Enzymatic Synthesis of Palm Based Amino Acid Esters
Acyllysines were successfully prepared from reactions of free fatty acids (FFAs) (palmitic acid, PA; oleic acid, OA), triglycerides (TGs) (tripalmitin, TP ; triolein, TO) and palm oil fractions (palm olein, PO; palm kernel olein, PK O) with the unprotected amino acid, L-lysine, using enzymes as b...
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my-upm-ir.95632024-03-08T01:12:13Z Enzymatic Synthesis of Palm Based Amino Acid Esters 2003-04 Soo, Ee Lin Acyllysines were successfully prepared from reactions of free fatty acids (FFAs) (palmitic acid, PA; oleic acid, OA), triglycerides (TGs) (tripalmitin, TP ; triolein, TO) and palm oil fractions (palm olein, PO; palm kernel olein, PK O) with the unprotected amino acid, L-lysine, using enzymes as biocatalyst in an organic solvent system. Such a selective enzymatic route obviates the fastidious regime of substrate activation/protection required in chemical synthesis. Preliminary detection and identification of reaction products were facilitated by thin layer chromatography (TLC) while subsequent quantitative studies on main and interactive effects of parameters governing the reactions were conducted with TLC-photodensitometry. Enzyme screening revealed Lipozyme IM to be the most efficient biocatalyst for the reactions. By varying one-parameter-at-a-time, 70°C was shown to be the optimal temperature, hexane, the most suitable solvent, and, 5:1 (PA:L-lysine), 3:1 (OA:L-lysine), 1:1 (TP:L-lysine), 1:3 (TO:L-lysine), 1:1 (pO:L-lysine) and 1:I(PKO:L-lysine) the respective optimal substrate molar ratios. To reach equilibrium, reactions of FF As and TGs with L-lysine required 4 days while those of the more complex PO and PKO required 6 days. Higher biocatalyst amounts improved all reactions (except that of TP with L-lysine), as did higher amounts of molecular sieves added to reactions ofOA and TO with L-lysine. Enzymes - Synthesis Amino acids - Synthesis 2003-04 Thesis http://psasir.upm.edu.my/id/eprint/9563/ http://psasir.upm.edu.my/id/eprint/9563/1/FSAS_2003_23.pdf text en public doctoral Universiti Putra Malaysia Enzymes - Synthesis Amino acids - Synthesis Faculty of Science and Environmental Studies Salleh, Abu Bakar English |
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Universiti Putra Malaysia |
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PSAS Institutional Repository |
language |
English English |
advisor |
Salleh, Abu Bakar |
topic |
Enzymes - Synthesis Amino acids - Synthesis |
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Enzymes - Synthesis Amino acids - Synthesis Soo, Ee Lin Enzymatic Synthesis of Palm Based Amino Acid Esters |
description |
Acyllysines were successfully prepared from reactions of free fatty acids (FFAs)
(palmitic acid, PA; oleic acid, OA), triglycerides (TGs) (tripalmitin, TP ; triolein, TO)
and palm oil fractions (palm olein, PO; palm kernel olein, PK O) with the unprotected
amino acid, L-lysine, using enzymes as biocatalyst in an organic solvent system. Such a
selective enzymatic route obviates the fastidious regime of substrate
activation/protection required in chemical synthesis. Preliminary detection and
identification of reaction products were facilitated by thin layer chromatography (TLC)
while subsequent quantitative studies on main and interactive effects of parameters
governing the reactions were conducted with TLC-photodensitometry.
Enzyme screening revealed Lipozyme IM to be the most efficient biocatalyst for the
reactions. By varying one-parameter-at-a-time, 70°C was shown to be the optimal
temperature, hexane, the most suitable solvent, and, 5:1 (PA:L-lysine), 3:1 (OA:L-lysine), 1:1 (TP:L-lysine), 1:3 (TO:L-lysine), 1:1 (pO:L-lysine) and 1:I(PKO:L-lysine)
the respective optimal substrate molar ratios. To reach equilibrium, reactions of FF As
and TGs with L-lysine required 4 days while those of the more complex PO and PKO
required 6 days. Higher biocatalyst amounts improved all reactions (except that of TP
with L-lysine), as did higher amounts of molecular sieves added to reactions ofOA and
TO with L-lysine. |
format |
Thesis |
qualification_level |
Doctorate |
author |
Soo, Ee Lin |
author_facet |
Soo, Ee Lin |
author_sort |
Soo, Ee Lin |
title |
Enzymatic Synthesis of Palm Based Amino Acid Esters |
title_short |
Enzymatic Synthesis of Palm Based Amino Acid Esters |
title_full |
Enzymatic Synthesis of Palm Based Amino Acid Esters |
title_fullStr |
Enzymatic Synthesis of Palm Based Amino Acid Esters |
title_full_unstemmed |
Enzymatic Synthesis of Palm Based Amino Acid Esters |
title_sort |
enzymatic synthesis of palm based amino acid esters |
granting_institution |
Universiti Putra Malaysia |
granting_department |
Faculty of Science and Environmental Studies |
publishDate |
2003 |
url |
http://psasir.upm.edu.my/id/eprint/9563/1/FSAS_2003_23.pdf |
_version_ |
1794018848950714368 |