Antioxidative activities and isolation of phenolic compounds from the root, fruit and leaf of Mengkudu (Morinda citrifolia L.)
This study was conducted with the objective of evaluating the antioxidative activities of extracts from different parts of Mengkudu (Morinda citrifolia L.) including leaf, fruit and root Methanol, petroleum ether, water and ethyl acetate were used as extraction solvent. The anti oxidative activit...
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2003
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| Online Access: | http://psasir.upm.edu.my/id/eprint/8732/1/FSMB_2003_25%20IR.pdf |
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Universiti Putra Malaysia |
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| language |
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| advisor |
Abdul Hamid, Azizah |
| topic |
Antioxidants Phenols Plant Extracts |
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Antioxidants Phenols Plant Extracts Mohd Zin, Zamzahaila Antioxidative activities and isolation of phenolic compounds from the root, fruit and leaf of Mengkudu (Morinda citrifolia L.) |
| description |
This study was conducted with the objective of evaluating the antioxidative activities of
extracts from different parts of Mengkudu (Morinda citrifolia L.) including leaf, fruit
and root Methanol, petroleum ether, water and ethyl acetate were used as extraction
solvent. The anti oxidative activities assays were done using ferric thiocyanate method
(FTC) and thiobarbituric acid test (TBA). Furthermore, the activities of these extracts
were then compared to that of a-tocopherol and butylated hydroxyl tolune (BHT). Effect
of solvent types and concentration of extract used on antioxidative activities of M.
citrifolia were also carried out. Meanwhile, isolation of phenolic compounds was carried
out using Sephadex LH-20 column chromatography. Identification and quantification of
the common flavonoids in the separated fractions were carried out using a reversed
phase high-performance liquid chromatography (RP-HPLC) procedures on a Symmetry
CIS colwnn with water-methanol gradient elution system. Results showed that methanol
root extract exhibited higher activity compared to that of fruit and leaf extract of the same with no significant (p<O.05) difference as compared to that of a-tocopherol and
BHT. The result also showed that methanol gave highest yield of extract compared to
that of water, petroleum ether and ethyl acetate extraction. Meanwhile, both polar and
non-polar extract of M citrifolia L. exhibited appreciable activity. Increasing
concentration of methanol root extracts from 1000 to 5000 ppm do not significantly
increased the anti oxidative activity. On the other hand, there was significant (p<0.05)
difference in antioxidative activities of methanol leaf and fruit extracts when
concentrations were increased. Similarly, increasing concentrations of water extracts
from 1000 to 5000 ppm do not significantly increased the antioxidative activity of root
and leaf of M cirrifolia L. However, there was significant (P<0.05) difference in
anti oxidative activities of fruit extract at 1000 ppm compared to 2000 ppm. At 3000 ppm
onwards there were no significant (p<0.05) difference in activities. Result also showed
that at 3000 ppm, water extract of root, fruit and leaf of M citrifolia L. exhibited no
significant (p<0.05) difference in activities dum that of either a -tocopherol or BHT.
Meanwhile, increasing concentration of petroleum ether extract significantly increased
the anti oxidative activity of all parts of M citrifolia. The result also revealed that the
antioxidative activities exhibited by petroleum ether root extract was significantly
(p<0.05) higher than that of either a -tocopherol or BHT at concentration more than
4000 ppm. Similar observation was found in fruit and leaf extract of same solvent at
5000 ppm, where fruit and leaf extract exhibited significantly (p<0.05) higher activity
than that of either a -tocopherol or BHT. Increasing concentration of ethyl acetate leaf
extract from 1000 ppm to 5000 ppm do not significantly increased the antioxidative
activities. On the other hand, at 3000 ppm onwards there was significant (p<0.05)difference in anti oxidative activities of root and fruit extracts when concentrations were
increased. Result also showed that at 3000 ppm and 4000 ppm, there was no significant
(P<0.05) difference in the activities exhibited by root and leaf extract of same solvent
than that of either (l -tocopherol or BHT. Meanwhile ethyl acetate fruit extract exhibited
significantly higher activity than that of either (l-tocopherol or BHT at the same
concentration tested. From this study it can be seen that different parts of M citrifolia
exhibited varying degrees of antioxidative activities when different solvents were used
in extraction procedures. Of the four solvents used, ethyl acetate is the best solvent for
extracting antioxidative compounds from different parts (root, fruit and leaf) of Morinda
citrifolia L. compared to other solvent. Isolation of phenolic compounds mainly focused
on the separation and identification of antioxidants from methanol extract because of the
polar properties of phenolic compounds especially flavonoids, which are known to be
potent antioxidant. All fractions were seen to contain different amount of total phenolic
compound and exhibited considerably high antioxidative activity. Identification of
flavonoid successfully determines the individual active compound present in M
citrifolia. Major component determine including catechin and epicatechin, which are
known to be potent antioxidant. |
| format |
Thesis |
| qualification_level |
Master's degree |
| author |
Mohd Zin, Zamzahaila |
| author_facet |
Mohd Zin, Zamzahaila |
| author_sort |
Mohd Zin, Zamzahaila |
| title |
Antioxidative activities and isolation of phenolic compounds from the root, fruit and leaf of Mengkudu (Morinda citrifolia L.) |
