Antimigraine activity of Ficus deltoidea jack aqueous extract in mice and its possible mechanisms

Migraine is a disabling headache disorder characterized by throbbing headache and associated with various symptoms namely nausea, vomiting and heightened sensitivity to touch and smell. Ficus deltoidea is an herbaceous plant used traditionally in treating pain and headache. As phytomedicine produ...

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Main Author:	Salihan, Safuraa
Format:	Thesis
Language:	English
Published:	2020
Subjects:	Migraine Disorders - therapy
Online Access:	http://psasir.upm.edu.my/id/eprint/90464/1/FPSK%28p%29%202020%203%20-%20IR.pdf
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spelling	my-upm-ir.904642021-08-02T02:20:54Z Antimigraine activity of Ficus deltoidea jack aqueous extract in mice and its possible mechanisms 2020-01 Salihan, Safuraa Migraine is a disabling headache disorder characterized by throbbing headache and associated with various symptoms namely nausea, vomiting and heightened sensitivity to touch and smell. Ficus deltoidea is an herbaceous plant used traditionally in treating pain and headache. As phytomedicine produce alternative therapeutic strategies for migraine pain, the aim of this study is to evaluate the antimigraine properties of Ficus deltoidea (var Trengganuensis) aqueous extract (FDA) and its possible mechanisms. In nonspecific antimigraine study, using animal model of nociception, administration of FDA produced significant antinociceptive effect in acetic acid-induced abdominal writhing test, early and late phase of formalin test and in the hot plate test. In specific antimigraine study, nitroglycerin (NTG)-induced migraine model was used. It is the most studied and well accepted model in antimigraine drug testing. Preliminary tests were done verifying and optimizing the use of this model. The effect of FDA was tested in NTG-induced hyperalgesia using formalin and hot plate test. It was found that FDA produced significant inhibition in both early and late phase of formalin test and significant increase in respond latency in hot plate test. In addition, treatment with FDA significantly reduced the NTG-induced c-fos expression in trigeminal nucleus caudalis (TNC), a relay center in migraine. This study also explored the mechanism of FDA through peripheral and central sensitization, and involvement of serotonergic and dopaminergic pathways. The involvement of FDA in peripheral sensitization was done using kainic acid-induced hyperalgesia in hot plate test, showing significant increase in response latency in group receiving FDA compared to control. In study of central sensitization, using NTG-induced mechanical allodynia in von Frey test, FDA group produced significant improvement in paw withdrawal threshold compared to control. Studies on serotonergic and dopaminergic systems involvement was done by studying the effect of FDA on 5-hydroxytryptophan(l-5-HTP)-induced serotonin syndrome and apomorphine-induced climbing behavior. Results showed FDA significantly inhibited l-5-HTP-induced serotonin syndrome and apomorphine-induced climbing activity. In addition, FDA significantly inhibited NTG induced plasma CGRP using ELISA. These findings suggested that FDA possessed antimigraine activity through inhibition of peripheral and central sensitization with possibility of involvement of dopaminergic and serotonergic mechanism and CGRP inhibition. Migraine Disorders - therapy 2020-01 Thesis http://psasir.upm.edu.my/id/eprint/90464/ http://psasir.upm.edu.my/id/eprint/90464/1/FPSK%28p%29%202020%203%20-%20IR.pdf text en public doctoral Universiti Putra Malaysia Migraine Disorders - therapy Mohd Moklas, Mohamad Aris
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
advisor	Mohd Moklas, Mohamad Aris
topic	Migraine Disorders - therapy
spellingShingle	Migraine Disorders - therapy Salihan, Safuraa Antimigraine activity of Ficus deltoidea jack aqueous extract in mice and its possible mechanisms
description	Migraine is a disabling headache disorder characterized by throbbing headache and associated with various symptoms namely nausea, vomiting and heightened sensitivity to touch and smell. Ficus deltoidea is an herbaceous plant used traditionally in treating pain and headache. As phytomedicine produce alternative therapeutic strategies for migraine pain, the aim of this study is to evaluate the antimigraine properties of Ficus deltoidea (var Trengganuensis) aqueous extract (FDA) and its possible mechanisms. In nonspecific antimigraine study, using animal model of nociception, administration of FDA produced significant antinociceptive effect in acetic acid-induced abdominal writhing test, early and late phase of formalin test and in the hot plate test. In specific antimigraine study, nitroglycerin (NTG)-induced migraine model was used. It is the most studied and well accepted model in antimigraine drug testing. Preliminary tests were done verifying and optimizing the use of this model. The effect of FDA was tested in NTG-induced hyperalgesia using formalin and hot plate test. It was found that FDA produced significant inhibition in both early and late phase of formalin test and significant increase in respond latency in hot plate test. In addition, treatment with FDA significantly reduced the NTG-induced c-fos expression in trigeminal nucleus caudalis (TNC), a relay center in migraine. This study also explored the mechanism of FDA through peripheral and central sensitization, and involvement of serotonergic and dopaminergic pathways. The involvement of FDA in peripheral sensitization was done using kainic acid-induced hyperalgesia in hot plate test, showing significant increase in response latency in group receiving FDA compared to control. In study of central sensitization, using NTG-induced mechanical allodynia in von Frey test, FDA group produced significant improvement in paw withdrawal threshold compared to control. Studies on serotonergic and dopaminergic systems involvement was done by studying the effect of FDA on 5-hydroxytryptophan(l-5-HTP)-induced serotonin syndrome and apomorphine-induced climbing behavior. Results showed FDA significantly inhibited l-5-HTP-induced serotonin syndrome and apomorphine-induced climbing activity. In addition, FDA significantly inhibited NTG induced plasma CGRP using ELISA. These findings suggested that FDA possessed antimigraine activity through inhibition of peripheral and central sensitization with possibility of involvement of dopaminergic and serotonergic mechanism and CGRP inhibition.
format	Thesis
qualification_level	Doctorate
author	Salihan, Safuraa
author_facet	Salihan, Safuraa
author_sort	Salihan, Safuraa
title	Antimigraine activity of Ficus deltoidea jack aqueous extract in mice and its possible mechanisms
title_short	Antimigraine activity of Ficus deltoidea jack aqueous extract in mice and its possible mechanisms
title_full	Antimigraine activity of Ficus deltoidea jack aqueous extract in mice and its possible mechanisms
title_fullStr	Antimigraine activity of Ficus deltoidea jack aqueous extract in mice and its possible mechanisms
title_full_unstemmed	Antimigraine activity of Ficus deltoidea jack aqueous extract in mice and its possible mechanisms
title_sort	antimigraine activity of ficus deltoidea jack aqueous extract in mice and its possible mechanisms
granting_institution	Universiti Putra Malaysia
publishDate	2020
url	http://psasir.upm.edu.my/id/eprint/90464/1/FPSK%28p%29%202020%203%20-%20IR.pdf
_version_	1747813626179223552

Antimigraine activity of Ficus deltoidea jack aqueous extract in mice and its possible mechanisms

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