Phytocomponents, safety, antioxidant, anti-inflammatory, hypocholesterolemic and anti-atherosclerotic properties of Basella alba L. leaf extract

Hypercholesterolemia is the major risk factor that leads to atherosclerosis, which is the primary cause of death in world population. Nowadays, alternative treatment using medicinal plants gained much attention since the treatment using synthetic drug, statins, lead to adverse health effects, especi...

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Main Author:	Gunasekaran, Baskaran
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Basellaceae - Therapeutic use Hypocholesteremia - Alternative treatment Plant extracts
Online Access:	http://psasir.upm.edu.my/id/eprint/66631/1/FBSB%202015%2023%20IR.pdf
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id	my-upm-ir.66631
record_format	uketd_dc
spelling	my-upm-ir.666312019-01-29T06:52:59Z Phytocomponents, safety, antioxidant, anti-inflammatory, hypocholesterolemic and anti-atherosclerotic properties of Basella alba L. leaf extract 2015-06 Gunasekaran, Baskaran Hypercholesterolemia is the major risk factor that leads to atherosclerosis, which is the primary cause of death in world population. Nowadays, alternative treatment using medicinal plants gained much attention since the treatment using synthetic drug, statins, lead to adverse health effects, especially liver and muscle toxicity. Thus, the focus of this study was on Basella alba leaf extract; its phytocomponents, toxicity, antioxidant, anti-inflammatory, hypocholesterolemic and anti-atherosclerotic properties. In this study, 25 medicinal plants extracts were screened for anti HMG-CoA reductase activity. B. alba leaf extract showed the highest inhibitory effect, about 74%. Therefore, B. alba was examined in order to investigate its phytochemical components. GC-MS/MS analysis detected 25 compounds while RP-HPLC revealed the presence of naringin, apigenin, luteolin, ascorbic acid and α-tocopherol. The toxicity evaluation of B. alba leaf extract using cytoxicity test against Vero and WRL-68 cell lines, acute and subchronic toxicity test in rats confirmed that B. alba extract is non toxic and can be utilized as alternative therapeutic agent. B. alba extract exhibits high antioxidant activity in inhibiting radicals like DPPH, nitric oxide and ferric ions. Anti-inhibitory activity of B. alba extract on hyaluronidase, xanthine oxidase and lipoxygenase enyzmes demonstrated a desirable anti-inflammatory activities.Twenty New Zealand white rabbits were divided into 5 groups and fed with normal diet, 2% high cholesterol diet (HCD), 2% HCD + 10 mg/kg simvastatin, 2% HCD + 100 mg/kg B. alba extract and 2% HCD + 200 mg/kg B. alba extract, respectively. The treatment with B. alba extract significantly lowered the elevated levels of TC, TG, LDL, livers enzymes (ALT and AST) and muscle enzyme (CK), and increased the level of HDL and antioxidant enzymes (SOD and GPx). B. alba extract significantly suppressed the aortic plaque formation. This is the first in vivo study on B. alba that suggests its potential as an alternative therapeutic agent for hypercholesterolemia and atherosclerosis. Basellaceae - Therapeutic use Hypocholesteremia - Alternative treatment Plant extracts 2015-06 Thesis http://psasir.upm.edu.my/id/eprint/66631/ http://psasir.upm.edu.my/id/eprint/66631/1/FBSB%202015%2023%20IR.pdf text en public doctoral Universiti Putra Malaysia Basellaceae - Therapeutic use Hypocholesteremia - Alternative treatment Plant extracts
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Basellaceae - Therapeutic use Hypocholesteremia - Alternative treatment Plant extracts
spellingShingle	Basellaceae - Therapeutic use Hypocholesteremia - Alternative treatment Plant extracts Gunasekaran, Baskaran Phytocomponents, safety, antioxidant, anti-inflammatory, hypocholesterolemic and anti-atherosclerotic properties of Basella alba L. leaf extract
description	Hypercholesterolemia is the major risk factor that leads to atherosclerosis, which is the primary cause of death in world population. Nowadays, alternative treatment using medicinal plants gained much attention since the treatment using synthetic drug, statins, lead to adverse health effects, especially liver and muscle toxicity. Thus, the focus of this study was on Basella alba leaf extract; its phytocomponents, toxicity, antioxidant, anti-inflammatory, hypocholesterolemic and anti-atherosclerotic properties. In this study, 25 medicinal plants extracts were screened for anti HMG-CoA reductase activity. B. alba leaf extract showed the highest inhibitory effect, about 74%. Therefore, B. alba was examined in order to investigate its phytochemical components. GC-MS/MS analysis detected 25 compounds while RP-HPLC revealed the presence of naringin, apigenin, luteolin, ascorbic acid and α-tocopherol. The toxicity evaluation of B. alba leaf extract using cytoxicity test against Vero and WRL-68 cell lines, acute and subchronic toxicity test in rats confirmed that B. alba extract is non toxic and can be utilized as alternative therapeutic agent. B. alba extract exhibits high antioxidant activity in inhibiting radicals like DPPH, nitric oxide and ferric ions. Anti-inhibitory activity of B. alba extract on hyaluronidase, xanthine oxidase and lipoxygenase enyzmes demonstrated a desirable anti-inflammatory activities.Twenty New Zealand white rabbits were divided into 5 groups and fed with normal diet, 2% high cholesterol diet (HCD), 2% HCD + 10 mg/kg simvastatin, 2% HCD + 100 mg/kg B. alba extract and 2% HCD + 200 mg/kg B. alba extract, respectively. The treatment with B. alba extract significantly lowered the elevated levels of TC, TG, LDL, livers enzymes (ALT and AST) and muscle enzyme (CK), and increased the level of HDL and antioxidant enzymes (SOD and GPx). B. alba extract significantly suppressed the aortic plaque formation. This is the first in vivo study on B. alba that suggests its potential as an alternative therapeutic agent for hypercholesterolemia and atherosclerosis.
format	Thesis
qualification_level	Doctorate
author	Gunasekaran, Baskaran
author_facet	Gunasekaran, Baskaran
author_sort	Gunasekaran, Baskaran
title	Phytocomponents, safety, antioxidant, anti-inflammatory, hypocholesterolemic and anti-atherosclerotic properties of Basella alba L. leaf extract
title_short	Phytocomponents, safety, antioxidant, anti-inflammatory, hypocholesterolemic and anti-atherosclerotic properties of Basella alba L. leaf extract
title_full	Phytocomponents, safety, antioxidant, anti-inflammatory, hypocholesterolemic and anti-atherosclerotic properties of Basella alba L. leaf extract
title_fullStr	Phytocomponents, safety, antioxidant, anti-inflammatory, hypocholesterolemic and anti-atherosclerotic properties of Basella alba L. leaf extract
title_full_unstemmed	Phytocomponents, safety, antioxidant, anti-inflammatory, hypocholesterolemic and anti-atherosclerotic properties of Basella alba L. leaf extract
title_sort	phytocomponents, safety, antioxidant, anti-inflammatory, hypocholesterolemic and anti-atherosclerotic properties of basella alba l. leaf extract
granting_institution	Universiti Putra Malaysia
publishDate	2015
url	http://psasir.upm.edu.my/id/eprint/66631/1/FBSB%202015%2023%20IR.pdf
_version_	1747812393446014976

Phytocomponents, safety, antioxidant, anti-inflammatory, hypocholesterolemic and anti-atherosclerotic properties of Basella alba L. leaf extract

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