Effects of drying methods on chemical composition, antioxidant and antibacterial activities of torch ginger (Etlingera elatior Jack) flower extracts and essential oil

Torch ginger (Etlingera elatior Jack) flower is a well-known spice for local delicacies and natural remedies that has been reported to possess significant antioxidant and antibacterial activities. However, the maturity stages, drying conditions and extraction methods are still questionable in pre...

Full description

Saved in:

Bibliographic Details
Main Author:	Anzian, Aliaa
Format:	Thesis
Language:	English
Published:	2018
Subjects:	Etlingera Antioxidants Antibacterial agents
Online Access:	http://psasir.upm.edu.my/id/eprint/77017/1/FSTM%202018%2017%20IR.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-upm-ir.77017
record_format	uketd_dc
spelling	my-upm-ir.770172020-02-11T02:41:16Z Effects of drying methods on chemical composition, antioxidant and antibacterial activities of torch ginger (Etlingera elatior Jack) flower extracts and essential oil 2018-04 Anzian, Aliaa Torch ginger (Etlingera elatior Jack) flower is a well-known spice for local delicacies and natural remedies that has been reported to possess significant antioxidant and antibacterial activities. However, the maturity stages, drying conditions and extraction methods are still questionable in preserving bioactive compounds of torch ginger flower. Therefore, the aims of this study were to profile the bioactive compounds in different maturity stages (tight bud and full bloom) of torch ginger flowers using Gas Chromatography-Mass spectrometry (GC-MS). Then, the effect of drying methods; freeze, oven and sun drying on extraction yield and antioxidant activities of selected maturity stage of torch ginger flowers were studied. Torch ginger flower’s essential oil extracted using subcritical carbon dioxide (SubC-CO2) were evaluated for antioxidant and antibacterial activities against selected foodborne pathogens, namely Bacillus cereus ATCC10876, Escherichia coli ATCC11229, Klebsiella pneumoniae ATCC13773, Listeria monocytogenes ATCC19111, Salmonella Typhimurium ATCC13311, and Staphylococcus aureus ATCC29213. Maturity stage had significant effect on percentage area of bioactive compounds concentrations (caryophyllene and 1- dodecanol) in torch ginger flower extract. Oven drying was identified as optimum extraction yield of 37.8±2.48% with high level of scavenging activity of 62.4±1.24% and IC50 value of 9.68 mg/mL, ferric reducing assay power of 2340.7±65.53 mg Fe2+/100 g, total phenols content of 331.6±2.85 mg GAE/100 g and total flavonoids of 465.8±2.08 mg QE/100 g. The antioxidant activities of torch ginger flower’s essential oil through DPPH assay (90.0±0.25%), FRAP assay= 8876.5±36.35, ABTS scavenging activity= 81.8±2.10%, TPC= 1590.2±3.74 mg GAE/100 g and TFC= 1686.2±17.34 mg QE/100 g. Minimal Inhibitory Concentration (MIC)= 1.56 mg/mL and Minimal Bactericidal Concentration (MBC)= 3.13 mg/mL of torch ginger flower’s essential oil were able to inhibit the growth of B. cereus and K. pneumoniae. This study clearly indicated that torch ginger flower could be potentially used as natural antioxidant and antibacterial agent in the food and pharmaceutical fields. Etlingera Antioxidants Antibacterial agents 2018-04 Thesis http://psasir.upm.edu.my/id/eprint/77017/ http://psasir.upm.edu.my/id/eprint/77017/1/FSTM%202018%2017%20IR.pdf text en public masters Universiti Putra Malaysia Etlingera Antioxidants Antibacterial agents Meor Hussin, Anis Shobirin
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
advisor	Meor Hussin, Anis Shobirin
topic	Etlingera Antioxidants Antibacterial agents
spellingShingle	Etlingera Antioxidants Antibacterial agents Anzian, Aliaa Effects of drying methods on chemical composition, antioxidant and antibacterial activities of torch ginger (Etlingera elatior Jack) flower extracts and essential oil
