Metabolomic profiling of antioxidant and anti-inflammatory properties in diatom Chaetoceros calcitrans extracts using NMR and UHPLC-MS coupled with chemometric analysis

Chaetoceros calcitrans is a diatom microalga that is known to be rich of amino acids, lipids, fatty acids and natural pigments identified as potentially important natural antioxidant and anti-inflammation. Nevertheless, little is known about the metabolome and the antioxidative and anti-inflammatory...

Full description

Saved in:

Bibliographic Details
Main Author:	Azizan, Awanis
Format:	Thesis
Language:	English
Published:	2019
Subjects:	Antioxidants Diatoms Microalgae
Online Access:	http://psasir.upm.edu.my/id/eprint/78480/1/IB%202019%2010%20ir.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-upm-ir.78480
record_format	uketd_dc
spelling	my-upm-ir.784802022-01-17T04:46:52Z Metabolomic profiling of antioxidant and anti-inflammatory properties in diatom Chaetoceros calcitrans extracts using NMR and UHPLC-MS coupled with chemometric analysis 2019-04 Azizan, Awanis Chaetoceros calcitrans is a diatom microalga that is known to be rich of amino acids, lipids, fatty acids and natural pigments identified as potentially important natural antioxidant and anti-inflammation. Nevertheless, little is known about the metabolome and the antioxidative and anti-inflammatory ability of the indigenous microalga, C. calcitrans. The main objectives of this study were to evaluate the metabolites that contributed to the antioxidant activity (DPPH*), nitric oxide (NO) inhibitory activity and total phenolic content (TPC) of C. calcitrans, extracted with different solvent polarities, including 70% ethanol, methanol, hexane, acetone, and chloroform using multi-platform metabolomics approaches. Nuclear magnetic resonance (NMR) coupled to multivariate data analysis (MVDA) was applied for the metabolomics profiling and relative quantification of the extracts. Further confirmation of the metabolites identification and quantitation were performed using ultra-high performance liquid chromatography mass spectrometry (UHPLC-MS). The results showed that acetone and chloroform (CHCl3) extracts of C. calcitrans revealed higher levels of TPC with 30.79 and 25.41 mg GAE/g dw, respectively. Both extracts also displayed moderate activity of DPPH radical scavenging inhibition with 43.01 and 35.03% at concentration 333 μg/ml. Furthermore, the CHCl3 extract inhibited the release of NO production from the LPS-activated RAW 264 cells with an IC50 value of 3.46 μg/ml. Twenty-nine metabolites were identified via NMR analyses from C. calcitrans extracts including 6 fatty acids, cholesterol, 11 amino acids, 2 sugars and 1 sugar-alcohol, 6 carotenoids and 2 chlorophylls. The structures of the compounds were also confirmed using tandem mass spectrometry. The main identified secondary metabolites were carotenoids including fucoxanthin, lutein, astaxanthin, canthaxanthin, zeaxanthin and violaxanthin. Comparison of different extracts revealed clear differences in the metabolite profiles and the partial least square (PLS) model indicated that the carotenoids were significantly associated with the tested bioactivities. The results suggested CHCl3 and acetone extracts of C. calcitrans showed the abundance of high-value metabolites as markers for antioxidant and anti inflammatory activities. The findings from this research may serve as a benchmark for future extraction processes particularly in recovering antioxidant and anti-inflammatory metabolites derived from diatom. These metabolites can be important active ingredients for medicinal preparation, functional foods, and cosmeceutical and nutraceutical applications. Antioxidants Diatoms Microalgae 2019-04 Thesis http://psasir.upm.edu.my/id/eprint/78480/ http://psasir.upm.edu.my/id/eprint/78480/1/IB%202019%2010%20ir.pdf text en public masters Universiti Putra Malaysia Antioxidants Diatoms Microalgae Abas, Faridah
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
advisor	Abas, Faridah
topic	Antioxidants Diatoms Microalgae
spellingShingle	Antioxidants Diatoms Microalgae Azizan, Awanis Metabolomic profiling of antioxidant and anti-inflammatory properties in diatom Chaetoceros calcitrans extracts using NMR and UHPLC-MS coupled with chemometric analysis