| title_short |
Antioxidative activities and isolation of phenolic compounds from the root, fruit and leaf of Mengkudu (Morinda citrifolia L.) |
| title_full |
Antioxidative activities and isolation of phenolic compounds from the root, fruit and leaf of Mengkudu (Morinda citrifolia L.) |
| title_fullStr |
Antioxidative activities and isolation of phenolic compounds from the root, fruit and leaf of Mengkudu (Morinda citrifolia L.) |
| title_full_unstemmed |
Antioxidative activities and isolation of phenolic compounds from the root, fruit and leaf of Mengkudu (Morinda citrifolia L.) |
| title_sort |
antioxidative activities and isolation of phenolic compounds from the root, fruit and leaf of mengkudu (morinda citrifolia l.) |
| granting_institution |
Universiti Putra Malaysia |
| granting_department |
Food Science and Technology |
| publishDate |
2003 |
| url |
http://psasir.upm.edu.my/id/eprint/8732/1/FSMB_2003_25%20IR.pdf |
| _version_ |
1794018778781057024 |
| spelling |
my-upm-ir.87322024-01-23T07:52:00Z Antioxidative activities and isolation of phenolic compounds from the root, fruit and leaf of Mengkudu (Morinda citrifolia L.) 2003-10 Mohd Zin, Zamzahaila This study was conducted with the objective of evaluating the antioxidative activities of extracts from different parts of Mengkudu (Morinda citrifolia L.) including leaf, fruit and root Methanol, petroleum ether, water and ethyl acetate were used as extraction solvent. The anti oxidative activities assays were done using ferric thiocyanate method (FTC) and thiobarbituric acid test (TBA). Furthermore, the activities of these extracts were then compared to that of a-tocopherol and butylated hydroxyl tolune (BHT). Effect of solvent types and concentration of extract used on antioxidative activities of M. citrifolia were also carried out. Meanwhile, isolation of phenolic compounds was carried out using Sephadex LH-20 column chromatography. Identification and quantification of the common flavonoids in the separated fractions were carried out using a reversed phase high-performance liquid chromatography (RP-HPLC) procedures on a Symmetry CIS colwnn with water-methanol gradient elution system. Results showed that methanol root extract exhibited higher activity compared to that of fruit and leaf extract of the same with no significant (p<O.05) difference as compared to that of a-tocopherol and BHT. The result also showed that methanol gave highest yield of extract compared to that of water, petroleum ether and ethyl acetate extraction. Meanwhile, both polar and non-polar extract of M citrifolia L. exhibited appreciable activity. Increasing concentration of methanol root extracts from 1000 to 5000 ppm do not significantly increased the anti oxidative activity. On the other hand, there was significant (p<0.05) difference in antioxidative activities of methanol leaf and fruit extracts when concentrations were increased. Similarly, increasing concentrations of water extracts from 1000 to 5000 ppm do not significantly increased the antioxidative activity of root and leaf of M cirrifolia L. However, there was significant (P<0.05) difference in anti oxidative activities of fruit extract at 1000 ppm compared to 2000 ppm. At 3000 ppm onwards there were no significant (p<0.05) difference in activities. Result also showed that at 3000 ppm, water extract of root, fruit and leaf of M citrifolia L. exhibited no significant (p<0.05) difference in activities dum that of either a -tocopherol or BHT. Meanwhile, increasing concentration of petroleum ether extract significantly increased the anti oxidative activity of all parts of M citrifolia. The result also revealed that the antioxidative activities exhibited by petroleum ether root extract was significantly (p<0.05) higher than that of either a -tocopherol or BHT at concentration more than 4000 ppm. Similar observation was found in fruit and leaf extract of same solvent at 5000 ppm, where fruit and leaf extract exhibited significantly (p<0.05) higher activity than that of either a -tocopherol or BHT. Increasing concentration of ethyl acetate leaf extract from 1000 ppm to 5000 ppm do not significantly increased the antioxidative activities. On the other hand, at 3000 ppm onwards there was significant (p<0.05)difference in anti oxidative activities of root and fruit extracts when concentrations were increased. Result also showed that at 3000 ppm and 4000 ppm, there was no significant (P<0.05) difference in the activities exhibited by root and leaf extract of same solvent than that of either (l -tocopherol or BHT. Meanwhile ethyl acetate fruit extract exhibited significantly higher activity than that of either (l-tocopherol or BHT at the same concentration tested. From this study it can be seen that different parts of M citrifolia exhibited varying degrees of antioxidative activities when different solvents were used in extraction procedures. Of the four solvents used, ethyl acetate is the best solvent for extracting antioxidative compounds from different parts (root, fruit and leaf) of Morinda citrifolia L. compared to other solvent. Isolation of phenolic compounds mainly focused on the separation and identification of antioxidants from methanol extract because of the polar properties of phenolic compounds especially flavonoids, which are known to be potent antioxidant. All fractions were seen to contain different amount of total phenolic compound and exhibited considerably high antioxidative activity. Identification of flavonoid successfully determines the individual active compound present in M citrifolia. Major component determine including catechin and epicatechin, which are known to be potent antioxidant. Antioxidants Phenols Plant Extracts 2003-10 Thesis http://psasir.upm.edu.my/id/eprint/8732/ http://psasir.upm.edu.my/id/eprint/8732/1/FSMB_2003_25%20IR.pdf text en public masters Universiti Putra Malaysia Antioxidants Phenols Plant Extracts Food Science and Technology Abdul Hamid, Azizah English |