Dysoxylum grande hiern as a potential source of biologically active constituents

Dysoxylum grande was chosen to be studied due to the lack of reported scientific investigations on the plant and interesting phytochemical findings on another plant of the same genus, Dysoxylum acutangulum. Two biological activities; antiacetylcholinesterase and ichthyotoxicity were used as the bioa...

Full description

Saved in:

Bibliographic Details
Main Author:	Low, Kok Wah
Format:	Thesis
Language:	English
Published:	2012
Subjects:	Botanical chemistry Plant bioactive compounds
Online Access:	http://psasir.upm.edu.my/id/eprint/66796/1/FS%202012%20100%20IR.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-upm-ir.66796
record_format	uketd_dc
spelling	my-upm-ir.667962019-02-01T00:21:31Z Dysoxylum grande hiern as a potential source of biologically active constituents 2012-06 Low, Kok Wah Dysoxylum grande was chosen to be studied due to the lack of reported scientific investigations on the plant and interesting phytochemical findings on another plant of the same genus, Dysoxylum acutangulum. Two biological activities; antiacetylcholinesterase and ichthyotoxicity were used as the bioassay in guiding the fractionation step. As a result, nine compounds were isolated from the ichthyotoxic EtOAc leaves fraction. Among them, eight were identified as new compounds named as 20S,24R-epoxy-7 ,25-dihydroxy-3,4-secodammar-4(28)-en-3-oic acid (CP1), grandol A (CP2), grandol B (CP3), grandol C (CP4), grandol D (CP5), grandol E (CP8), grandol F (CP9) and grandol G (CP10) and the known compound was identified as -sitosteryl- -D-glucopyranoside (CP11). All new compounds were identified based on thorough analysis of spectroscopic data including 1D and 2D NMR. The structure for the known compound was established by spectral data analyses and by comparison with reported investigations. In ichthyotoxic assay carried out on Zebra fish (Danio rerio), EtOAc fraction (leaves) was found to possess significant ichthyotoxicity compared to other fractions. However, the isolated pure compounds did not provide any IC50 values for the ichtyotoxicity test due to their limited amounts. All compounds were tested for anti-acetylcholinesterase activity using Thin Layer Chromatography (TLC)-bioautography with fast blue B salt. Only grandol A (CP2) and grandol B (CP3) showed positive results. However, the obtained IC50 for both of these compounds, grandol A (CP2) and B (CP3), using Ellman’s method was not significant (>200 μg/mL). Botanical chemistry Plant bioactive compounds 2012-06 Thesis http://psasir.upm.edu.my/id/eprint/66796/ http://psasir.upm.edu.my/id/eprint/66796/1/FS%202012%20100%20IR.pdf text en public masters Universiti Putra Malaysia Botanical chemistry Plant bioactive compounds
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Botanical chemistry Plant bioactive compounds
spellingShingle	Botanical chemistry Plant bioactive compounds Low, Kok Wah Dysoxylum grande hiern as a potential source of biologically active constituents
description	Dysoxylum grande was chosen to be studied due to the lack of reported scientific investigations on the plant and interesting phytochemical findings on another plant of the same genus, Dysoxylum acutangulum. Two biological activities; antiacetylcholinesterase and ichthyotoxicity were used as the bioassay in guiding the fractionation step. As a result, nine compounds were isolated from the ichthyotoxic EtOAc leaves fraction. Among them, eight were identified as new compounds named as 20S,24R-epoxy-7 ,25-dihydroxy-3,4-secodammar-4(28)-en-3-oic acid (CP1), grandol A (CP2), grandol B (CP3), grandol C (CP4), grandol D (CP5), grandol E (CP8), grandol F (CP9) and grandol G (CP10) and the known compound was identified as -sitosteryl- -D-glucopyranoside (CP11). All new compounds were identified based on thorough analysis of spectroscopic data including 1D and 2D NMR. The structure for the known compound was established by spectral data analyses and by comparison with reported investigations. In ichthyotoxic assay carried out on Zebra fish (Danio rerio), EtOAc fraction (leaves) was found to possess significant ichthyotoxicity compared to other fractions. However, the isolated pure compounds did not provide any IC50 values for the ichtyotoxicity test due to their limited amounts. All compounds were tested for anti-acetylcholinesterase activity using Thin Layer Chromatography (TLC)-bioautography with fast blue B salt. Only grandol A (CP2) and grandol B (CP3) showed positive results. However, the obtained IC50 for both of these compounds, grandol A (CP2) and B (CP3), using Ellman’s method was not significant (>200 μg/mL).
format	Thesis
qualification_level	Master's degree
author	Low, Kok Wah
author_facet	Low, Kok Wah
author_sort	Low, Kok Wah
title	Dysoxylum grande hiern as a potential source of biologically active constituents
title_short	Dysoxylum grande hiern as a potential source of biologically active constituents
title_full	Dysoxylum grande hiern as a potential source of biologically active constituents
title_fullStr	Dysoxylum grande hiern as a potential source of biologically active constituents
title_full_unstemmed	Dysoxylum grande hiern as a potential source of biologically active constituents
title_sort	dysoxylum grande hiern as a potential source of biologically active constituents
granting_institution	Universiti Putra Malaysia
publishDate	2012
url	http://psasir.upm.edu.my/id/eprint/66796/1/FS%202012%20100%20IR.pdf
_version_	1747812408028561408

Dysoxylum grande hiern as a potential source of biologically active constituents

Similar Items