Electrochemical enhancement using iron oxide-gold nanocomposite for detection of tuberculosis based on rGO-APTES modified screen printed electrode
A new electrochemical immunosensor based on Fe3O4-Au nanocomposite (NC) as signal enhancement was proposed for the detection of Mycobacterium Tuberculosis ESAT-6-like protein EsxB. In this study, Fe3O4-Au NC was synthesis via coprecipitation method and size of NC was viewed under high resolution...
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| المؤلف الرئيسي: | |
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| التنسيق: | أطروحة |
| اللغة: | English |
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2018
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| الوصول للمادة أونلاين: | http://psasir.upm.edu.my/id/eprint/90513/1/FS%202019%2079%20-%20IR.pdf |
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my-upm-ir.905132021-08-11T00:58:57Z Electrochemical enhancement using iron oxide-gold nanocomposite for detection of tuberculosis based on rGO-APTES modified screen printed electrode 2018-04 Syed Amri, Syazana Ameera A new electrochemical immunosensor based on Fe3O4-Au nanocomposite (NC) as signal enhancement was proposed for the detection of Mycobacterium Tuberculosis ESAT-6-like protein EsxB. In this study, Fe3O4-Au NC was synthesis via coprecipitation method and size of NC was viewed under high resolution transmission electron microscopy (HRTEM) with average diameter of 1.629-1.915 nm. Meanwhile, X-ray diffraction (XRD) analysis confirmed that Fe3O4-Au NC exhibited all possible peaks of Fe3O4 and new peaks at 38.37° (111) and 44.63° (200) are belongs to crystal plane of metallic gold. Additionally, UV-Vis absorption study shows peak at 545 nm was appeared indicating that Au shell was successfully coated on Fe3O4 NC. Characterization by FTIR demonstrated that the peak at 1670 cm-1 assigned to C=O group and peak at 3280 cm-1 indicated O-H group. The peak at 710 cm-1 represented CS stretching. The IR spectra confirms successful functionalization of the Fe3O4-Au NC with ME-MDDA linker. In this study, rGO-APTES have been used to modify screen printed electrode (SPE) to serve as immobilization matrix for immunoassay interaction. The result from RAMAN spectroscopy, Field Emission Scanning Electron Microscope (FE-SEM) and Energy Dispersive X-ray Spectroscopy (EDX) confirmed rGO-APTES modified SPE successfully reduced. Additionally, the morphology of rGO-APTES modified SPE also revealed smoother surface and ready for immobilization of capture antibody (Ab). The effective surface area value of rGO-APTES modified SPE also has increase about 20 times larger compared to bare SPE of based on cyclic voltammetry (CV) studies. Moreover, the ratio of Ipc/Ipa = 0.9867 showed electron transfer process is towards reversible kinetics. The rGO-APTES modified SPE was further explored as immunoassays matrix for Ab1 tagged with Fe3O4-Au NC (Ab1- Fe3O4-Au) and further interact with Mycobacterium Tuberculosis ESAT-6-like protein EsxB were monitored by differential pulse voltammetry (DPV) technique using 0.1 M PBS as electrolyte. As a result, the decreased of peak current of Ab from 3.996 μA to 3.863 μA occurs after immobilization on rGO-APTES modified SPE confirmed that electron transfer resistance increased. At optimal condition, the DPV was studied in different concentration of Mycobacterium Tuberculosis ESAT-6-like protein EsxB. The DPV current of immunosensor electrode increased as the Mycobacterium Tuberculosis ESAT-6-like protein EsxB increased. In this study, the lowest TB concentration that could be determined statistical by resulting immunosensor was calculated as 1.32 ng/mL. The Relative Standard Deviation (RSD) of the measurements for five electrodes was 6.4% suggesting the precision and reproducibility of the immunosensor was quite good. Chemical engineering Electrochemical sensors Electrodes 2018-04 Thesis http://psasir.upm.edu.my/id/eprint/90513/ http://psasir.upm.edu.my/id/eprint/90513/1/FS%202019%2079%20-%20IR.pdf text en public masters Universiti Putra Malaysia Chemical engineering Electrochemical sensors Electrodes Yusof, Nor Azah |
| institution |
Universiti Putra Malaysia |
| collection |
PSAS Institutional Repository |
| language |
English |
| advisor |
Yusof, Nor Azah |
| topic |
Chemical engineering Electrochemical sensors Electrodes |
| spellingShingle |
Chemical engineering Electrochemical sensors Electrodes Syed Amri, Syazana Ameera Electrochemical enhancement using iron oxide-gold nanocomposite for detection of tuberculosis based on rGO-APTES modified screen printed electrode |
| description |
A new electrochemical immunosensor based on Fe3O4-Au nanocomposite (NC) as
signal enhancement was proposed for the detection of Mycobacterium Tuberculosis
ESAT-6-like protein EsxB. In this study, Fe3O4-Au NC was synthesis via coprecipitation
method and size of NC was viewed under high resolution transmission
electron microscopy (HRTEM) with average diameter of 1.629-1.915 nm. Meanwhile,
X-ray diffraction (XRD) analysis confirmed that Fe3O4-Au NC exhibited all possible
peaks of Fe3O4 and new peaks at 38.37° (111) and 44.63° (200) are belongs to crystal
plane of metallic gold. Additionally, UV-Vis absorption study shows peak at 545 nm
was appeared indicating that Au shell was successfully coated on Fe3O4 NC.
