Interrelations of pleasure deficiency, dopamine high secretion and overconsumption of saccharin on sprague dawley rats

Emotional eating is an overeating, for compensating emotional deficiency or for relieving negative emotions that is activated by dopamine. Dopamine neurons are known for their strong responses to rewards like foods and their critical role in positive motivation. Emotional hunger elicits overeating....

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Main Author:	Zakaria, Toumi
Format:	Thesis
Language:	English
Published:	2020
Subjects:	Saccharin - analysis Dopamine Hyperphagia - pathology
Online Access:	http://psasir.upm.edu.my/id/eprint/97765/1/FPSK%28m%29%202021%2031%20-%20IR.1.pdf
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spelling	my-upm-ir.977652022-11-03T04:28:56Z Interrelations of pleasure deficiency, dopamine high secretion and overconsumption of saccharin on sprague dawley rats 2020-06 Zakaria, Toumi Emotional eating is an overeating, for compensating emotional deficiency or for relieving negative emotions that is activated by dopamine. Dopamine neurons are known for their strong responses to rewards like foods and their critical role in positive motivation. Emotional hunger elicits overeating. However, a link between emotional deficiency, dopamine activation, and overeating has not been established yet. The goal of this research is to identify the relation between the previous parameters by focusing on pleasure deficiency as a type of emotional deficiency, beta-endorphin as a pleasure neurotransmitter, and overconsumption of saccharin as an eating disorder. ELISA method was used for dopamine measurements during hunger, fullness, before tasting saccharin, and after tasting saccharin in rats’ striatum homogenate, while it was used for Beta-endorphin before and after tasting liquid saccharin in rats’ striatum homogenate, to assess their contributions in this type of emotional eating. Results showed that less dopamine is released in full rats and less beta-endorphin is released before liquid saccharin tasting and vice versa. It is proposed that dopamine is involved in the motivation of food intake, while beta-endorphin is involved in pleasure emotion of saccharin intake. Subsequently, full rats were injected subcutaneously with dopamine agonist (quinpirole) and antagonist (raclopride). Quinpirole increased liquid saccharin intake while raclopride decreased it. Food preference was observed in rats during food deprivation and satiety by using two choices of food, Mazuri high fat diet that contains fats without sweet taste represents the caloric part, and liquid saccharin as non-nutritive sweetener that contains sweet taste without any calories represents the pleasure part. Here, two different choices of food with opposite properties were used to compare between caloric and pleasure needs. All hungry rats chose Mazuri high-fat diet to compensate their caloric needs, while all full rats chose liquid saccharin to compensate their pleasure needs. These results suggest that pleasure deficiency stimulates striatum dopamine secretion, that leads to overconsumption of saccharin even though rats are full, to compensate the deficiency. Saccharin - analysis Dopamine Hyperphagia - pathology 2020-06 Thesis http://psasir.upm.edu.my/id/eprint/97765/ http://psasir.upm.edu.my/id/eprint/97765/1/FPSK%28m%29%202021%2031%20-%20IR.1.pdf text en public masters Universiti Putra Malaysia Saccharin - analysis Dopamine Hyperphagia - pathology Mohd Moklas, Mohamad Aris
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
advisor	Mohd Moklas, Mohamad Aris
topic	Saccharin - analysis Dopamine Hyperphagia - pathology
spellingShingle	Saccharin - analysis Dopamine Hyperphagia - pathology Zakaria, Toumi Interrelations of pleasure deficiency, dopamine high secretion and overconsumption of saccharin on sprague dawley rats
description	Emotional eating is an overeating, for compensating emotional deficiency or for relieving negative emotions that is activated by dopamine. Dopamine neurons are known for their strong responses to rewards like foods and their critical role in positive motivation. Emotional hunger elicits overeating. However, a link between emotional deficiency, dopamine activation, and overeating has not been established yet. The goal of this research is to identify the relation between the previous parameters by focusing on pleasure deficiency as a type of emotional deficiency, beta-endorphin as a pleasure neurotransmitter, and overconsumption of saccharin as an eating disorder. ELISA method was used for dopamine measurements during hunger, fullness, before tasting saccharin, and after tasting saccharin in rats’ striatum homogenate, while it was used for Beta-endorphin before and after tasting liquid saccharin in rats’ striatum homogenate, to assess their contributions in this type of emotional eating. Results showed that less dopamine is released in full rats and less beta-endorphin is released before liquid saccharin tasting and vice versa. It is proposed that dopamine is involved in the motivation of food intake, while beta-endorphin is involved in pleasure emotion of saccharin intake. Subsequently, full rats were injected subcutaneously with dopamine agonist (quinpirole) and antagonist (raclopride). Quinpirole increased liquid saccharin intake while raclopride decreased it. Food preference was observed in rats during food deprivation and satiety by using two choices of food, Mazuri high fat diet that contains fats without sweet taste represents the caloric part, and liquid saccharin as non-nutritive sweetener that contains sweet taste without any calories represents the pleasure part. Here, two different choices of food with opposite properties were used to compare between caloric and pleasure needs. All hungry rats chose Mazuri high-fat diet to compensate their caloric needs, while all full rats chose liquid saccharin to compensate their pleasure needs. These results suggest that pleasure deficiency stimulates striatum dopamine secretion, that leads to overconsumption of saccharin even though rats are full, to compensate the deficiency.
format	Thesis
qualification_level	Master's degree
author	Zakaria, Toumi
author_facet	Zakaria, Toumi
author_sort	Zakaria, Toumi
title	Interrelations of pleasure deficiency, dopamine high secretion and overconsumption of saccharin on sprague dawley rats
title_short	Interrelations of pleasure deficiency, dopamine high secretion and overconsumption of saccharin on sprague dawley rats
title_full	Interrelations of pleasure deficiency, dopamine high secretion and overconsumption of saccharin on sprague dawley rats
title_fullStr	Interrelations of pleasure deficiency, dopamine high secretion and overconsumption of saccharin on sprague dawley rats
title_full_unstemmed	Interrelations of pleasure deficiency, dopamine high secretion and overconsumption of saccharin on sprague dawley rats
title_sort	interrelations of pleasure deficiency, dopamine high secretion and overconsumption of saccharin on sprague dawley rats
granting_institution	Universiti Putra Malaysia
publishDate	2020
url	http://psasir.upm.edu.my/id/eprint/97765/1/FPSK%28m%29%202021%2031%20-%20IR.1.pdf
_version_	1776100273685528576

Interrelations of pleasure deficiency, dopamine high secretion and overconsumption of saccharin on sprague dawley rats

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