Modification of kosaric’s medium with nitrogen and phosphorus for Spirulina (Arthrospira platensis) in sheltered open culture system
Presently the world is experiencing serious environmental crisis such as global warming that effect the source of human nutrition hence, increasing starvation, and malnutrition. Demand for microalgae as supplemental food had increased steadily in recent years. However, in Malaysia, cultivation of...
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| Format: | Thesis |
| Language: | English |
| Published: |
2015
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/68130/1/FS%202015%2050%20IR.pdf |
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| Summary: | Presently the world is experiencing serious environmental crisis such as global
warming that effect the source of human nutrition hence, increasing starvation,
and malnutrition. Demand for microalgae as supplemental food had increased
steadily in recent years. However, in Malaysia, cultivation of microalgae is
scanty due to lack of knowledge in microalgae production in variable weather
conditions. One of the fundamental problems is lack of cheap and effective
fertilizer.
The aim of this research was to develop economical and inexpensive fertilizer
for a commercial production and give higher productivity of A. platensis. The
objective was implemented by substituting all the nitrogen and phosphorus
sources present in Kosaric Medium (SM) with locally available commercial
fertilizers. The A. platensis was treated with 19 treatments include SM as
control with three different nitrogen sources (ammonium nitrate, ammonium
sulphate, and urea) and three different phosphorus sources (phosphoric acids,
triple superphosphate (TSP), and diammonium phosphate (DAP)). Two grades
of nitrogen concentration representing 50% (high concentration) and 25% (low
concentration) of SM nitrogen concentration were compared in this study. 30L
A. platensis was grown in each polyethylene (PE) bag in outdoor condition for
seven days in first cultivation, ten days in second cultivation and 8 days in third
cultivation. The growth parameters (optical density, biomass and chlorophyll a
concentration) and biochemical content (proteins, carbohydrate and lipid) were
recorded and compared with A. platensis cultivated in SM.
Significant differences of the growth parameters and biochemical composition
were recorded for the different nitrogen and phosphorus sources and
concentration. The result of this study revealed that A. platensis cultivation
during dry season had better performance and A. platensis could utilize
ammonium nitrate (ammonium nitrate + triple superphosphate in low
concentration) most efficiently and gave the highest productivity on day 8 with biomass, chlorophyll, and protein yield of 1.24 ± 0.004g/L, 11.41 ± 0.21mg/l,
and 62.5%, respectively with average of pH, 10.12 ± 0.08 which was
significantly different (p<0.05) with other treatments. This was better than that
of SM (0.73± 0.01g/L, 8.64 ± 0.13mg/L, and 52%, respectively). Further
increased in nitrogen concentration will limit growth.
This study indicated that the utilization of ammonium nitrate and TSP as
nitrogen and phosphorus has the potential to replace the expensive Kosaric
media. The modified medium can be used cost effectively for large scale mass
production of protein rich A. platensis and yields similar performances to
Kosaric medium. |
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