In vivo and in vitro studies of anti-aging properties of Dendrobium sabin flower

Exposure to UV radiation has induced reactive oxygen species (ROS) which enhanced the photo-induced aging of the skin characterized by wrinkles appearance. The antioxidant is one of the effective approaches to avoid from photo-induced aging problem. Dendrobium sabin flower was investigated for it...

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Main Author:	Abu, Farahziela
Format:	Thesis
Language:	English
Published:	2016
Subjects:	Skin Aging - physiology In Vitro Techniques
Online Access:	http://psasir.upm.edu.my/id/eprint/66957/1/FPSK%28m%29%202016%2055%20IR.pdf
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spelling	my-upm-ir.669572019-02-12T23:59:55Z In vivo and in vitro studies of anti-aging properties of Dendrobium sabin flower 2016-11 Abu, Farahziela Exposure to UV radiation has induced reactive oxygen species (ROS) which enhanced the photo-induced aging of the skin characterized by wrinkles appearance. The antioxidant is one of the effective approaches to avoid from photo-induced aging problem. Dendrobium sabin flower was investigated for its antioxidant and anti-aging properties. The oven-dried Dendrobium sabin flower was extracted using 100% methanol (w/v), 100% ethanol (w/v) and 100% water (w/v). The 100% methanolic crude extract showed the highest total phenolic content (40.33 ± mg GAE/g extract) and the best antioxidant properties as showed by DPPH, ABTS and FRAP assays. A correlation relationship between antioxidant activity and total phenolic content showed that phenolic compounds were the dominant antioxidant components in this flower extract. The microbial fermentation on Dendrobium sabin flower showed a potential in increasing the phenolic content and DPPH-scavenging activi ty. The TPC of final fermented medium showed the 18% increment while the DPPH of fermented medium increased significantly to approximately 80% a t the end of the fermentation. The treatment on brine shrimp and human dermal fibroblast primary cell using 100% methanolic crude extract demonstrated insignificant toxicity level. Topical application of 50 mg/ml of 100% methanolic crude extract showed improvement in epidermis and dermis thickness, which was comparable to the positive control treatment effect. The potential of the antioxidant and anti-aging properties found in Dendrobium sabin flower would help in treating undesirable photo-induced aging problems. Skin Aging - physiology In Vitro Techniques 2016-11 Thesis http://psasir.upm.edu.my/id/eprint/66957/ http://psasir.upm.edu.my/id/eprint/66957/1/FPSK%28m%29%202016%2055%20IR.pdf text en public masters Universiti Putra Malaysia Skin Aging - physiology In Vitro Techniques
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Skin Aging - physiology In Vitro Techniques
spellingShingle	Skin Aging - physiology In Vitro Techniques Abu, Farahziela In vivo and in vitro studies of anti-aging properties of Dendrobium sabin flower
description	Exposure to UV radiation has induced reactive oxygen species (ROS) which enhanced the photo-induced aging of the skin characterized by wrinkles appearance. The antioxidant is one of the effective approaches to avoid from photo-induced aging problem. Dendrobium sabin flower was investigated for its antioxidant and anti-aging properties. The oven-dried Dendrobium sabin flower was extracted using 100% methanol (w/v), 100% ethanol (w/v) and 100% water (w/v). The 100% methanolic crude extract showed the highest total phenolic content (40.33 ± mg GAE/g extract) and the best antioxidant properties as showed by DPPH, ABTS and FRAP assays. A correlation relationship between antioxidant activity and total phenolic content showed that phenolic compounds were the dominant antioxidant components in this flower extract. The microbial fermentation on Dendrobium sabin flower showed a potential in increasing the phenolic content and DPPH-scavenging activi ty. The TPC of final fermented medium showed the 18% increment while the DPPH of fermented medium increased significantly to approximately 80% a t the end of the fermentation. The treatment on brine shrimp and human dermal fibroblast primary cell using 100% methanolic crude extract demonstrated insignificant toxicity level. Topical application of 50 mg/ml of 100% methanolic crude extract showed improvement in epidermis and dermis thickness, which was comparable to the positive control treatment effect. The potential of the antioxidant and anti-aging properties found in Dendrobium sabin flower would help in treating undesirable photo-induced aging problems.
format	Thesis
qualification_level	Master's degree
author	Abu, Farahziela
author_facet	Abu, Farahziela
author_sort	Abu, Farahziela
title	In vivo and in vitro studies of anti-aging properties of Dendrobium sabin flower
title_short	In vivo and in vitro studies of anti-aging properties of Dendrobium sabin flower
title_full	In vivo and in vitro studies of anti-aging properties of Dendrobium sabin flower
title_fullStr	In vivo and in vitro studies of anti-aging properties of Dendrobium sabin flower
title_full_unstemmed	In vivo and in vitro studies of anti-aging properties of Dendrobium sabin flower
title_sort	in vivo and in vitro studies of anti-aging properties of dendrobium sabin flower
granting_institution	Universiti Putra Malaysia
publishDate	2016
url	http://psasir.upm.edu.my/id/eprint/66957/1/FPSK%28m%29%202016%2055%20IR.pdf
_version_	1747812429927022592

In vivo and in vitro studies of anti-aging properties of Dendrobium sabin flower

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