Effects of Polyphenols from Cocoa Powder on Diabetic Syndrome in Obese-Diabetic Rats

This study was carried out to determine the effects of polyphenols from cocoa powder on diabetic syndrome of obese-diabetic (Ob-db) rats. The studied diabetic syndrome are fasting plasma glucose level, oral glucose tolerance test, insulin level and insulin sensitivity, lipid profiles (total chole...

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Main Author:	Mhd Jalil, Abbe Maleyki
Format:	Thesis
Language:	English English
Published:	2007
Subjects:	Diabetes Mellitus
Online Access:	http://psasir.upm.edu.my/id/eprint/7168/1/FPSK%28M%29_2009_6a.pdf
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spelling	my-upm-ir.71682013-05-27T07:33:50Z Effects of Polyphenols from Cocoa Powder on Diabetic Syndrome in Obese-Diabetic Rats 2007 Mhd Jalil, Abbe Maleyki This study was carried out to determine the effects of polyphenols from cocoa powder on diabetic syndrome of obese-diabetic (Ob-db) rats. The studied diabetic syndrome are fasting plasma glucose level, oral glucose tolerance test, insulin level and insulin sensitivity, lipid profiles (total cholesterol, triglycerides, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, and free fatty acids), oxidative stress biomarker (8-isoprostane), and antioxidant enzymes (superoxide dismutase and catalase). Obese-diabetic (Ob-db) rats were developed using a high-fat diet (49% fat, 32% carbohydrate, and 19% protein from total energy, kcal) for 3 months, followed by a low dose (35 mg/kg body weight) streptozotocin (STZ) injection. Cocoa extract (600 mg/kg body weight) containing both polyphenols (2.17mg epicatechin, 1.52 mg catechin, 0.25 mg dimer and 0.13 mg trimer g/cocoa extract) and methylxanthines (3.55 mg caffeine and 2.22 mg theobromine g/cocoa extract) was given to the rats for 4 weeks. The results indicated that there were no significant differences in fasting plasma glucose, insulin level and insulin sensitivity after 4 weeks of cocoa extracts administration. Oral glucose tolerance test revealed that cocoa supplementation in Ob-db rats significantly (p < 0.05) reduced plasma glucose at 60 and 90 min compared to unsupplemented Ob-db rats. The supplementation significantly (p < 0.05) reduced the plasma total cholesterol, triglycerides and low-density lipoprotein cholesterol of obese-diabetic rats (Ob-db + cocoa) compared with non-supplemented animals (Ob-db). Plasma free fatty acid and oxidative stress biomarker (8-isoprostane) were significantly (p < 0.05) reduced after cocoa supplementation. Superoxide dismutase activity was enhanced in Ob-db compared to that in non-supplemented rats. However, no change was observed in catalase activity. Cocoa supplementation had an effect on postprandial glucose control but not for long-term glucose control (4 weeks). Four weeks of cocoa extract supplementation also possess hypocholesterolaemic properties. Moreover, cocoa supplementation could reduce circulating plasma free fatty acid and 8 isoprostane and may enhance the antioxidant defense system. Diabetes Mellitus 2007 Thesis http://psasir.upm.edu.my/id/eprint/7168/ http://psasir.upm.edu.my/id/eprint/7168/1/FPSK%28M%29_2009_6a.pdf application/pdf en public masters Universiti Putra Malaysia Diabetes Mellitus Medicine and Health Sciences English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Diabetes Mellitus
spellingShingle	Diabetes Mellitus Mhd Jalil, Abbe Maleyki Effects of Polyphenols from Cocoa Powder on Diabetic Syndrome in Obese-Diabetic Rats
description	This study was carried out to determine the effects of polyphenols from cocoa powder on diabetic syndrome of obese-diabetic (Ob-db) rats. The studied diabetic syndrome are fasting plasma glucose level, oral glucose tolerance test, insulin level and insulin sensitivity, lipid profiles (total cholesterol, triglycerides, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, and free fatty acids), oxidative stress biomarker (8-isoprostane), and antioxidant enzymes (superoxide dismutase and catalase). Obese-diabetic (Ob-db) rats were developed using a high-fat diet (49% fat, 32% carbohydrate, and 19% protein from total energy, kcal) for 3 months, followed by a low dose (35 mg/kg body weight) streptozotocin (STZ) injection. Cocoa extract (600 mg/kg body weight) containing both polyphenols (2.17mg epicatechin, 1.52 mg catechin, 0.25 mg dimer and 0.13 mg trimer g/cocoa extract) and methylxanthines (3.55 mg caffeine and 2.22 mg theobromine g/cocoa extract) was given to the rats for 4 weeks. The results indicated that there were no significant differences in fasting plasma glucose, insulin level and insulin sensitivity after 4 weeks of cocoa extracts administration. Oral glucose tolerance test revealed that cocoa supplementation in Ob-db rats significantly (p < 0.05) reduced plasma glucose at 60 and 90 min compared to unsupplemented Ob-db rats. The supplementation significantly (p < 0.05) reduced the plasma total cholesterol, triglycerides and low-density lipoprotein cholesterol of obese-diabetic rats (Ob-db + cocoa) compared with non-supplemented animals (Ob-db). Plasma free fatty acid and oxidative stress biomarker (8-isoprostane) were significantly (p < 0.05) reduced after cocoa supplementation. Superoxide dismutase activity was enhanced in Ob-db compared to that in non-supplemented rats. However, no change was observed in catalase activity. Cocoa supplementation had an effect on postprandial glucose control but not for long-term glucose control (4 weeks). Four weeks of cocoa extract supplementation also possess hypocholesterolaemic properties. Moreover, cocoa supplementation could reduce circulating plasma free fatty acid and 8 isoprostane and may enhance the antioxidant defense system.
format	Thesis
qualification_level	Master's degree
author	Mhd Jalil, Abbe Maleyki
author_facet	Mhd Jalil, Abbe Maleyki
author_sort	Mhd Jalil, Abbe Maleyki
title	Effects of Polyphenols from Cocoa Powder on Diabetic Syndrome in Obese-Diabetic Rats
title_short	Effects of Polyphenols from Cocoa Powder on Diabetic Syndrome in Obese-Diabetic Rats
title_full	Effects of Polyphenols from Cocoa Powder on Diabetic Syndrome in Obese-Diabetic Rats
title_fullStr	Effects of Polyphenols from Cocoa Powder on Diabetic Syndrome in Obese-Diabetic Rats
title_full_unstemmed	Effects of Polyphenols from Cocoa Powder on Diabetic Syndrome in Obese-Diabetic Rats
title_sort	effects of polyphenols from cocoa powder on diabetic syndrome in obese-diabetic rats
granting_institution	Universiti Putra Malaysia
granting_department	Medicine and Health Sciences
publishDate	2007
url	http://psasir.upm.edu.my/id/eprint/7168/1/FPSK%28M%29_2009_6a.pdf
_version_	1747810667077828608

Effects of Polyphenols from Cocoa Powder on Diabetic Syndrome in Obese-Diabetic Rats

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