Induction of periodontal disease via retentive ligature, lipopolysaccharide injection and their combination in a rat model

Periodontitis is a highly prevalent, chronic immune-inflammatory disease of the periodontium that results in progressive degradation of the periodontium and alveolar bone loss. This thesis aims to evaluate the induction of periodontal disease via retentive ligature, lipopolysaccharide, and their com...

Full description

Saved in:

Bibliographic Details
Main Author:	Mustafa, Hana Hassan
Format:	Thesis
Language:	English
Published:	2018
Subjects:	Periodontal disease Ligature (Surgery)
Online Access:	http://psasir.upm.edu.my/id/eprint/68612/1/FPV%202018%2012%20IR.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-upm-ir.68612
record_format	uketd_dc
spelling	my-upm-ir.686122019-05-21T07:59:44Z Induction of periodontal disease via retentive ligature, lipopolysaccharide injection and their combination in a rat model 2018-01 Mustafa, Hana Hassan Periodontitis is a highly prevalent, chronic immune-inflammatory disease of the periodontium that results in progressive degradation of the periodontium and alveolar bone loss. This thesis aims to evaluate the induction of periodontal disease via retentive ligature, lipopolysaccharide, and their combination in a rat model. Seventy two Sprague Dawley rats were distributed into four treatment groups: 1) control group with no treatment; 2) application of 4/0 nylon ligature around 2nd maxillary molars; 3) intragingival injection of Porphyromonas gingivalis lipopolysaccharide (LPS) to the palatal mucosa of the 2nd maxilla molars; 4) combination of ligature and LPS injection (ligature-LPS). At 7, 14, 30 days after the induction of periodontal disease, 6 rats of each group were sacrificed. Morphological changes in the gingival tissues were evaluated according to Loe & Sillness Gingival Index and Plaque Index. Alveolar bone loss were evaluated histologically and via microcomputed tomography. Parametric data were analysed using two-way ANOVA followed by Tukey test with significance set at 5%. Non-parametric data were analysed using Kruskal-Wallis followed by multiple comparisons with Bonferroni’s correction. The morphological, histological and radiological results revealed significant degenerative changes in the periodontal tissues and alveolar bone following both the ligature and ligature-LPS induction techniques. These changes were evident as early as 7 days; maintained until 14 days, and declined with time. There was minimal difference in the changes induced by ligature-LPS when compared to ligature alone. Injection with LPS alone resulted in minimal increase of the Gingival and Plaque Index, and insignificant histological and radiological changes when compared to the controls. In conclusions, the ligature technique was effective to induce acute periodontal disease. The LPS injection technique in this study was not effective to induce alveolar bone loss, and its combination to ligature added insignificant effect. Periodontal disease Ligature (Surgery) 2018-01 Thesis http://psasir.upm.edu.my/id/eprint/68612/ http://psasir.upm.edu.my/id/eprint/68612/1/FPV%202018%2012%20IR.pdf text en public masters Universiti Putra Malaysia Periodontal disease Ligature (Surgery)
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Periodontal disease Ligature (Surgery)
spellingShingle	Periodontal disease Ligature (Surgery) Mustafa, Hana Hassan Induction of periodontal disease via retentive ligature, lipopolysaccharide injection and their combination in a rat model
description	Periodontitis is a highly prevalent, chronic immune-inflammatory disease of the periodontium that results in progressive degradation of the periodontium and alveolar bone loss. This thesis aims to evaluate the induction of periodontal disease via retentive ligature, lipopolysaccharide, and their combination in a rat model. Seventy two Sprague Dawley rats were distributed into four treatment groups: 1) control group with no treatment; 2) application of 4/0 nylon ligature around 2nd maxillary molars; 3) intragingival injection of Porphyromonas gingivalis lipopolysaccharide (LPS) to the palatal mucosa of the 2nd maxilla molars; 4) combination of ligature and LPS injection (ligature-LPS). At 7, 14, 30 days after the induction of periodontal disease, 6 rats of each group were sacrificed. Morphological changes in the gingival tissues were evaluated according to Loe & Sillness Gingival Index and Plaque Index. Alveolar bone loss were evaluated histologically and via microcomputed tomography. Parametric data were analysed using two-way ANOVA followed by Tukey test with significance set at 5%. Non-parametric data were analysed using Kruskal-Wallis followed by multiple comparisons with Bonferroni’s correction. The morphological, histological and radiological results revealed significant degenerative changes in the periodontal tissues and alveolar bone following both the ligature and ligature-LPS induction techniques. These changes were evident as early as 7 days; maintained until 14 days, and declined with time. There was minimal difference in the changes induced by ligature-LPS when compared to ligature alone. Injection with LPS alone resulted in minimal increase of the Gingival and Plaque Index, and insignificant histological and radiological changes when compared to the controls. In conclusions, the ligature technique was effective to induce acute periodontal disease. The LPS injection technique in this study was not effective to induce alveolar bone loss, and its combination to ligature added insignificant effect.
format	Thesis
qualification_level	Master's degree
author	Mustafa, Hana Hassan
author_facet	Mustafa, Hana Hassan
author_sort	Mustafa, Hana Hassan
title	Induction of periodontal disease via retentive ligature, lipopolysaccharide injection and their combination in a rat model
title_short	Induction of periodontal disease via retentive ligature, lipopolysaccharide injection and their combination in a rat model
title_full	Induction of periodontal disease via retentive ligature, lipopolysaccharide injection and their combination in a rat model
title_fullStr	Induction of periodontal disease via retentive ligature, lipopolysaccharide injection and their combination in a rat model
title_full_unstemmed	Induction of periodontal disease via retentive ligature, lipopolysaccharide injection and their combination in a rat model
title_sort	induction of periodontal disease via retentive ligature, lipopolysaccharide injection and their combination in a rat model
granting_institution	Universiti Putra Malaysia
publishDate	2018
url	http://psasir.upm.edu.my/id/eprint/68612/1/FPV%202018%2012%20IR.pdf
_version_	1747812606202085376

Induction of periodontal disease via retentive ligature, lipopolysaccharide injection and their combination in a rat model

Similar Items