Cloning and Expression of Vp2 Gene of Chicken Anemia Vurus Strain Cux-1 in Lactococcus Lactis Mg1363

Cloning and Expression of Vp2 Gene of Chicken Anemia Vurus Strain Cux-1 in Lactococcus Lactis Mg1363

In this study, the lactococcal plasmid vector pMG36e, was exploited for the cloning, replication and expression of a viral protein-2 (VP2) gene in the bacterial host, Lactococcus lactis strain MG1363. The VP2 gene (0.65 kb) of chicken anemia virus (CAV) of strain Cuxhaven-l was amplified from vir...

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Main Author: Ahmat, Nurfilza
Format: Thesis
Language:English
English
Published: 2001
Subjects:
Lactococcus - Genetics
Molecular cloning
Online Access:http://psasir.upm.edu.my/id/eprint/9468/1/FSAS_2001_41.pdf
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spelling my-upm-ir.94682024-03-06T01:52:41Z Cloning and Expression of Vp2 Gene of Chicken Anemia Vurus Strain Cux-1 in Lactococcus Lactis Mg1363 2001-03 Ahmat, Nurfilza In this study, the lactococcal plasmid vector pMG36e, was exploited for the cloning, replication and expression of a viral protein-2 (VP2) gene in the bacterial host, Lactococcus lactis strain MG1363. The VP2 gene (0.65 kb) of chicken anemia virus (CAV) of strain Cuxhaven-l was amplified from viral genomic DNA by polymerase chain reaction (PCR) using the VP2FX and VP2RSai primers (Table 6), cloned into the expression vector pMG36e, and electrotransformed into the L. lactis MG1363 host. Sequencing of the recombinant plasmid pMG36e-VP2 using the Forward and Reverse primers (Table 6), showed 99% homology of the VP2 insert with that of the published VP2 CAY Cux-l sequence. SDS-PAGE and Western blot hybridization of L. lactis (pMG36e-VP2; 4.3 kb), showed no significant protein band of VP2 gene product (24 kDa). However, the gene was transcribed under the P32 promoter of the expression vector pMG36e as shown by a 0.7 kb reverse-transcribed product of the VP2 gene. Lactococcus - Genetics Molecular cloning 2001-03 Thesis http://psasir.upm.edu.my/id/eprint/9468/ http://psasir.upm.edu.my/id/eprint/9468/1/FSAS_2001_41.pdf text en public masters Universiti Putra Malaysia Lactococcus - Genetics Molecular cloning Faculty of Science and Environmental Studies Sipat, Abdullah English
institution Universiti Putra Malaysia
collection PSAS Institutional Repository
language English
English
advisor Sipat, Abdullah
topic Lactococcus - Genetics
Molecular cloning
spellingShingle Lactococcus - Genetics
Molecular cloning

Ahmat, Nurfilza
Cloning and Expression of Vp2 Gene of Chicken Anemia Vurus Strain Cux-1 in Lactococcus Lactis Mg1363
description In this study, the lactococcal plasmid vector pMG36e, was exploited for the cloning, replication and expression of a viral protein-2 (VP2) gene in the bacterial host, Lactococcus lactis strain MG1363. The VP2 gene (0.65 kb) of chicken anemia virus (CAV) of strain Cuxhaven-l was amplified from viral genomic DNA by polymerase chain reaction (PCR) using the VP2FX and VP2RSai primers (Table 6), cloned into the expression vector pMG36e, and electrotransformed into the L. lactis MG1363 host. Sequencing of the recombinant plasmid pMG36e-VP2 using the Forward and Reverse primers (Table 6), showed 99% homology of the VP2 insert with that of the published VP2 CAY Cux-l sequence. SDS-PAGE and Western blot hybridization of L. lactis (pMG36e-VP2; 4.3 kb), showed no significant protein band of VP2 gene product (24 kDa). However, the gene was transcribed under the P32 promoter of the expression vector pMG36e as shown by a 0.7 kb reverse-transcribed product of the VP2 gene.
format Thesis
qualification_level Master's degree
author Ahmat, Nurfilza
author_facet Ahmat, Nurfilza
author_sort Ahmat, Nurfilza
title Cloning and Expression of Vp2 Gene of Chicken Anemia Vurus Strain Cux-1 in Lactococcus Lactis Mg1363
title_short Cloning and Expression of Vp2 Gene of Chicken Anemia Vurus Strain Cux-1 in Lactococcus Lactis Mg1363
title_full Cloning and Expression of Vp2 Gene of Chicken Anemia Vurus Strain Cux-1 in Lactococcus Lactis Mg1363
title_fullStr Cloning and Expression of Vp2 Gene of Chicken Anemia Vurus Strain Cux-1 in Lactococcus Lactis Mg1363
title_full_unstemmed Cloning and Expression of Vp2 Gene of Chicken Anemia Vurus Strain Cux-1 in Lactococcus Lactis Mg1363
title_sort cloning and expression of vp2 gene of chicken anemia vurus strain cux-1 in lactococcus lactis mg1363
granting_institution Universiti Putra Malaysia
granting_department Faculty of Science and Environmental Studies
publishDate 2001
url http://psasir.upm.edu.my/id/eprint/9468/1/FSAS_2001_41.pdf
_version_ 1794018843361804288

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