Nucleocapsid Protein of Newcastle Disease Virus as an Antigenic Carrier

Newcastle disease virus (NDV) is an economically important avian virus that causes loss to the poultry industry. It has a wide host range infecting 27 of the 50 orders of birds. Generally, the virus consists of six structural proteins: nucleocapsid (NP), phosphoprotein (P), matrix (M), fusion (F)...

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Main Author:	Rabu, Amir
Format:	Thesis
Language:	English English
Published:	2002
Subjects:	Newcastle disease virus Cytoskeletal proteins
Online Access:	http://psasir.upm.edu.my/id/eprint/9491/1/FSAS_2002_31_A.pdf
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id	my-upm-ir.9491
record_format	uketd_dc
spelling	my-upm-ir.94912013-10-01T01:28:44Z Nucleocapsid Protein of Newcastle Disease Virus as an Antigenic Carrier 2002-07 Rabu, Amir Newcastle disease virus (NDV) is an economically important avian virus that causes loss to the poultry industry. It has a wide host range infecting 27 of the 50 orders of birds. Generally, the virus consists of six structural proteins: nucleocapsid (NP), phosphoprotein (P), matrix (M), fusion (F), haemagglutinin-neuramidase (HN) and large (L). The NP protein resembles the classical herringbone morphology when observed under electron microscope. However, the morphology changed into individual ring-like particles when the myc epitope and six histidine residues were fused to the C-terminal end of the protein. Further investigation showed that the C-terminus of this protein derivative is exposed on the surface of the ring-like particles. In this project, several chimeric proteins have been constructed in which the antigenic regions of the HN or F protein of NDV strain AF2240, myc epitope and six histidine residues were linked to the C-tenninus of the NP protein. The chimeric proteins were expressed efficiently in Escherichia coli as detected by Western blot analysis. Electron microscopic analysis on these proteins revealed that they assembled into ring-like particles. These chimeric NP proteins exhibited antigenicity of the myc epitope suggesting that the foreign sequences were exposed on the surface of the particles. Chickens vaccinated with the chimeric particles exhibited an immune response against NDY. However, no protection was observed when the vaccinated chickens were challenged by the virus. Newcastle disease virus Cytoskeletal proteins 2002-07 Thesis http://psasir.upm.edu.my/id/eprint/9491/ http://psasir.upm.edu.my/id/eprint/9491/1/FSAS_2002_31_A.pdf application/pdf en public masters Universiti Putra Malaysia Newcastle disease virus Cytoskeletal proteins Faculty of Science and Environmental Studies English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Newcastle disease virus Cytoskeletal proteins
spellingShingle	Newcastle disease virus Cytoskeletal proteins Rabu, Amir Nucleocapsid Protein of Newcastle Disease Virus as an Antigenic Carrier
description	Newcastle disease virus (NDV) is an economically important avian virus that causes loss to the poultry industry. It has a wide host range infecting 27 of the 50 orders of birds. Generally, the virus consists of six structural proteins: nucleocapsid (NP), phosphoprotein (P), matrix (M), fusion (F), haemagglutinin-neuramidase (HN) and large (L). The NP protein resembles the classical herringbone morphology when observed under electron microscope. However, the morphology changed into individual ring-like particles when the myc epitope and six histidine residues were fused to the C-terminal end of the protein. Further investigation showed that the C-terminus of this protein derivative is exposed on the surface of the ring-like particles. In this project, several chimeric proteins have been constructed in which the antigenic regions of the HN or F protein of NDV strain AF2240, myc epitope and six histidine residues were linked to the C-tenninus of the NP protein. The chimeric proteins were expressed efficiently in Escherichia coli as detected by Western blot analysis. Electron microscopic analysis on these proteins revealed that they assembled into ring-like particles. These chimeric NP proteins exhibited antigenicity of the myc epitope suggesting that the foreign sequences were exposed on the surface of the particles. Chickens vaccinated with the chimeric particles exhibited an immune response against NDY. However, no protection was observed when the vaccinated chickens were challenged by the virus.
format	Thesis
qualification_level	Master's degree
author	Rabu, Amir
author_facet	Rabu, Amir
author_sort	Rabu, Amir
title	Nucleocapsid Protein of Newcastle Disease Virus as an Antigenic Carrier
title_short	Nucleocapsid Protein of Newcastle Disease Virus as an Antigenic Carrier
title_full	Nucleocapsid Protein of Newcastle Disease Virus as an Antigenic Carrier
title_fullStr	Nucleocapsid Protein of Newcastle Disease Virus as an Antigenic Carrier
title_full_unstemmed	Nucleocapsid Protein of Newcastle Disease Virus as an Antigenic Carrier
title_sort	nucleocapsid protein of newcastle disease virus as an antigenic carrier
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Science and Environmental Studies
publishDate	2002
url	http://psasir.upm.edu.my/id/eprint/9491/1/FSAS_2002_31_A.pdf
_version_	1747810965397700608

Nucleocapsid Protein of Newcastle Disease Virus as an Antigenic Carrier

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