Enzymatic hydrolysis of lignocellulosis biomass using extract from the termite Coptotermes curvignathus holmgren

Termites are by far the most successful wood-degraders on Earth, tunnelling and chewing on woody biomass for millions of years. To disintegrate the tough linear chains of cellulose, termites are loaded with different species of microorganisms in their relatively tiny guts. These gut microbes would c...

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Main Author:	Wong, Sin-Yee
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Termites - Physiology Cellulose - Biodegradation
Online Access:	http://psasir.upm.edu.my/id/eprint/66553/1/FH%202015%2010%20IR.pdf
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spelling	my-upm-ir.665532019-01-24T07:27:02Z Enzymatic hydrolysis of lignocellulosis biomass using extract from the termite Coptotermes curvignathus holmgren 2015-08 Wong, Sin-Yee Termites are by far the most successful wood-degraders on Earth, tunnelling and chewing on woody biomass for millions of years. To disintegrate the tough linear chains of cellulose, termites are loaded with different species of microorganisms in their relatively tiny guts. These gut microbes would collaborate to produce digestive enzymatic juice for degrading wood into consumable end products such as sugars, hydrogen, ethanol and acetate. By efficiently catalysing the conversion of lignocellulosic biomass, the key to generate customised cocktails lies within the termites’ digestive enzymes. This study aimed to identify and evaluate the cellulolytic enzymes activities in the whole extracts of local wood-feeding termites (Coptotermes curvignathus) for enzymatic hydrolysis with biomass such as oil palm trunk (OPT) and cassava pomace. The enzymatic hydrolysis was controlled at three different sets of temperature (27˚C ± 2˚C, 32˚C ± 2˚C and 37˚C ± 2˚C) and evaluated based on reaction time (hours). Conversion of biomass was measured in reducing sugar yield and cellulase activities of the reaction. Ultimately the results showed that the fresh extracts of termite Coptotermes curvignathus contained reducing sugar and activities of endo-β- D-1,4-glucanase, exo-β-D-1,4-glucanase and β-D-1,4-xylanase that could potentially increase the digestion of lignocellulosic biomass. The highest reducing sugar recorded was 7.36 ± 0.65 g/L in the enzymatic hydrolysis with OPT occurring at the reaction temperature of 37˚C, while the highest enzyme activities recorded were endocellulase (31.58 ± 5.48 U/g) in incubated termite extract, exocellulase (14.94 ± 4.71 U/g) in hydrolysis with OPT, and xylanase (89.60 ± 20.87 U/g) in hydrolysis with cassava, all occurring at the incubation temperature of 32˚C. Termites - Physiology Cellulose - Biodegradation 2015-08 Thesis http://psasir.upm.edu.my/id/eprint/66553/ http://psasir.upm.edu.my/id/eprint/66553/1/FH%202015%2010%20IR.pdf text en public masters Universiti Putra Malaysia Termites - Physiology Cellulose - Biodegradation
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Termites - Physiology Cellulose - Biodegradation
spellingShingle	Termites - Physiology Cellulose - Biodegradation Wong, Sin-Yee Enzymatic hydrolysis of lignocellulosis biomass using extract from the termite Coptotermes curvignathus holmgren
description	Termites are by far the most successful wood-degraders on Earth, tunnelling and chewing on woody biomass for millions of years. To disintegrate the tough linear chains of cellulose, termites are loaded with different species of microorganisms in their relatively tiny guts. These gut microbes would collaborate to produce digestive enzymatic juice for degrading wood into consumable end products such as sugars, hydrogen, ethanol and acetate. By efficiently catalysing the conversion of lignocellulosic biomass, the key to generate customised cocktails lies within the termites’ digestive enzymes. This study aimed to identify and evaluate the cellulolytic enzymes activities in the whole extracts of local wood-feeding termites (Coptotermes curvignathus) for enzymatic hydrolysis with biomass such as oil palm trunk (OPT) and cassava pomace. The enzymatic hydrolysis was controlled at three different sets of temperature (27˚C ± 2˚C, 32˚C ± 2˚C and 37˚C ± 2˚C) and evaluated based on reaction time (hours). Conversion of biomass was measured in reducing sugar yield and cellulase activities of the reaction. Ultimately the results showed that the fresh extracts of termite Coptotermes curvignathus contained reducing sugar and activities of endo-β- D-1,4-glucanase, exo-β-D-1,4-glucanase and β-D-1,4-xylanase that could potentially increase the digestion of lignocellulosic biomass. The highest reducing sugar recorded was 7.36 ± 0.65 g/L in the enzymatic hydrolysis with OPT occurring at the reaction temperature of 37˚C, while the highest enzyme activities recorded were endocellulase (31.58 ± 5.48 U/g) in incubated termite extract, exocellulase (14.94 ± 4.71 U/g) in hydrolysis with OPT, and xylanase (89.60 ± 20.87 U/g) in hydrolysis with cassava, all occurring at the incubation temperature of 32˚C.
format	Thesis
qualification_level	Master's degree
author	Wong, Sin-Yee
author_facet	Wong, Sin-Yee
author_sort	Wong, Sin-Yee
title	Enzymatic hydrolysis of lignocellulosis biomass using extract from the termite Coptotermes curvignathus holmgren
title_short	Enzymatic hydrolysis of lignocellulosis biomass using extract from the termite Coptotermes curvignathus holmgren
title_full	Enzymatic hydrolysis of lignocellulosis biomass using extract from the termite Coptotermes curvignathus holmgren
title_fullStr	Enzymatic hydrolysis of lignocellulosis biomass using extract from the termite Coptotermes curvignathus holmgren
title_full_unstemmed	Enzymatic hydrolysis of lignocellulosis biomass using extract from the termite Coptotermes curvignathus holmgren
title_sort	enzymatic hydrolysis of lignocellulosis biomass using extract from the termite coptotermes curvignathus holmgren
granting_institution	Universiti Putra Malaysia
publishDate	2015
url	http://psasir.upm.edu.my/id/eprint/66553/1/FH%202015%2010%20IR.pdf
_version_	1747812389505466368

Enzymatic hydrolysis of lignocellulosis biomass using extract from the termite Coptotermes curvignathus holmgren

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