Chemical Modification of Lipase and its Immobilization on Polymer Beads for Use in Organic Synthesis

A simple and effective method to produce a more active, stable and practical lipase preparation was identified. Soluble lipase from Candida rugosa was modified with different types of hydrophobic chemical modifying reagents. The esterification activities of the modified lipases were enhanced fo...

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主要作者: Basri, Mahiran
格式: Thesis
语言:English
English
出版: 1993
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spelling my-upm-ir.85812012-08-13T04:38:21Z Chemical Modification of Lipase and its Immobilization on Polymer Beads for Use in Organic Synthesis 1993 Basri, Mahiran A simple and effective method to produce a more active, stable and practical lipase preparation was identified. Soluble lipase from Candida rugosa was modified with different types of hydrophobic chemical modifying reagents. The esterification activities of the modified lipases were enhanced following their modification. The degree of activity enhancement depends on the type and molecular weight of the modifiers used and the degree of modification of the enzyme.A lower degree of enzyme derivatization was required for modification with the high molecular weight modifiers to attain maximal activities. In the case of monomethoxypolyethyleneglycol (PEG), however, maximal activity was attained only after exhaustive modification. The opt imum esterificat ion temperature and preference of fatty acids as acyldonors of the modified lipases were very similar to those of the native enzyme. Both were more active in non-polarsolvents than in polarsolvents. The modified lipases showed higher thermostability, solvent stability and storage stability compared to the native lipase. The lipase modified with PEG 1900 was the most thermostable, and that modified with methyl 4-phenylbutyrimidate (imidoester VI) was the most stable when incubated in benzene for ten days. The bests to rage condition was at low temperature and in the lyophilized form. Proteins - Chemical modification. Organic compounds - Synthesis. 1993 Thesis http://psasir.upm.edu.my/id/eprint/8581/ http://psasir.upm.edu.my/id/eprint/8581/1/FSAS_1993_2_A.pdf application/pdf en public phd doctoral Universiti Pertanian Malaysia Proteins - Chemical modification. Organic compounds - Synthesis. Faculty of Environmental Studies English
institution Universiti Putra Malaysia
collection PSAS Institutional Repository
language English
English
topic Proteins - Chemical modification.
Organic compounds - Synthesis.

spellingShingle Proteins - Chemical modification.
Organic compounds - Synthesis.

Basri, Mahiran
Chemical Modification of Lipase and its Immobilization on Polymer Beads for Use in Organic Synthesis
description A simple and effective method to produce a more active, stable and practical lipase preparation was identified. Soluble lipase from Candida rugosa was modified with different types of hydrophobic chemical modifying reagents. The esterification activities of the modified lipases were enhanced following their modification. The degree of activity enhancement depends on the type and molecular weight of the modifiers used and the degree of modification of the enzyme.A lower degree of enzyme derivatization was required for modification with the high molecular weight modifiers to attain maximal activities. In the case of monomethoxypolyethyleneglycol (PEG), however, maximal activity was attained only after exhaustive modification. The opt imum esterificat ion temperature and preference of fatty acids as acyldonors of the modified lipases were very similar to those of the native enzyme. Both were more active in non-polarsolvents than in polarsolvents. The modified lipases showed higher thermostability, solvent stability and storage stability compared to the native lipase. The lipase modified with PEG 1900 was the most thermostable, and that modified with methyl 4-phenylbutyrimidate (imidoester VI) was the most stable when incubated in benzene for ten days. The bests to rage condition was at low temperature and in the lyophilized form.
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Basri, Mahiran
author_facet Basri, Mahiran
author_sort Basri, Mahiran
title Chemical Modification of Lipase and its Immobilization on Polymer Beads for Use in Organic Synthesis
title_short Chemical Modification of Lipase and its Immobilization on Polymer Beads for Use in Organic Synthesis
title_full Chemical Modification of Lipase and its Immobilization on Polymer Beads for Use in Organic Synthesis
title_fullStr Chemical Modification of Lipase and its Immobilization on Polymer Beads for Use in Organic Synthesis
title_full_unstemmed Chemical Modification of Lipase and its Immobilization on Polymer Beads for Use in Organic Synthesis
title_sort chemical modification of lipase and its immobilization on polymer beads for use in organic synthesis
granting_institution Universiti Pertanian Malaysia
granting_department Faculty of Environmental Studies
publishDate 1993
url http://psasir.upm.edu.my/id/eprint/8581/1/FSAS_1993_2_A.pdf
_version_ 1747810831359279104