Identification of mycosporinelike amino acids and 3-dehydroquinate synthase gene expression in UV radiation-induced Deinococcus radiodurans R1

Deinococcus radiodurans R1 is a well-known heterotrofic bacterium with extreme radio-resistant capability. It exhibits radiation survival up to 15,000 Gy while still be able to grow normally at 60 Gy/h. The radiation-tolerant mechanism that involves DNA repair represents 20% of the total resis...

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Main Author:	Huwaidi, Alaa Hassan Ibrahim Ahmed
Format:	Thesis
Language:	English
Published:	2018
Subjects:	Amino acids - Synthesis Genetic regulation Microbiological synthesis
Online Access:	http://psasir.upm.edu.my/id/eprint/75587/1/FBSB%202018%2018%20-%20IR.pdf
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id	my-upm-ir.75587
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spelling	my-upm-ir.755872019-11-27T01:45:10Z Identification of mycosporinelike amino acids and 3-dehydroquinate synthase gene expression in UV radiation-induced Deinococcus radiodurans R1 2018-07 Huwaidi, Alaa Hassan Ibrahim Ahmed Deinococcus radiodurans R1 is a well-known heterotrofic bacterium with extreme radio-resistant capability. It exhibits radiation survival up to 15,000 Gy while still be able to grow normally at 60 Gy/h. The radiation-tolerant mechanism that involves DNA repair represents 20% of the total resistant mechanism. Meanwhile, the other 80% comes from antioxidants. Since D. radiodurans R1 was discovered in 1956, the whole radio-resistant mechanism is not yet fully understood. Mycosporine-like amino acids (MAAs) are a group of 40 or more compounds that have antioxidant, growth stimulation and UV protective properties found in many microorganisms. In D. radiodurans R1, 3-dehydroquinate synthase (DHQ) gene annotated in chromosome 1 encodes the precursor for all MAAs. In this study, a significant amount of MAAs was found in D. radiodurans R1 after treatment with a different type of UV radiations, namely; UVA (360 nm) 6W and 100 W, and UVC (254 nm) 6W at a period of 12 to 48 hours. The RNA and MAAs were isolated from the UV-treated D. radiodurans R1. RT-qPCR experiment of the DHQ gene resulted in a significant increase in the number of expression fold from 4 to 9273 fold. Consequently, specific MAAs were identified using time-of-flight mass spectrometry (TOF-MS). They are mycosporinetaurine, mycosporine-glutamine, mycosporine-glutaminol, mycosporine-glutaminolglucoside, mycosoprine-glycine, mycosporine-2-glycine, mycosporineglycine: glutamic acid, shinorine, mycosporine-methylamine:serine, palythine-serine, and palythinol. The results suggested that these compounds play essential roles in D. radiodurans R1 radio-tolerance especially Mycosporine-methylamine:serine and palythine-serine for its expression at every UV treatment. This study may well help to understand radiation resistance mechanism further, and it is potential to be utilized as human protective compound against radiation risk. Amino acids - Synthesis Genetic regulation Microbiological synthesis 2018-07 Thesis http://psasir.upm.edu.my/id/eprint/75587/ http://psasir.upm.edu.my/id/eprint/75587/1/FBSB%202018%2018%20-%20IR.pdf text en public masters Universiti Putra Malaysia Amino acids - Synthesis Genetic regulation Microbiological synthesis
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Amino acids - Synthesis Genetic regulation Microbiological synthesis
spellingShingle	Amino acids - Synthesis Genetic regulation Microbiological synthesis Huwaidi, Alaa Hassan Ibrahim Ahmed Identification of mycosporinelike amino acids and 3-dehydroquinate synthase gene expression in UV radiation-induced Deinococcus radiodurans R1
description	Deinococcus radiodurans R1 is a well-known heterotrofic bacterium with extreme radio-resistant capability. It exhibits radiation survival up to 15,000 Gy while still be able to grow normally at 60 Gy/h. The radiation-tolerant mechanism that involves DNA repair represents 20% of the total resistant mechanism. Meanwhile, the other 80% comes from antioxidants. Since D. radiodurans R1 was discovered in 1956, the whole radio-resistant mechanism is not yet fully understood. Mycosporine-like amino acids (MAAs) are a group of 40 or more compounds that have antioxidant, growth stimulation and UV protective properties found in many microorganisms. In D. radiodurans R1, 3-dehydroquinate synthase (DHQ) gene annotated in chromosome 1 encodes the precursor for all MAAs. In this study, a significant amount of MAAs was found in D. radiodurans R1 after treatment with a different type of UV radiations, namely; UVA (360 nm) 6W and 100 W, and UVC (254 nm) 6W at a period of 12 to 48 hours. The RNA and MAAs were isolated from the UV-treated D. radiodurans R1. RT-qPCR experiment of the DHQ gene resulted in a significant increase in the number of expression fold from 4 to 9273 fold. Consequently, specific MAAs were identified using time-of-flight mass spectrometry (TOF-MS). They are mycosporinetaurine, mycosporine-glutamine, mycosporine-glutaminol, mycosporine-glutaminolglucoside, mycosoprine-glycine, mycosporine-2-glycine, mycosporineglycine: glutamic acid, shinorine, mycosporine-methylamine:serine, palythine-serine, and palythinol. The results suggested that these compounds play essential roles in D. radiodurans R1 radio-tolerance especially Mycosporine-methylamine:serine and palythine-serine for its expression at every UV treatment. This study may well help to understand radiation resistance mechanism further, and it is potential to be utilized as human protective compound against radiation risk.
format	Thesis
qualification_level	Master's degree
author	Huwaidi, Alaa Hassan Ibrahim Ahmed
author_facet	Huwaidi, Alaa Hassan Ibrahim Ahmed
author_sort	Huwaidi, Alaa Hassan Ibrahim Ahmed
title	Identification of mycosporinelike amino acids and 3-dehydroquinate synthase gene expression in UV radiation-induced Deinococcus radiodurans R1
title_short	Identification of mycosporinelike amino acids and 3-dehydroquinate synthase gene expression in UV radiation-induced Deinococcus radiodurans R1
title_full	Identification of mycosporinelike amino acids and 3-dehydroquinate synthase gene expression in UV radiation-induced Deinococcus radiodurans R1
title_fullStr	Identification of mycosporinelike amino acids and 3-dehydroquinate synthase gene expression in UV radiation-induced Deinococcus radiodurans R1
title_full_unstemmed	Identification of mycosporinelike amino acids and 3-dehydroquinate synthase gene expression in UV radiation-induced Deinococcus radiodurans R1
title_sort	identification of mycosporinelike amino acids and 3-dehydroquinate synthase gene expression in uv radiation-induced deinococcus radiodurans r1
granting_institution	Universiti Putra Malaysia
publishDate	2018
url	http://psasir.upm.edu.my/id/eprint/75587/1/FBSB%202018%2018%20-%20IR.pdf
_version_	1747813067598594048

Identification of mycosporinelike amino acids and 3-dehydroquinate synthase gene expression in UV radiation-induced Deinococcus radiodurans R1

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