description	Torch ginger (Etlingera elatior Jack) flower is a well-known spice for local delicacies and natural remedies that has been reported to possess significant antioxidant and antibacterial activities. However, the maturity stages, drying conditions and extraction methods are still questionable in preserving bioactive compounds of torch ginger flower. Therefore, the aims of this study were to profile the bioactive compounds in different maturity stages (tight bud and full bloom) of torch ginger flowers using Gas Chromatography-Mass spectrometry (GC-MS). Then, the effect of drying methods; freeze, oven and sun drying on extraction yield and antioxidant activities of selected maturity stage of torch ginger flowers were studied. Torch ginger flower’s essential oil extracted using subcritical carbon dioxide (SubC-CO2) were evaluated for antioxidant and antibacterial activities against selected foodborne pathogens, namely Bacillus cereus ATCC10876, Escherichia coli ATCC11229, Klebsiella pneumoniae ATCC13773, Listeria monocytogenes ATCC19111, Salmonella Typhimurium ATCC13311, and Staphylococcus aureus ATCC29213. Maturity stage had significant effect on percentage area of bioactive compounds concentrations (caryophyllene and 1- dodecanol) in torch ginger flower extract. Oven drying was identified as optimum extraction yield of 37.8±2.48% with high level of scavenging activity of 62.4±1.24% and IC50 value of 9.68 mg/mL, ferric reducing assay power of 2340.7±65.53 mg Fe2+/100 g, total phenols content of 331.6±2.85 mg GAE/100 g and total flavonoids of 465.8±2.08 mg QE/100 g. The antioxidant activities of torch ginger flower’s essential oil through DPPH assay (90.0±0.25%), FRAP assay= 8876.5±36.35, ABTS scavenging activity= 81.8±2.10%, TPC= 1590.2±3.74 mg GAE/100 g and TFC= 1686.2±17.34 mg QE/100 g. Minimal Inhibitory Concentration (MIC)= 1.56 mg/mL and Minimal Bactericidal Concentration (MBC)= 3.13 mg/mL of torch ginger flower’s essential oil were able to inhibit the growth of B. cereus and K. pneumoniae. This study clearly indicated that torch ginger flower could be potentially used as natural antioxidant and antibacterial agent in the food and pharmaceutical fields.
format	Thesis
qualification_level	Master's degree
author	Anzian, Aliaa
author_facet	Anzian, Aliaa
author_sort	Anzian, Aliaa
title	Effects of drying methods on chemical composition, antioxidant and antibacterial activities of torch ginger (Etlingera elatior Jack) flower extracts and essential oil
title_short	Effects of drying methods on chemical composition, antioxidant and antibacterial activities of torch ginger (Etlingera elatior Jack) flower extracts and essential oil
title_full	Effects of drying methods on chemical composition, antioxidant and antibacterial activities of torch ginger (Etlingera elatior Jack) flower extracts and essential oil
title_fullStr	Effects of drying methods on chemical composition, antioxidant and antibacterial activities of torch ginger (Etlingera elatior Jack) flower extracts and essential oil
title_full_unstemmed	Effects of drying methods on chemical composition, antioxidant and antibacterial activities of torch ginger (Etlingera elatior Jack) flower extracts and essential oil
title_sort	effects of drying methods on chemical composition, antioxidant and antibacterial activities of torch ginger (etlingera elatior jack) flower extracts and essential oil
granting_institution	Universiti Putra Malaysia
publishDate	2018
url	http://psasir.upm.edu.my/id/eprint/77017/1/FSTM%202018%2017%20IR.pdf
_version_	1747813197842219008

Effects of drying methods on chemical composition, antioxidant and antibacterial activities of torch ginger (Etlingera elatior Jack) flower extracts and essential oil

Similar Items