Isolation and Characterisation of Thionin Genes in Rice (Oryza Sativa)

Thionins are cysteine rich, basic and toxic proteins that are assumed to be involved in the defense against pathogens. Two partial cDNA clones encoding for putative thionin in rice were isolated from endosperm (Rth-l) and shoots (Rth-2). The nucleotide sequences encoding for thionin precursor wer...

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Main Author:	Jason, Chiew
Format:	Thesis
Language:	English English
Published:	2001
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Online Access:	http://psasir.upm.edu.my/id/eprint/8461/1/FSMB_2001_37_IR.pdf
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id	my-upm-ir.8461
record_format	uketd_dc
spelling	my-upm-ir.84612024-01-26T01:48:51Z Isolation and Characterisation of Thionin Genes in Rice (Oryza Sativa) 2001-04 Jason, Chiew Thionins are cysteine rich, basic and toxic proteins that are assumed to be involved in the defense against pathogens. Two partial cDNA clones encoding for putative thionin in rice were isolated from endosperm (Rth-l) and shoots (Rth-2). The nucleotide sequences encoding for thionin precursor were further identified from the amplification of the 3' -end of the cDNA. The amino acid sequence for JRth-l isolated from endosperm showed 72% homology to barley leaf thionins DB4 (Bohlmann and Apel, 1987), 68% to pKG1940 (Gausing, 1987), 51% to hordothionin Hth-1 (Rodriguez-Palenzuela et 01., 1988), 50% to purothionin Pur-AI (Ohtani et 01., 1977), 33% to crambin Thi2Ca1 (Teeter et 01., 1981) and viscotoxin Vis A3 (Samuelsson et 01., 1974). The homologies for JRth-2 at the amino acid level were 83% to barley leaf thionins DB4 (Bohlmann and Apel, 1987), 71% to pKG1940 (Gausing, 1987), 46% to the hordothionin Hth-l (Rodriguez-Palenzuela et 01., 1988) and purothionin Pur-AI (Ohtani et 01., 1977), 41% to crambin Thi2CaI (Teeter et 01., 1981) and 33% to the viscotoxin Vis A3 (Samuelsson et 01., 1974). Like other thionin precursors, the putative rice thionin has an N-terminal domain characteristic of a signal peptide and an acidic C-terminal extension. In the expression studies, rice thionins mRNA were found to accumulate abundantly in endosperm and shoots only with a transcript size of around 600-700 bp. In both of these tissues, the expression of the thionin genes was strongly repressed by the exposure to light. Southern analysis showed that the rice thionin seemed to be coded by 1-2 genes. 2001-04 Thesis http://psasir.upm.edu.my/id/eprint/8461/ http://psasir.upm.edu.my/id/eprint/8461/1/FSMB_2001_37_IR.pdf text en public masters Universiti Putra Malaysia Food Science and Technology Napis, Suhaimi English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Napis, Suhaimi
topic
spellingShingle	Jason, Chiew Isolation and Characterisation of Thionin Genes in Rice (Oryza Sativa)
description	Thionins are cysteine rich, basic and toxic proteins that are assumed to be involved in the defense against pathogens. Two partial cDNA clones encoding for putative thionin in rice were isolated from endosperm (Rth-l) and shoots (Rth-2). The nucleotide sequences encoding for thionin precursor were further identified from the amplification of the 3' -end of the cDNA. The amino acid sequence for JRth-l isolated from endosperm showed 72% homology to barley leaf thionins DB4 (Bohlmann and Apel, 1987), 68% to pKG1940 (Gausing, 1987), 51% to hordothionin Hth-1 (Rodriguez-Palenzuela et 01., 1988), 50% to purothionin Pur-AI (Ohtani et 01., 1977), 33% to crambin Thi2Ca1 (Teeter et 01., 1981) and viscotoxin Vis A3 (Samuelsson et 01., 1974). The homologies for JRth-2 at the amino acid level were 83% to barley leaf thionins DB4 (Bohlmann and Apel, 1987), 71% to pKG1940 (Gausing, 1987), 46% to the hordothionin Hth-l (Rodriguez-Palenzuela et 01., 1988) and purothionin Pur-AI (Ohtani et 01., 1977), 41% to crambin Thi2CaI (Teeter et 01., 1981) and 33% to the viscotoxin Vis A3 (Samuelsson et 01., 1974). Like other thionin precursors, the putative rice thionin has an N-terminal domain characteristic of a signal peptide and an acidic C-terminal extension. In the expression studies, rice thionins mRNA were found to accumulate abundantly in endosperm and shoots only with a transcript size of around 600-700 bp. In both of these tissues, the expression of the thionin genes was strongly repressed by the exposure to light. Southern analysis showed that the rice thionin seemed to be coded by 1-2 genes.
format	Thesis
qualification_level	Master's degree
author	Jason, Chiew
author_facet	Jason, Chiew
author_sort	Jason, Chiew
title	Isolation and Characterisation of Thionin Genes in Rice (Oryza Sativa)
title_short	Isolation and Characterisation of Thionin Genes in Rice (Oryza Sativa)
title_full	Isolation and Characterisation of Thionin Genes in Rice (Oryza Sativa)
title_fullStr	Isolation and Characterisation of Thionin Genes in Rice (Oryza Sativa)
title_full_unstemmed	Isolation and Characterisation of Thionin Genes in Rice (Oryza Sativa)
title_sort	isolation and characterisation of thionin genes in rice (oryza sativa)
granting_institution	Universiti Putra Malaysia
granting_department	Food Science and Technology
publishDate	2001
url	http://psasir.upm.edu.my/id/eprint/8461/1/FSMB_2001_37_IR.pdf
_version_	1794018754614525952

Isolation and Characterisation of Thionin Genes in Rice (Oryza Sativa)

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