Effects of Polyphenols from Cocoa Powder on Diabetic Syndrome in Obese-Diabetic Rats
This study was carried out to determine the effects of polyphenols from cocoa powder on diabetic syndrome of obese-diabetic (Ob-db) rats. The studied diabetic syndrome are fasting plasma glucose level, oral glucose tolerance test, insulin level and insulin sensitivity, lipid profiles (total chole...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English English |
| Published: |
2007
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/7168/1/FPSK%28M%29_2009_6a.pdf |
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| Summary: | This study was carried out to determine the effects of polyphenols from cocoa powder on
diabetic syndrome of obese-diabetic (Ob-db) rats. The studied diabetic syndrome are fasting
plasma glucose level, oral glucose tolerance test, insulin level and insulin sensitivity, lipid
profiles (total cholesterol, triglycerides, high-density lipoprotein cholesterol, low-density
lipoprotein cholesterol, and free fatty acids), oxidative stress biomarker (8-isoprostane), and
antioxidant enzymes (superoxide dismutase and catalase). Obese-diabetic (Ob-db) rats were
developed using a high-fat diet (49% fat, 32% carbohydrate, and 19% protein from total
energy, kcal) for 3 months, followed by a low dose (35 mg/kg body weight) streptozotocin
(STZ) injection. Cocoa extract (600 mg/kg body weight) containing both polyphenols
(2.17mg epicatechin, 1.52 mg catechin, 0.25 mg dimer and 0.13 mg trimer g/cocoa extract)
and methylxanthines (3.55 mg caffeine and 2.22 mg theobromine g/cocoa extract) was given
to the rats for 4 weeks. The results indicated that there were no significant differences in fasting plasma glucose, insulin level and insulin sensitivity after 4 weeks of cocoa extracts
administration. Oral glucose tolerance test revealed that cocoa supplementation in Ob-db rats
significantly (p < 0.05) reduced plasma glucose at 60 and 90 min compared to
unsupplemented Ob-db rats. The supplementation significantly (p < 0.05) reduced the plasma
total cholesterol, triglycerides and low-density lipoprotein cholesterol of obese-diabetic rats
(Ob-db + cocoa) compared with non-supplemented animals (Ob-db). Plasma free fatty acid
and oxidative stress biomarker (8-isoprostane) were significantly (p < 0.05) reduced after
cocoa supplementation. Superoxide dismutase activity was enhanced in Ob-db compared to
that in non-supplemented rats. However, no change was observed in catalase activity. Cocoa
supplementation had an effect on postprandial glucose control but not for long-term glucose
control (4 weeks). Four weeks of cocoa extract supplementation also possess
hypocholesterolaemic properties. Moreover, cocoa supplementation could reduce circulating
plasma free fatty acid and 8 isoprostane and may enhance the antioxidant defense system. |
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