Anti-hyperpermeability effect of Malaysian tualang honey on hydrogen peroxide-induced increased vascular permeability

Vascular hyperpermeability remains the main cause of underlying disorders in myocardial ischemia and atherosclerosis. Tualang Honey (TH) has been used in traditional medicine for decades and proven to possess multiple pharmacological actions. However to date, little is known about the use of T...

Full description

Saved in:

Bibliographic Details
Main Author:	Devasvaran, Kogilavanee
Format:	Thesis
Language:	English
Published:	2016
Subjects:	Medicine Traditional Capillary Permeability
Online Access:	http://psasir.upm.edu.my/id/eprint/66819/1/FPSK%20%28m%29%202016%2058%20IR.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-upm-ir.66819
record_format	uketd_dc
spelling	my-upm-ir.668192019-02-01T02:56:32Z Anti-hyperpermeability effect of Malaysian tualang honey on hydrogen peroxide-induced increased vascular permeability 2016-11 Devasvaran, Kogilavanee Vascular hyperpermeability remains the main cause of underlying disorders in myocardial ischemia and atherosclerosis. Tualang Honey (TH) has been used in traditional medicine for decades and proven to possess multiple pharmacological actions. However to date, little is known about the use of TH in anti‐inflammatory activity specifically in endothelial barrier protection. Thus, this study aimed to investigate the effects of TH on H2O2‐induced endothelial hyperpermeability in vitro and supported by an in vivo approach. In order to determine the effect of TH on endothelial hyperpermeability, HUVEC was pre‐treated with pre‐defined noncytotoxic concentration (via cytotoxicity assay) of TH for 4h and then exposed to 0.5mM H2O2. FITC‐dextran was used as a permeability indicator. The supportive in vivo study was the Miles assay, which quantified the extravasation of Evans blue dye in the dorsal skin of balb/c mice. To examine the cells morphological alterations, adherence junction proteins in HUVEC were identified using Fluorescein Phalloidin, caveolin-1 and β‐catenin immunofluorescence labeling. Intracellular calcium, PKC and cAMP signaling were also investigated. All data was analyzed using SPSS. LD50 of TH was found to be 3.7% and concentrations ranging from 0.01%‐1% showed no cytotoxic effect to HUVEC. Induction with H2O2 was found to increase HUVEC permeability but the effect was significantly reversed by TH (p<0.05), of which the permeability inhibition peaked at 0.1%. TH also significantly (p<0.05) reduced the effect of H2O2 in vivo in all concentrations in a dose-dependent manner. Immunofluorescence confirmed that TH reduced stress fiber formation and the colocalization of caveolin-1 and β-catenin in HUVEC. TH also significantly (p<0.05) decreased intracellular calcium release and PKC activity, while maintained the level of cAMP when induced with H2O2. In conclusions, TH ameliorates H2O2‐induced endothelial hyperpermeability in vitro and in vivo via suppression of adherence junction protein re‐distribution, reduction of intracellular calcium and PKC activity while maintaining the cAMP production. Medicine, Traditional Capillary Permeability 2016-11 Thesis http://psasir.upm.edu.my/id/eprint/66819/ http://psasir.upm.edu.my/id/eprint/66819/1/FPSK%20%28m%29%202016%2058%20IR.pdf text en public masters Universiti Putra Malaysia Medicine, Traditional Capillary Permeability
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Medicine Traditional Capillary Permeability
spellingShingle	Medicine Traditional Capillary Permeability Devasvaran, Kogilavanee Anti-hyperpermeability effect of Malaysian tualang honey on hydrogen peroxide-induced increased vascular permeability
description	Vascular hyperpermeability remains the main cause of underlying disorders in myocardial ischemia and atherosclerosis. Tualang Honey (TH) has been used in traditional medicine for decades and proven to possess multiple pharmacological actions. However to date, little is known about the use of TH in anti‐inflammatory activity specifically in endothelial barrier protection. Thus, this study aimed to investigate the effects of TH on H2O2‐induced endothelial hyperpermeability in vitro and supported by an in vivo approach. In order to determine the effect of TH on endothelial hyperpermeability, HUVEC was pre‐treated with pre‐defined noncytotoxic concentration (via cytotoxicity assay) of TH for 4h and then exposed to 0.5mM H2O2. FITC‐dextran was used as a permeability indicator. The supportive in vivo study was the Miles assay, which quantified the extravasation of Evans blue dye in the dorsal skin of balb/c mice. To examine the cells morphological alterations, adherence junction proteins in HUVEC were identified using Fluorescein Phalloidin, caveolin-1 and β‐catenin immunofluorescence labeling. Intracellular calcium, PKC and cAMP signaling were also investigated. All data was analyzed using SPSS. LD50 of TH was found to be 3.7% and concentrations ranging from 0.01%‐1% showed no cytotoxic effect to HUVEC. Induction with H2O2 was found to increase HUVEC permeability but the effect was significantly reversed by TH (p<0.05), of which the permeability inhibition peaked at 0.1%. TH also significantly (p<0.05) reduced the effect of H2O2 in vivo in all concentrations in a dose-dependent manner. Immunofluorescence confirmed that TH reduced stress fiber formation and the colocalization of caveolin-1 and β-catenin in HUVEC. TH also significantly (p<0.05) decreased intracellular calcium release and PKC activity, while maintained the level of cAMP when induced with H2O2. In conclusions, TH ameliorates H2O2‐induced endothelial hyperpermeability in vitro and in vivo via suppression of adherence junction protein re‐distribution, reduction of intracellular calcium and PKC activity while maintaining the cAMP production.
format	Thesis
qualification_level	Master's degree
author	Devasvaran, Kogilavanee
author_facet	Devasvaran, Kogilavanee
author_sort	Devasvaran, Kogilavanee
title	Anti-hyperpermeability effect of Malaysian tualang honey on hydrogen peroxide-induced increased vascular permeability
title_short	Anti-hyperpermeability effect of Malaysian tualang honey on hydrogen peroxide-induced increased vascular permeability
title_full	Anti-hyperpermeability effect of Malaysian tualang honey on hydrogen peroxide-induced increased vascular permeability
title_fullStr	Anti-hyperpermeability effect of Malaysian tualang honey on hydrogen peroxide-induced increased vascular permeability
title_full_unstemmed	Anti-hyperpermeability effect of Malaysian tualang honey on hydrogen peroxide-induced increased vascular permeability
title_sort	anti-hyperpermeability effect of malaysian tualang honey on hydrogen peroxide-induced increased vascular permeability
granting_institution	Universiti Putra Malaysia
publishDate	2016
url	http://psasir.upm.edu.my/id/eprint/66819/1/FPSK%20%28m%29%202016%2058%20IR.pdf
_version_	1747812411478376448

Anti-hyperpermeability effect of Malaysian tualang honey on hydrogen peroxide-induced increased vascular permeability

Similar Items