Fermentation of Pineapple Taste Juice for the Production of Citric Acid Using Candida Lipolytica ATCC 8661
Fermentation of pineapple waste juice.using Candida lipolytica ATCC 8661 for production of citric acid was conducted. A comparison study was also carried out using the pineapple flesh juice (edible portion). Maximum production of citric acid obtained from the flesh juice and waste juice was 2.5...
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| Format: | Thesis |
| Language: | English English |
| Published: |
1990
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/8345/1/FSMB_1990_4_A.pdf |
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| Summary: | Fermentation of pineapple waste juice.using Candida lipolytica ATCC 8661 for production of citric acid was
conducted. A comparison study was also carried out using the
pineapple flesh juice (edible portion). Maximum production of
citric acid obtained from the flesh juice and waste juice was
2.5 g/l and 3.2 g/l respectively after 4 Days under submerged
fermentation using shake flasks at 30°C .The addition of 0.1% potassium dihydrogen phosphate in the
10Z pure glucose media increased the production of total acids
(citric acid). Addition of 3% methanol prior to incubation
into the pineapple waste juice showed a remarkable increase in citric acid production. Fermentation of pineapple waste juice
using Candida lipolytica ATCC 8661 was also conducted in LH
fermentor (Model 500). The effect of different levels of
dissolved oxygen saturation (10%, 20%, 30% and 40%) with and
without 3% methanol on citric acid production of pineapple
waste juice was studied over a 10-day fermentation period at
30C. The maximum citric acid production was 8.2 g/l in sample
with 3% methanol having 40% dissolved oxygen saturation after 8
days of fermentation. Maximum of biomass production of 12. 37
mg/ml juice (dry cell weight) was obtained from fermented
pineapple waste juice inoculated with C. lipolytica ATCC 8661
having 30% dissolved oxygen saturation after 8-10 days of
fermentation. |
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