Characterization by FTIR demonstrated that the peak at 1670 cm-1 assigned to C=O
group and peak at 3280 cm-1 indicated O-H group. The peak at 710 cm-1 represented CS
stretching. The IR spectra confirms successful functionalization of the Fe3O4-Au NC
with ME-MDDA linker. In this study, rGO-APTES have been used to modify screen
printed electrode (SPE) to serve as immobilization matrix for immunoassay interaction.
The result from RAMAN spectroscopy, Field Emission Scanning Electron Microscope
(FE-SEM) and Energy Dispersive X-ray Spectroscopy (EDX) confirmed rGO-APTES
modified SPE successfully reduced. Additionally, the morphology of rGO-APTES
modified SPE also revealed smoother surface and ready for immobilization of capture
antibody (Ab). The effective surface area value of rGO-APTES modified SPE also has
increase about 20 times larger compared to bare SPE of based on cyclic voltammetry
(CV) studies. Moreover, the ratio of Ipc/Ipa = 0.9867 showed electron transfer process
is towards reversible kinetics. The rGO-APTES modified SPE was further explored as
immunoassays matrix for Ab1 tagged with Fe3O4-Au NC (Ab1- Fe3O4-Au) and further
interact with Mycobacterium Tuberculosis ESAT-6-like protein EsxB were monitored
by differential pulse voltammetry (DPV) technique using 0.1 M PBS as electrolyte. As
a result, the decreased of peak current of Ab from 3.996 μA to 3.863 μA occurs after
immobilization on rGO-APTES modified SPE confirmed that electron transfer
resistance increased. At optimal condition, the DPV was studied in different concentration of Mycobacterium Tuberculosis ESAT-6-like protein EsxB. The DPV
current of immunosensor electrode increased as the Mycobacterium Tuberculosis
ESAT-6-like protein EsxB increased. In this study, the lowest TB concentration that
could be determined statistical by resulting immunosensor was calculated as 1.32
ng/mL. The Relative Standard Deviation (RSD) of the measurements for five
electrodes was 6.4% suggesting the precision and reproducibility of the immunosensor
was quite good. |
| format |
Thesis |
| qualification_level |
Master's degree |
| author |
Syed Amri, Syazana Ameera |
| author_facet |
Syed Amri, Syazana Ameera |
| author_sort |
Syed Amri, Syazana Ameera |
| title |
Electrochemical enhancement using iron oxide-gold nanocomposite for detection of tuberculosis based on rGO-APTES modified screen printed electrode |
| title_short |
Electrochemical enhancement using iron oxide-gold nanocomposite for detection of tuberculosis based on rGO-APTES modified screen printed electrode |
| title_full |
Electrochemical enhancement using iron oxide-gold nanocomposite for detection of tuberculosis based on rGO-APTES modified screen printed electrode |
| title_fullStr |
Electrochemical enhancement using iron oxide-gold nanocomposite for detection of tuberculosis based on rGO-APTES modified screen printed electrode |
| title_full_unstemmed |
Electrochemical enhancement using iron oxide-gold nanocomposite for detection of tuberculosis based on rGO-APTES modified screen printed electrode |
| title_sort |
electrochemical enhancement using iron oxide-gold nanocomposite for detection of tuberculosis based on rgo-aptes modified screen printed electrode |
| granting_institution |
Universiti Putra Malaysia |
| publishDate |
2018 |
| url |
http://psasir.upm.edu.my/id/eprint/90513/1/FS%202019%2079%20-%20IR.pdf |
| _version_ |
1747813631834193920 |