Enhancement of hydrogen and methane production from co-digestion of food waste and chicken manure

The demand for clean energy from renewable resources stimulates biohydrogen and biomethane production from agro-food waste as an alternative fuel to replace fossil fuel. A combined production of biohydrogen and biomethane has attracted growing attention of researchers and industries worldwide du...

Full description

Saved in:
Bibliographic Details
Main Author: Mat Saad, Mohd Faiz
Format: Thesis
Language:English
Published: 2017
Subjects:
Online Access:http://psasir.upm.edu.my/id/eprint/90663/1/FBSB%202018%2066%20-%20IR.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-upm-ir.90663
record_format uketd_dc
spelling my-upm-ir.906632021-08-30T00:30:12Z Enhancement of hydrogen and methane production from co-digestion of food waste and chicken manure 2017-03 Mat Saad, Mohd Faiz The demand for clean energy from renewable resources stimulates biohydrogen and biomethane production from agro-food waste as an alternative fuel to replace fossil fuel. A combined production of biohydrogen and biomethane has attracted growing attention of researchers and industries worldwide due to their potential as fuel substitute. Biosynthesis of biohydrogen and biomethane from food waste and chicken manure fermentation initiates clean technologies for energy generation thus provide the solution for waste treatment. Despite that, biogas production of hydrogen and methane have limiting factors that relate to soluble metabolites and active microorganisms. This inhibition effects can be overcome by optimizing several factors for biohydrogen and biomethane production. The objectives of this study were to determine the best ratio of food waste and chicken manure for biogas production in batch fermentation and to evaluate the effect of different inoculums and heat treatment upon selected inoculum on biohydrogen and biomethane production besides the microbial diversity in the fermentation using Next Generation Sequencing (NGS) of 16S ribosomal RNA were also carried out. The batch fermentation was conducted using 150 mL serum bottles incubated in anaerobic condition. Food waste with composition ratios of 3:1:1 of carbohydrates, protein and fiber were used as substrate added with chicken manure freshly collected from poultry farm. Biohydrogen and biomethane production were tested for the effects of different substrate ratio, different inoculums and heat treatment on selected inoculums. Temperature and initial pH were kept constant at 35°C and initial pH 7. Biohydrogen and biomethane from food waste and chicken manure was performed at different ratio (40:60, 50:50, 60:40 and 70:30 (v/v)) inoculated with aeration tank sludge (ATS), return activated sludge (RAS) and palm oil mill effluent (POME) sludge. Heat treatment was carried out at 80°C for 20 minutes to eliminate the nonsporing bacteria. Biogas was collected daily throughout 10 days fermentation and the composition of hydrogen and methane in the biogas was analyzed by gas chromatography. The highest biogas yield obtained was 111.72 NmL/g TSS for the experiment conducted at 50:50 (v/v) substrate ratio added with RAS as inoculum without heat treatment. The highest percentages of hydrogen and methane produced were 53.35% and 52.85%, respectively. Microbial assessment was performed by using Next Generation Sequencing (NGS) of 16S ribosomal RNA technique. Clostridium sp. was related to biohydrogen production methanotroph such as Cyclobacteriaceae, Saprospiraceae and Chloroflexi that were inhibited after the heat treatment. Heat treatment of inoculums is not suitable for the production of both biohydrogen and biomethane since it inhibits the methanogens. Thus, controlling operating conditions were important for hydrogen-producing bacteria as well as methanogens for biohydrogen and biomethane production. Hydrogen Methane Food waste 2017-03 Thesis http://psasir.upm.edu.my/id/eprint/90663/ http://psasir.upm.edu.my/id/eprint/90663/1/FBSB%202018%2066%20-%20IR.pdf text en public masters Universiti Putra Malaysia Hydrogen Methane Food waste Abdul Rahman, Nor'aini
institution Universiti Putra Malaysia
collection PSAS Institutional Repository
language English
advisor Abdul Rahman, Nor'aini
topic Hydrogen
Methane
Food waste
spellingShingle Hydrogen
Methane
Food waste
Mat Saad, Mohd Faiz
Enhancement of hydrogen and methane production from co-digestion of food waste and chicken manure
description The demand for clean energy from renewable resources stimulates biohydrogen and biomethane production from agro-food waste as an alternative fuel to replace fossil fuel. A combined production of biohydrogen and biomethane has attracted growing attention of researchers and industries worldwide due to their potential as fuel substitute. Biosynthesis of biohydrogen and biomethane from food waste and chicken manure fermentation initiates clean technologies for energy generation thus provide the solution for waste treatment. Despite that, biogas production of hydrogen and methane have limiting factors that relate to soluble metabolites and active microorganisms. This inhibition effects can be overcome by optimizing several factors for biohydrogen and biomethane production. The objectives of this study were to determine the best ratio of food waste and chicken manure for biogas production in batch fermentation and to evaluate the effect of different inoculums and heat treatment upon selected inoculum on biohydrogen and biomethane production besides the microbial diversity in the fermentation using Next Generation Sequencing (NGS) of 16S ribosomal RNA were also carried out. The batch fermentation was conducted using 150 mL serum bottles incubated in anaerobic condition. Food waste with composition ratios of 3:1:1 of carbohydrates, protein and fiber were used as substrate added with chicken manure freshly collected from poultry farm. Biohydrogen and biomethane production were tested for the effects of different substrate ratio, different inoculums and heat treatment on selected inoculums. Temperature and initial pH were kept constant at 35°C and initial pH 7. Biohydrogen and biomethane from food waste and chicken manure was performed at different ratio (40:60, 50:50, 60:40 and 70:30 (v/v)) inoculated with aeration tank sludge (ATS), return activated sludge (RAS) and palm oil mill effluent (POME) sludge. Heat treatment was carried out at 80°C for 20 minutes to eliminate the nonsporing bacteria. Biogas was collected daily throughout 10 days fermentation and the composition of hydrogen and methane in the biogas was analyzed by gas chromatography. The highest biogas yield obtained was 111.72 NmL/g TSS for the experiment conducted at 50:50 (v/v) substrate ratio added with RAS as inoculum without heat treatment. The highest percentages of hydrogen and methane produced were 53.35% and 52.85%, respectively. Microbial assessment was performed by using Next Generation Sequencing (NGS) of 16S ribosomal RNA technique. Clostridium sp. was related to biohydrogen production methanotroph such as Cyclobacteriaceae, Saprospiraceae and Chloroflexi that were inhibited after the heat treatment. Heat treatment of inoculums is not suitable for the production of both biohydrogen and biomethane since it inhibits the methanogens. Thus, controlling operating conditions were important for hydrogen-producing bacteria as well as methanogens for biohydrogen and biomethane production.
format Thesis
qualification_level Master's degree
author Mat Saad, Mohd Faiz
author_facet Mat Saad, Mohd Faiz
author_sort Mat Saad, Mohd Faiz
title Enhancement of hydrogen and methane production from co-digestion of food waste and chicken manure
title_short Enhancement of hydrogen and methane production from co-digestion of food waste and chicken manure
title_full Enhancement of hydrogen and methane production from co-digestion of food waste and chicken manure
title_fullStr Enhancement of hydrogen and methane production from co-digestion of food waste and chicken manure
title_full_unstemmed Enhancement of hydrogen and methane production from co-digestion of food waste and chicken manure
title_sort enhancement of hydrogen and methane production from co-digestion of food waste and chicken manure
granting_institution Universiti Putra Malaysia
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/90663/1/FBSB%202018%2066%20-%20IR.pdf
_version_ 1747813644363628544