description	Chaetoceros calcitrans is a diatom microalga that is known to be rich of amino acids, lipids, fatty acids and natural pigments identified as potentially important natural antioxidant and anti-inflammation. Nevertheless, little is known about the metabolome and the antioxidative and anti-inflammatory ability of the indigenous microalga, C. calcitrans. The main objectives of this study were to evaluate the metabolites that contributed to the antioxidant activity (DPPH*), nitric oxide (NO) inhibitory activity and total phenolic content (TPC) of C. calcitrans, extracted with different solvent polarities, including 70% ethanol, methanol, hexane, acetone, and chloroform using multi-platform metabolomics approaches. Nuclear magnetic resonance (NMR) coupled to multivariate data analysis (MVDA) was applied for the metabolomics profiling and relative quantification of the extracts. Further confirmation of the metabolites identification and quantitation were performed using ultra-high performance liquid chromatography mass spectrometry (UHPLC-MS). The results showed that acetone and chloroform (CHCl3) extracts of C. calcitrans revealed higher levels of TPC with 30.79 and 25.41 mg GAE/g dw, respectively. Both extracts also displayed moderate activity of DPPH radical scavenging inhibition with 43.01 and 35.03% at concentration 333 μg/ml. Furthermore, the CHCl3 extract inhibited the release of NO production from the LPS-activated RAW 264 cells with an IC50 value of 3.46 μg/ml. Twenty-nine metabolites were identified via NMR analyses from C. calcitrans extracts including 6 fatty acids, cholesterol, 11 amino acids, 2 sugars and 1 sugar-alcohol, 6 carotenoids and 2 chlorophylls. The structures of the compounds were also confirmed using tandem mass spectrometry. The main identified secondary metabolites were carotenoids including fucoxanthin, lutein, astaxanthin, canthaxanthin, zeaxanthin and violaxanthin. Comparison of different extracts revealed clear differences in the metabolite profiles and the partial least square (PLS) model indicated that the carotenoids were significantly associated with the tested bioactivities. The results suggested CHCl3 and acetone extracts of C. calcitrans showed the abundance of high-value metabolites as markers for antioxidant and anti inflammatory activities. The findings from this research may serve as a benchmark for future extraction processes particularly in recovering antioxidant and anti-inflammatory metabolites derived from diatom. These metabolites can be important active ingredients for medicinal preparation, functional foods, and cosmeceutical and nutraceutical applications.
format	Thesis
qualification_level	Master's degree
author	Azizan, Awanis
author_facet	Azizan, Awanis
author_sort	Azizan, Awanis
title	Metabolomic profiling of antioxidant and anti-inflammatory properties in diatom Chaetoceros calcitrans extracts using NMR and UHPLC-MS coupled with chemometric analysis
title_short	Metabolomic profiling of antioxidant and anti-inflammatory properties in diatom Chaetoceros calcitrans extracts using NMR and UHPLC-MS coupled with chemometric analysis
title_full	Metabolomic profiling of antioxidant and anti-inflammatory properties in diatom Chaetoceros calcitrans extracts using NMR and UHPLC-MS coupled with chemometric analysis
title_fullStr	Metabolomic profiling of antioxidant and anti-inflammatory properties in diatom Chaetoceros calcitrans extracts using NMR and UHPLC-MS coupled with chemometric analysis
title_full_unstemmed	Metabolomic profiling of antioxidant and anti-inflammatory properties in diatom Chaetoceros calcitrans extracts using NMR and UHPLC-MS coupled with chemometric analysis
title_sort	metabolomic profiling of antioxidant and anti-inflammatory properties in diatom chaetoceros calcitrans extracts using nmr and uhplc-ms coupled with chemometric analysis
granting_institution	Universiti Putra Malaysia
publishDate	2019
url	http://psasir.upm.edu.my/id/eprint/78480/1/IB%202019%2010%20ir.pdf
_version_	1747813299532070912

Metabolomic profiling of antioxidant and anti-inflammatory properties in diatom Chaetoceros calcitrans extracts using NMR and UHPLC-MS coupled with chemometric analysis

Similar Items