Chemical Modification of Lipase and its Immobilization on Polymer Beads for Use in Organic Synthesis

A simple and effective method to produce a more active, stable and practical lipase preparation was identified. Soluble lipase from Candida rugosa was modified with different types of hydrophobic chemical modifying reagents. The esterification activities of the modified lipases were enhanced fo...

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Main Author:	Basri, Mahiran
Format:	Thesis
Language:	English English
Published:	1993
Subjects:	Proteins - Chemical modification. Organic compounds - Synthesis.
Online Access:	http://psasir.upm.edu.my/id/eprint/8581/1/FSAS_1993_2_A.pdf
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id	my-upm-ir.8581
record_format	uketd_dc
spelling	my-upm-ir.85812012-08-13T04:38:21Z Chemical Modification of Lipase and its Immobilization on Polymer Beads for Use in Organic Synthesis 1993 Basri, Mahiran A simple and effective method to produce a more active, stable and practical lipase preparation was identified. Soluble lipase from Candida rugosa was modified with different types of hydrophobic chemical modifying reagents. The esterification activities of the modified lipases were enhanced following their modification. The degree of activity enhancement depends on the type and molecular weight of the modifiers used and the degree of modification of the enzyme.A lower degree of enzyme derivatization was required for modification with the high molecular weight modifiers to attain maximal activities. In the case of monomethoxypolyethyleneglycol (PEG), however, maximal activity was attained only after exhaustive modification. The opt imum esterificat ion temperature and preference of fatty acids as acyldonors of the modified lipases were very similar to those of the native enzyme. Both were more active in non-polarsolvents than in polarsolvents. The modified lipases showed higher thermostability, solvent stability and storage stability compared to the native lipase. The lipase modified with PEG 1900 was the most thermostable, and that modified with methyl 4-phenylbutyrimidate (imidoester VI) was the most stable when incubated in benzene for ten days. The bests to rage condition was at low temperature and in the lyophilized form. Proteins - Chemical modification. Organic compounds - Synthesis. 1993 Thesis http://psasir.upm.edu.my/id/eprint/8581/ http://psasir.upm.edu.my/id/eprint/8581/1/FSAS_1993_2_A.pdf application/pdf en public phd doctoral Universiti Pertanian Malaysia Proteins - Chemical modification. Organic compounds - Synthesis. Faculty of Environmental Studies English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Proteins - Chemical modification. Organic compounds - Synthesis.
spellingShingle	Proteins - Chemical modification. Organic compounds - Synthesis. Basri, Mahiran Chemical Modification of Lipase and its Immobilization on Polymer Beads for Use in Organic Synthesis
description	A simple and effective method to produce a more active, stable and practical lipase preparation was identified. Soluble lipase from Candida rugosa was modified with different types of hydrophobic chemical modifying reagents. The esterification activities of the modified lipases were enhanced following their modification. The degree of activity enhancement depends on the type and molecular weight of the modifiers used and the degree of modification of the enzyme.A lower degree of enzyme derivatization was required for modification with the high molecular weight modifiers to attain maximal activities. In the case of monomethoxypolyethyleneglycol (PEG), however, maximal activity was attained only after exhaustive modification. The opt imum esterificat ion temperature and preference of fatty acids as acyldonors of the modified lipases were very similar to those of the native enzyme. Both were more active in non-polarsolvents than in polarsolvents. The modified lipases showed higher thermostability, solvent stability and storage stability compared to the native lipase. The lipase modified with PEG 1900 was the most thermostable, and that modified with methyl 4-phenylbutyrimidate (imidoester VI) was the most stable when incubated in benzene for ten days. The bests to rage condition was at low temperature and in the lyophilized form.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Basri, Mahiran
author_facet	Basri, Mahiran
author_sort	Basri, Mahiran
title	Chemical Modification of Lipase and its Immobilization on Polymer Beads for Use in Organic Synthesis
title_short	Chemical Modification of Lipase and its Immobilization on Polymer Beads for Use in Organic Synthesis
title_full	Chemical Modification of Lipase and its Immobilization on Polymer Beads for Use in Organic Synthesis
title_fullStr	Chemical Modification of Lipase and its Immobilization on Polymer Beads for Use in Organic Synthesis
title_full_unstemmed	Chemical Modification of Lipase and its Immobilization on Polymer Beads for Use in Organic Synthesis
title_sort	chemical modification of lipase and its immobilization on polymer beads for use in organic synthesis
granting_institution	Universiti Pertanian Malaysia
granting_department	Faculty of Environmental Studies
publishDate	1993
url	http://psasir.upm.edu.my/id/eprint/8581/1/FSAS_1993_2_A.pdf
_version_	1747810831359279104

Chemical Modification of Lipase and its Immobilization on Polymer Beads for Use in Organic Synthesis

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