Characterization of Lipase Produced by Thermophilic Fungi Isolated From Pome
A plate assay to determine lipase activity was developed. Tween 80, used as a substrate with Victoria Blue B as the indicator seemed to be a reliable technique. Lipolytic activity was determined by the formation of the zone of intensification of the indicator colour after 24 hr. Intensity of the...
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1990
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my-upm-ir.85612012-05-07T04:31:46Z Characterization of Lipase Produced by Thermophilic Fungi Isolated From Pome 1990 Abd. Samad, Mohd. Yusoff A plate assay to determine lipase activity was developed. Tween 80, used as a substrate with Victoria Blue B as the indicator seemed to be a reliable technique. Lipolytic activity was determined by the formation of the zone of intensification of the indicator colour after 24 hr. Intensity of the colour developed was greater than that of triolein dye system and clearer than the hydrolysis zone of tributyrin plate. Test using a commercial enzyme and growth media containing lipolytic activity showed that the zone of intensification increased with increased lipolytic activity. A linear relationship could be seen when log enzyme concentration was plotted against the diameter of zone of intensification. Using this technique primary screening of lipolytic microorganisms could be conducted using the formation of zone of intensification around the colonies and mycelia. Five fungi showing lipolytic activity on plate were isolated using plate assay. However, using broth culture only one fungus was found to produce lipase. The fungus was identified as Rhizopusrhizopodiformis, (strain S1 and S2). Strain S1 was found to produce maximum lipase yield in the basal medium containing of peptone and glycerol. Lipase - Case studies Thermophilic fungi 1990 Thesis http://psasir.upm.edu.my/id/eprint/8561/ http://psasir.upm.edu.my/id/eprint/8561/1/FSAS_1990_4_A.pdf application/pdf en public masters Universiti Pertanian Malaysia Lipase - Case studies Thermophilic fungi Faculty of Environmental Studies English |
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Universiti Putra Malaysia |
| collection |
PSAS Institutional Repository |
| language |
English English |
| topic |
Lipase - Case studies Thermophilic fungi |
| spellingShingle |
Lipase - Case studies Thermophilic fungi Abd. Samad, Mohd. Yusoff Characterization of Lipase Produced by Thermophilic Fungi Isolated From Pome |
| description |
A plate assay to determine lipase activity was developed.
Tween 80, used as a substrate with Victoria Blue B as the
indicator seemed to be a reliable technique. Lipolytic activity
was determined by the formation of the zone of intensification of
the indicator colour after 24 hr. Intensity of the colour
developed was greater than that of triolein dye system and
clearer than the hydrolysis zone of tributyrin plate. Test using
a commercial enzyme and growth media containing lipolytic
activity showed that the zone of intensification increased with
increased lipolytic activity. A linear relationship could be
seen when log enzyme concentration was plotted against the
diameter of zone of intensification. Using this technique primary screening of lipolytic microorganisms could be conducted using
the formation of zone of intensification around the colonies and
mycelia. Five fungi showing lipolytic activity on plate were isolated
using plate assay. However, using broth culture only one fungus
was found to produce lipase. The fungus was identified as
Rhizopusrhizopodiformis, (strain S1 and S2). Strain S1 was found
to produce maximum lipase yield in the basal medium containing of
peptone and glycerol. |
| format |
Thesis |
| qualification_level |
Master's degree |
| author |
Abd. Samad, Mohd. Yusoff |
| author_facet |
Abd. Samad, Mohd. Yusoff |
| author_sort |
Abd. Samad, Mohd. Yusoff |
| title |
Characterization of Lipase Produced by Thermophilic Fungi Isolated From Pome
|
| title_short |
Characterization of Lipase Produced by Thermophilic Fungi Isolated From Pome
|
| title_full |
Characterization of Lipase Produced by Thermophilic Fungi Isolated From Pome
|
| title_fullStr |
Characterization of Lipase Produced by Thermophilic Fungi Isolated From Pome
|
| title_full_unstemmed |
Characterization of Lipase Produced by Thermophilic Fungi Isolated From Pome
|
| title_sort |
characterization of lipase produced by thermophilic fungi isolated from pome |
| granting_institution |
Universiti Pertanian Malaysia |
| granting_department |
Faculty of Environmental Studies |
| publishDate |
1990 |
| url |
http://psasir.upm.edu.my/id/eprint/8561/1/FSAS_1990_4_A.pdf |
| _version_ |
1747810829599768576 |