Production and Characterisation of Thermostable Protease from Bacillus Stearothermophilus Strain F1

Screening and isolation of thermophilic proteolytic bacteria were carried out from composting areas, high temperature ponding systems and areas surrounding palm oil mills. Twelve isolates were positive on Skim Milk A gar (10%) of which 11 produced protease in culture broth. A thermophilic and hi...

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Main Author:	Raja Abd. Rahman, Raja Noor Zaliha
Format:	Thesis
Language:	English English
Published:	1994
Subjects:	Thermophilic bacteria Proteolytic enzymes
Online Access:	http://psasir.upm.edu.my/id/eprint/8589/1/FSAS_1994_7_A.pdf
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id	my-upm-ir.8589
record_format	uketd_dc
spelling	my-upm-ir.85892012-05-07T08:26:40Z Production and Characterisation of Thermostable Protease from Bacillus Stearothermophilus Strain F1 1994 Raja Abd. Rahman, Raja Noor Zaliha Screening and isolation of thermophilic proteolytic bacteria were carried out from composting areas, high temperature ponding systems and areas surrounding palm oil mills. Twelve isolates were positive on Skim Milk A gar (10%) of which 11 produced protease in culture broth. A thermophilic and highly proteolytic isolate identifi edas Bacillus stearothermophilus strain F1 isolated from decomposed oil palm branch, was selected for further study. B. stearothermophilus strain F1 could grow up to 80°C within a broad pH ranges (pH 5 to 11) with an optimal growth temperature and pH at 70°C and 9.5, respectively. The doubling time of this bacteria at 60°C was 70 min. Maximum protease production was achieved after 24 h cultivation when grown in 50 ml medium (pH 10.0) under shaking condition at 60°C. Static condition inhibited protease production but not growth. Cultures grown on peptone (iv) generated the highest amount of protease and lower production was observed when glucose or other rapidly metabolized carbon sources were added. Ammonium salt (0.5%) and amino acids (0.5%) interfered with protease formation whenever they were added to the medium. Although the protease production was calcium independ ent, the presence of the ion at 4.5 mM enhanced the yield by two-fold. Protease production occurred during the midexponential growth phase of the bacterial growth and its onset coincided with sporulation. Thermophilic bacteria Proteolytic enzymes 1994 Thesis http://psasir.upm.edu.my/id/eprint/8589/ http://psasir.upm.edu.my/id/eprint/8589/1/FSAS_1994_7_A.pdf application/pdf en public masters Universiti Pertanian Malaysia Thermophilic bacteria Proteolytic enzymes Faculty of Environmental Studies English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Thermophilic bacteria Proteolytic enzymes
spellingShingle	Thermophilic bacteria Proteolytic enzymes Raja Abd. Rahman, Raja Noor Zaliha Production and Characterisation of Thermostable Protease from Bacillus Stearothermophilus Strain F1
description	Screening and isolation of thermophilic proteolytic bacteria were carried out from composting areas, high temperature ponding systems and areas surrounding palm oil mills. Twelve isolates were positive on Skim Milk A gar (10%) of which 11 produced protease in culture broth. A thermophilic and highly proteolytic isolate identifi edas Bacillus stearothermophilus strain F1 isolated from decomposed oil palm branch, was selected for further study. B. stearothermophilus strain F1 could grow up to 80°C within a broad pH ranges (pH 5 to 11) with an optimal growth temperature and pH at 70°C and 9.5, respectively. The doubling time of this bacteria at 60°C was 70 min. Maximum protease production was achieved after 24 h cultivation when grown in 50 ml medium (pH 10.0) under shaking condition at 60°C. Static condition inhibited protease production but not growth. Cultures grown on peptone (iv) generated the highest amount of protease and lower production was observed when glucose or other rapidly metabolized carbon sources were added. Ammonium salt (0.5%) and amino acids (0.5%) interfered with protease formation whenever they were added to the medium. Although the protease production was calcium independ ent, the presence of the ion at 4.5 mM enhanced the yield by two-fold. Protease production occurred during the midexponential growth phase of the bacterial growth and its onset coincided with sporulation.
format	Thesis
qualification_level	Master's degree
author	Raja Abd. Rahman, Raja Noor Zaliha
author_facet	Raja Abd. Rahman, Raja Noor Zaliha
author_sort	Raja Abd. Rahman, Raja Noor Zaliha
title	Production and Characterisation of Thermostable Protease from Bacillus Stearothermophilus Strain F1
title_short	Production and Characterisation of Thermostable Protease from Bacillus Stearothermophilus Strain F1
title_full	Production and Characterisation of Thermostable Protease from Bacillus Stearothermophilus Strain F1
title_fullStr	Production and Characterisation of Thermostable Protease from Bacillus Stearothermophilus Strain F1
title_full_unstemmed	Production and Characterisation of Thermostable Protease from Bacillus Stearothermophilus Strain F1
title_sort	production and characterisation of thermostable protease from bacillus stearothermophilus strain f1
granting_institution	Universiti Pertanian Malaysia
granting_department	Faculty of Environmental Studies
publishDate	1994
url	http://psasir.upm.edu.my/id/eprint/8589/1/FSAS_1994_7_A.pdf
_version_	1747810833307533312

Production and Characterisation of Thermostable Protease from Bacillus Stearothermophilus Strain F1

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