The Nucleotide Sequence of the Matrix (M) Protein Gene of Newcastle Disease Virus (NDV) Strain AF 2240

The complete nucleotide sequence of the matrix (M) protein gene of the local Newcastle disease virus (NDV) strain AF 2240 was determined. Based on consensus primers, several segments of the M gene were reverse transcribed and amplified by the polymerase chain reaction. The RT-PCR products were c...

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Main Author:	Jemain, Siti Fathimah Putery
Format:	Thesis
Language:	English English
Published:	1999
Subjects:	Newcastle disease virus Nucleotide sequence
Online Access:	http://psasir.upm.edu.my/id/eprint/9494/1/FSAS_1999_31_A.pdf
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id	my-upm-ir.9494
record_format	uketd_dc
spelling	my-upm-ir.94942013-09-25T08:26:23Z The Nucleotide Sequence of the Matrix (M) Protein Gene of Newcastle Disease Virus (NDV) Strain AF 2240 1999-03 Jemain, Siti Fathimah Putery The complete nucleotide sequence of the matrix (M) protein gene of the local Newcastle disease virus (NDV) strain AF 2240 was determined. Based on consensus primers, several segments of the M gene were reverse transcribed and amplified by the polymerase chain reaction. The RT-PCR products were cloned into a plasmid vector pCR™2.1. The DNA inserts in the clones were then cycle-sequenced. The start and polyadenylation signals have been identified. Assuming that the M gene starts and terminates at these sequences, the M gene is 1223 nucleotides long and encodes an open reading frame of 364 amino acids, corresponding to a polypeptide calculated molecular weight of 40 kDa. The M protein, is both hydrophobic and basic. Phylogenetic analysis shows that the strain has a close relationship with the North American Fontana strain. The M protein amino acid sequence also shows similarities with members of the Rub ulavirus genus such as the Simian virus 5, the human parainfluenza virus type 4 and the mumps virus. Similarities of the amino acid sequence also exist between the local strain and other members of the Paramyxovirus and Morbillivirus genus. Newcastle disease virus Nucleotide sequence 1999-03 Thesis http://psasir.upm.edu.my/id/eprint/9494/ http://psasir.upm.edu.my/id/eprint/9494/1/FSAS_1999_31_A.pdf application/pdf en public masters Universiti Putra Malaysia Newcastle disease virus Nucleotide sequence Faculty of Science and Environmental Studies English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Newcastle disease virus Nucleotide sequence
spellingShingle	Newcastle disease virus Nucleotide sequence Jemain, Siti Fathimah Putery The Nucleotide Sequence of the Matrix (M) Protein Gene of Newcastle Disease Virus (NDV) Strain AF 2240
description	The complete nucleotide sequence of the matrix (M) protein gene of the local Newcastle disease virus (NDV) strain AF 2240 was determined. Based on consensus primers, several segments of the M gene were reverse transcribed and amplified by the polymerase chain reaction. The RT-PCR products were cloned into a plasmid vector pCR™2.1. The DNA inserts in the clones were then cycle-sequenced. The start and polyadenylation signals have been identified. Assuming that the M gene starts and terminates at these sequences, the M gene is 1223 nucleotides long and encodes an open reading frame of 364 amino acids, corresponding to a polypeptide calculated molecular weight of 40 kDa. The M protein, is both hydrophobic and basic. Phylogenetic analysis shows that the strain has a close relationship with the North American Fontana strain. The M protein amino acid sequence also shows similarities with members of the Rub ulavirus genus such as the Simian virus 5, the human parainfluenza virus type 4 and the mumps virus. Similarities of the amino acid sequence also exist between the local strain and other members of the Paramyxovirus and Morbillivirus genus.
format	Thesis
qualification_level	Master's degree
author	Jemain, Siti Fathimah Putery
author_facet	Jemain, Siti Fathimah Putery
author_sort	Jemain, Siti Fathimah Putery
title	The Nucleotide Sequence of the Matrix (M) Protein Gene of Newcastle Disease Virus (NDV) Strain AF 2240
title_short	The Nucleotide Sequence of the Matrix (M) Protein Gene of Newcastle Disease Virus (NDV) Strain AF 2240
title_full	The Nucleotide Sequence of the Matrix (M) Protein Gene of Newcastle Disease Virus (NDV) Strain AF 2240
title_fullStr	The Nucleotide Sequence of the Matrix (M) Protein Gene of Newcastle Disease Virus (NDV) Strain AF 2240
title_full_unstemmed	The Nucleotide Sequence of the Matrix (M) Protein Gene of Newcastle Disease Virus (NDV) Strain AF 2240
title_sort	nucleotide sequence of the matrix (m) protein gene of newcastle disease virus (ndv) strain af 2240
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Science and Environmental Studies
publishDate	1999
url	http://psasir.upm.edu.my/id/eprint/9494/1/FSAS_1999_31_A.pdf
_version_	1747810966126460928

The Nucleotide Sequence of the Matrix (M) Protein Gene of Newcastle Disease Virus (NDV) Strain AF 2240

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