Tissue Specific Localization of Several Oil Palm Genes During Flower Development

Flowering is the first introductory step to fruit formation and is a fundamental part of the plants reproduction system. Flowers and fruit are also an integral part of seed production. In most crops, the control of flowering is an important aspect of growth and development. If oil palm flowering...

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Main Author:	Rahmat, Zaidah
Format:	Thesis
Language:	English English
Published:	2001
Subjects:	Plants - Reproduction. Oil palm - Flowering. Plant cells and tissues.
Online Access:	http://psasir.upm.edu.my/id/eprint/8453/1/FSMB_2001_29_IR.pdf
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id	my-upm-ir.8453
record_format	uketd_dc
spelling	my-upm-ir.84532024-01-24T01:11:37Z Tissue Specific Localization of Several Oil Palm Genes During Flower Development 2001-07 Rahmat, Zaidah Flowering is the first introductory step to fruit formation and is a fundamental part of the plants reproduction system. Flowers and fruit are also an integral part of seed production. In most crops, the control of flowering is an important aspect of growth and development. If oil palm flowering could be controlled, yield could be improved by stimulating flowering in accordance to permissive environmental factors. In order to determine the factors that influence flowering of oil palm, the physiological background and the flowering process must be studied. However, oil palm micropropagation had come up against a major difficulty with the discovery of a floral morphogenesis abnormality induced by in vitro regeneration (Corley et al., 1986; Toruan-Mathius et al., 1998). From the examination of both morphology and anatomy of oil palm flower development, 9 key stages of normal and abnormal flower development has been classified to assist in the study of tissue specific expression of flowering genes. As plant organ systems are composed of anatomically similar cells and tissues, in situ hybridization was chosen as a method of determining gene expression based on its sensitivity and ability to determine the specific location of an mRNA. Examinations carried out on 4 oil palm flowering genes provide more information about the processes occurring during normal and abnormal flower formation of oil palm. OPSOC1, an oil palm homologue of AGL20 and OPLFY, the LFY homologue of oil palm, are both expressed throughout flower initiation and development. OPRLK5, a member of the receptor kinase gene family, is expressed throughout flower development. The last gene, OPUIP2, which encodes a UFO-interacting protein, is also expressed throughout flower development but it is not needed during inflorescence meristem development. Combined with other studies in this area, it is hoped that an understanding of the floral abnormality may be within reach in the near future. Attempts to isolate flower specific genes from an oil palm floral cDNA library however have been unsuccessful. The choice of cDNA library and conventional molecular tools might not be applicable in isolating these types of genes. Nevertheless, with advanced molecular and genetic tools such as yeast one and two-hybrid system that are being developed, isolation and the determination of function of such genes can be achieved. Plants - Reproduction. Oil palm - Flowering. Plant cells and tissues. 2001-07 Thesis http://psasir.upm.edu.my/id/eprint/8453/ http://psasir.upm.edu.my/id/eprint/8453/1/FSMB_2001_29_IR.pdf text en public masters Universiti Putra Malaysia Plants - Reproduction. Oil palm - Flowering. Plant cells and tissues. Food Science and Technology Harikrishna, K. English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Harikrishna, K.
topic	Plants - Reproduction. Oil palm - Flowering. Plant cells and tissues.
spellingShingle	Plants - Reproduction. Oil palm - Flowering. Plant cells and tissues. Rahmat, Zaidah Tissue Specific Localization of Several Oil Palm Genes During Flower Development
description	Flowering is the first introductory step to fruit formation and is a fundamental part of the plants reproduction system. Flowers and fruit are also an integral part of seed production. In most crops, the control of flowering is an important aspect of growth and development. If oil palm flowering could be controlled, yield could be improved by stimulating flowering in accordance to permissive environmental factors. In order to determine the factors that influence flowering of oil palm, the physiological background and the flowering process must be studied. However, oil palm micropropagation had come up against a major difficulty with the discovery of a floral morphogenesis abnormality induced by in vitro regeneration (Corley et al., 1986; Toruan-Mathius et al., 1998). From the examination of both morphology and anatomy of oil palm flower development, 9 key stages of normal and abnormal flower development has been classified to assist in the study of tissue specific expression of flowering genes. As plant organ systems are composed of anatomically similar cells and tissues, in situ hybridization was chosen as a method of determining gene expression based on its sensitivity and ability to determine the specific location of an mRNA. Examinations carried out on 4 oil palm flowering genes provide more information about the processes occurring during normal and abnormal flower formation of oil palm. OPSOC1, an oil palm homologue of AGL20 and OPLFY, the LFY homologue of oil palm, are both expressed throughout flower initiation and development. OPRLK5, a member of the receptor kinase gene family, is expressed throughout flower development. The last gene, OPUIP2, which encodes a UFO-interacting protein, is also expressed throughout flower development but it is not needed during inflorescence meristem development. Combined with other studies in this area, it is hoped that an understanding of the floral abnormality may be within reach in the near future. Attempts to isolate flower specific genes from an oil palm floral cDNA library however have been unsuccessful. The choice of cDNA library and conventional molecular tools might not be applicable in isolating these types of genes. Nevertheless, with advanced molecular and genetic tools such as yeast one and two-hybrid system that are being developed, isolation and the determination of function of such genes can be achieved.
format	Thesis
qualification_level	Master's degree
author	Rahmat, Zaidah
author_facet	Rahmat, Zaidah
author_sort	Rahmat, Zaidah
title	Tissue Specific Localization of Several Oil Palm Genes During Flower Development
title_short	Tissue Specific Localization of Several Oil Palm Genes During Flower Development
title_full	Tissue Specific Localization of Several Oil Palm Genes During Flower Development
title_fullStr	Tissue Specific Localization of Several Oil Palm Genes During Flower Development
title_full_unstemmed	Tissue Specific Localization of Several Oil Palm Genes During Flower Development
title_sort	tissue specific localization of several oil palm genes during flower development
granting_institution	Universiti Putra Malaysia
granting_department	Food Science and Technology
publishDate	2001
url	http://psasir.upm.edu.my/id/eprint/8453/1/FSMB_2001_29_IR.pdf
_version_	1794018752930512896

Tissue Specific Localization of Several Oil Palm Genes During Flower Development

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