddin, Zeenathul Nazariah
title Development Of Gene Deleted Recombinant Pseudorabies Virus
title_short Development Of Gene Deleted Recombinant Pseudorabies Virus
title_full Development Of Gene Deleted Recombinant Pseudorabies Virus
title_fullStr Development Of Gene Deleted Recombinant Pseudorabies Virus
title_full_unstemmed Development Of Gene Deleted Recombinant Pseudorabies Virus
title_sort development of gene deleted recombinant pseudorabies virus
granting_institution Universiti Putra Malaysia
granting_department Faculty Veterinary Medicine
publishDate 2004
url http://psasir.upm.edu.my/id/eprint/6418/1/ABSTRACT__FPV_2004_4.pdf
_version_ 1747810607615180800

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