Probiotic Characteristics of Bacteriocinogenic Lactobacillus Plantarum Strains Isolated from Malaysian Foods

The usage of antimicrobial drugs, such as antibiotics, as growth enhancer in livestock farming is prohibited in many countries. In recent years, antimicrobial resistance associated with the use of antimicrobial drugs in animals has triggered vast public attention. Thus, recent approach to enhance...

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Main Author: Lim, Yin Sze
Format: Thesis
Language:English
English
Published: 2010
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Online Access:http://psasir.upm.edu.my/id/eprint/9834/1/FBSB_2010_1_A.pdf
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spelling my-upm-ir.98342013-05-27T07:43:56Z Probiotic Characteristics of Bacteriocinogenic Lactobacillus Plantarum Strains Isolated from Malaysian Foods 2010-02 Lim, Yin Sze The usage of antimicrobial drugs, such as antibiotics, as growth enhancer in livestock farming is prohibited in many countries. In recent years, antimicrobial resistance associated with the use of antimicrobial drugs in animals has triggered vast public attention. Thus, recent approach to enhance the growth performance of livestock is through supplementation of probiotic and digestive enzymes. Probiotic are live microorganisms, when consumed in adequate amounts will confer health benefit on the host. Generally recognised as safe status of lactic acid bacteria (LAB) and the increasing demand of probiotic in food and feed industry have driven current attempt to determine the probiotic potential of six bacteriocinogenic Lactobacillus plantarum strains (UL4, TL1, RS5, RI11, RG11 and RG14) isolated from Malaysian foods, through in vitro probiotic characterisation study and in vivo animal trials. In the in vitro study, the antimicrobial potential of Lb. plantarum strains and bacteriocins was carried out, followed by the study to assess the ability of these strains to overcome simulated gastric and small intestinal tracts, deconjugate bile salts (sodium taurocholate, sodium glycocholate, sodium taurodeoxycholate and sodium glycodeoxycholate), assimilate cholesterol and adhere to intestinal epithelial Caco-2 cell line. In vivo animal trials were then carried out to evaluate the effectiveness of Lb. plantarum strains and bacteriocins produced on growth performance, intestinal microflora and histomorphological changes, and alteration of short chain fatty acids and serum cholesterol level of rats. In the in vitro study, Lb. plantarum strains and their bacteriocins had exhibited strong bacteriocidal and bacteriostatic antimicrobial action against Gram-positive and –negative bacteria. Lb. plantarum strains had strongly inhibited the growth of Bacillus cereus B43 and Listeria monocytogenes L55, whereas bacteriocins had inhibited the Pediococcus acidilactici 4-46, Enterococcus faecalis N-103, Enterococcus faecium ATCC 19434, Listeria monocytogenes L55, Bacillus cereus B43, Streptococcus pneumoniae S972 and Staphylococcus aureus S942. Lb. plantarum strains were highly tolerance to the exposure of simulated gastric and small intestinal juices. They were able to deconjugate bile salts and assimilated 56.7 – 100.0% of cholesterol. Lb. plantarum strains adhered strongly to the Caco-2 epithelial cell line and reduced the adherence of L. monocytogenes L55 substantially. Probiotics Bacteriocins Lactobacillus 2010-02 Thesis http://psasir.upm.edu.my/id/eprint/9834/ http://psasir.upm.edu.my/id/eprint/9834/1/FBSB_2010_1_A.pdf application/pdf en public phd doctoral Universiti Putra Malaysia Probiotics Bacteriocins Lactobacillus Faculty Biotechnology and Biomolecular Sciences English
institution Universiti Putra Malaysia
collection PSAS Institutional Repository
language English
English
topic Probiotics
Bacteriocins
Lactobacillus
spellingShingle Probiotics
Bacteriocins
Lactobacillus
Lim, Yin Sze
Probiotic Characteristics of Bacteriocinogenic Lactobacillus Plantarum Strains Isolated from Malaysian Foods
description The usage of antimicrobial drugs, such as antibiotics, as growth enhancer in livestock farming is prohibited in many countries. In recent years, antimicrobial resistance associated with the use of antimicrobial drugs in animals has triggered vast public attention. Thus, recent approach to enhance the growth performance of livestock is through supplementation of probiotic and digestive enzymes. Probiotic are live microorganisms, when consumed in adequate amounts will confer health benefit on the host. Generally recognised as safe status of lactic acid bacteria (LAB) and the increasing demand of probiotic in food and feed industry have driven current attempt to determine the probiotic potential of six bacteriocinogenic Lactobacillus plantarum strains (UL4, TL1, RS5, RI11, RG11 and RG14) isolated from Malaysian foods, through in vitro probiotic characterisation study and in vivo animal trials. In the in vitro study, the antimicrobial potential of Lb. plantarum strains and bacteriocins was carried out, followed by the study to assess the ability of these strains to overcome simulated gastric and small intestinal tracts, deconjugate bile salts (sodium taurocholate, sodium glycocholate, sodium taurodeoxycholate and sodium glycodeoxycholate), assimilate cholesterol and adhere to intestinal epithelial Caco-2 cell line. In vivo animal trials were then carried out to evaluate the effectiveness of Lb. plantarum strains and bacteriocins produced on growth performance, intestinal microflora and histomorphological changes, and alteration of short chain fatty acids and serum cholesterol level of rats. In the in vitro study, Lb. plantarum strains and their bacteriocins had exhibited strong bacteriocidal and bacteriostatic antimicrobial action against Gram-positive and –negative bacteria. Lb. plantarum strains had strongly inhibited the growth of Bacillus cereus B43 and Listeria monocytogenes L55, whereas bacteriocins had inhibited the Pediococcus acidilactici 4-46, Enterococcus faecalis N-103, Enterococcus faecium ATCC 19434, Listeria monocytogenes L55, Bacillus cereus B43, Streptococcus pneumoniae S972 and Staphylococcus aureus S942. Lb. plantarum strains were highly tolerance to the exposure of simulated gastric and small intestinal juices. They were able to deconjugate bile salts and assimilated 56.7 – 100.0% of cholesterol. Lb. plantarum strains adhered strongly to the Caco-2 epithelial cell line and reduced the adherence of L. monocytogenes L55 substantially.
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Lim, Yin Sze
author_facet Lim, Yin Sze
author_sort Lim, Yin Sze
title Probiotic Characteristics of Bacteriocinogenic Lactobacillus Plantarum Strains Isolated from Malaysian Foods
title_short Probiotic Characteristics of Bacteriocinogenic Lactobacillus Plantarum Strains Isolated from Malaysian Foods
title_full Probiotic Characteristics of Bacteriocinogenic Lactobacillus Plantarum Strains Isolated from Malaysian Foods
title_fullStr Probiotic Characteristics of Bacteriocinogenic Lactobacillus Plantarum Strains Isolated from Malaysian Foods
title_full_unstemmed Probiotic Characteristics of Bacteriocinogenic Lactobacillus Plantarum Strains Isolated from Malaysian Foods
title_sort probiotic characteristics of bacteriocinogenic lactobacillus plantarum strains isolated from malaysian foods
granting_institution Universiti Putra Malaysia
granting_department Faculty Biotechnology and Biomolecular Sciences
publishDate 2010
url http://psasir.upm.edu.my/id/eprint/9834/1/FBSB_2010_1_A.pdf
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