Evaluation of antidiabetic properties of Moringa oleifera Lam. leaves using In vitro model
Diabetes mellitus is a chronic condition affecting million of people worldwide including Malaysia. 347 million of people worldwide suffering diabetes and National Health and Morbidity Survey in 2011 showed that 20.8 % of Malaysian population suffering diabetes. Even though plenty of antidiabet...
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| Format: | Thesis |
| Language: | English |
| Published: |
2015
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/67690/1/fpsk%202015%2069%20IR.pdf |
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| Summary: | Diabetes mellitus is a chronic condition affecting million of people worldwide
including Malaysia. 347 million of people worldwide suffering diabetes and National
Health and Morbidity Survey in 2011 showed that 20.8 % of Malaysian population
suffering diabetes. Even though plenty of antidiabetic drug are available, it is still
remain the major worldwide health problems, which possibly due to the current drug
adverse side effect and its poor clinical efficacy. Continuous efforts are needed in
searching for new, safe and efficacious antidiabetic drug. Moringa oleifera is one of the
common medicinal plant uses in folk medicine. Traditionally, it has been claimed to
possess antidiabetic property. The study was carried out to authenticate the claimed
antidiabetic property and its possible mode of actions. In this study, antioxidant
capacity and antidiabetic evaluations on hot aqueous M. oleifera leaves extract using in
vitro model were done. Phytochemical study on M. oleifera indicated the presence of
polyphenols, with total phenolics content was found to be 3550 ± 100 mg gallic acid
equivalent per 100 g dry matter basis. It was shown from β-carotene bleaching assay
that M. oleifera preventing degradation of β-carotene by the peroxyl radicals and
exhibited 36% antioxidant capacity, whereas 2,2-diphenyl-2-picrylhydrazyl (DPPH)
radical scavenging assay exhibited that IC50 for hot aqueous M. oleifera was 0.32
mg/ml, suggesting that the extract potentially possesses free radical scavenging ability.
Toxicity evaluation by cell viability assay showed that M. oleifera did not cytotoxic to
BRIN-BD11, 3T3F442A adipocytes, L6 myotubes and Chang liver cells. Studies on
antidiabetic mechanism had shown that hot aqueous M. oleifera stimulated insulin
secretion from pancreatic β-cells significantly (p< 0.01). It had been found that
maximal insulin secretion ability of M. oleifera was 4.66 fold higher than
glibenclamide. M. oleifera enhanced basal and insulin-mediated glucose uptake into
adipocytes, muscles and liver cells. Insulin mimetic property was observed in
3T3F442A adipocytes cells whereas insulin sensitizing property and synergistic effect
with insulin property were observed in all cells tested. In vitro α-glucosidase inhibition
activity had shown that hot aqueous M. oleifera significantly inhibited rat intestine
sucrase activity with IC50 value of 977.24 μg/ml and exhibited competitive-type inhibition mechanism with Vmax value of 0.283 ± 0.003 mM/mg protein/min. This
study showed that M. oleifera possesses antidiabetic properties as claimed by the folk
medicine practitioners. Coupled with its antioxidant properties, it is suggested that the
plant has a potential to be developed as a new plant-derived oral antidiabetic agent. |
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