Bioactive Proteins Produced By Bacillus Subtilis Atcc 21332 And Lactobacillus Plantarum Atcc 8014 Under Antimicrobial Stress

Many reports indicate that antimicrobial agents or antibiotics at low concentrations are able to activate or repress gene transcription process in bacteria. However, there are still limited studies on the potential of natural antimicrobials at low doses acting as an inducer to trigger biological fun...

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Main Author:	Ismatul Nurul Asyikin Binti Ismail
Format:	Thesis
Language:	English
Subjects:	Etil pentanoat antimicrobial agents Allium sativum Bacteria (microorganisms)
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id	my-usim-ddms-12344
record_format	uketd_dc
spelling	my-usim-ddms-123442024-05-29T04:01:51Z Bioactive Proteins Produced By Bacillus Subtilis Atcc 21332 And Lactobacillus Plantarum Atcc 8014 Under Antimicrobial Stress Ismatul Nurul Asyikin Binti Ismail Many reports indicate that antimicrobial agents or antibiotics at low concentrations are able to activate or repress gene transcription process in bacteria. However, there are still limited studies on the potential of natural antimicrobials at low doses acting as an inducer to trigger biological functions in bacteria. Therefore, the purpose of this study is to determine the potential of natural and synthetic antimicrobial compounds, which are Allium sativum and ethyl pentanoate at sub-minimal inhibitory concentration (sub-MIC) in inducing proteins production by Bacillus subtilis ATCC 21332 and Lactobacillus plantarum ATCC 8014. SDS-PAGE analysis showed that two extracellular proteins (with approximate size of 51.36 kD and 9.74 kD) were secreted by B. subtilis ATCC 21332 after being enhanced with A. sativum. Meanwhile, L. plantarum ATCC 8014 produced each three (which are 97.83 kD, 53.56 kD and 46.51 kD in size) as well as two (which are 46.51 kD and 6.91 kD in size) new intracellular proteins after being subjected to A. sativum and ethyl pentanoate subsequently. LC-MS/MS analysis presented that 25 proteins were expressed in mild stress condition and have been catalogued into 12 protein functional classes. The extracellular and intracellular proteins each produced by B. subtilis ATCC 21332 and L. plantarum ATCC 8014 revealed antimicrobial activity against selected Gram-positive and Gram-negative bacteria. Both extracellular and intracellular proteins were relatively heat- resistance at 25-30°C and heat-labile at temperature above 30°C. Hence, B. subtilis ATCC 21332 and L. plantarum ATCC 8014 in stressful condition with the presence of either A. sativum or ethyl pentanoate at sub-MIC level were able to induce the production of bioactive proteins with antimicrobial activity. Universiti Sains Islam Malaysia 2013-07 Thesis en https://oarep.usim.edu.my/handle/123456789/12344 https://oarep.usim.edu.my/bitstreams/da454b27-bc4a-426e-a384-d9cd75c26339/download 8a4605be74aa9ea9d79846c1fba20a33 Etil pentanoat antimicrobial agents Allium sativum Bacteria (microorganisms)
institution	Universiti Sains Islam Malaysia
collection	USIM Institutional Repository
language	English
topic	Etil pentanoat antimicrobial agents Allium sativum Bacteria (microorganisms)
spellingShingle	Etil pentanoat antimicrobial agents Allium sativum Bacteria (microorganisms) Ismatul Nurul Asyikin Binti Ismail Bioactive Proteins Produced By Bacillus Subtilis Atcc 21332 And Lactobacillus Plantarum Atcc 8014 Under Antimicrobial Stress
description	Many reports indicate that antimicrobial agents or antibiotics at low concentrations are able to activate or repress gene transcription process in bacteria. However, there are still limited studies on the potential of natural antimicrobials at low doses acting as an inducer to trigger biological functions in bacteria. Therefore, the purpose of this study is to determine the potential of natural and synthetic antimicrobial compounds, which are Allium sativum and ethyl pentanoate at sub-minimal inhibitory concentration (sub-MIC) in inducing proteins production by Bacillus subtilis ATCC 21332 and Lactobacillus plantarum ATCC 8014. SDS-PAGE analysis showed that two extracellular proteins (with approximate size of 51.36 kD and 9.74 kD) were secreted by B. subtilis ATCC 21332 after being enhanced with A. sativum. Meanwhile, L. plantarum ATCC 8014 produced each three (which are 97.83 kD, 53.56 kD and 46.51 kD in size) as well as two (which are 46.51 kD and 6.91 kD in size) new intracellular proteins after being subjected to A. sativum and ethyl pentanoate subsequently. LC-MS/MS analysis presented that 25 proteins were expressed in mild stress condition and have been catalogued into 12 protein functional classes. The extracellular and intracellular proteins each produced by B. subtilis ATCC 21332 and L. plantarum ATCC 8014 revealed antimicrobial activity against selected Gram-positive and Gram-negative bacteria. Both extracellular and intracellular proteins were relatively heat- resistance at 25-30°C and heat-labile at temperature above 30°C. Hence, B. subtilis ATCC 21332 and L. plantarum ATCC 8014 in stressful condition with the presence of either A. sativum or ethyl pentanoate at sub-MIC level were able to induce the production of bioactive proteins with antimicrobial activity.
format	Thesis
author	Ismatul Nurul Asyikin Binti Ismail
author_facet	Ismatul Nurul Asyikin Binti Ismail
author_sort	Ismatul Nurul Asyikin Binti Ismail
title	Bioactive Proteins Produced By Bacillus Subtilis Atcc 21332 And Lactobacillus Plantarum Atcc 8014 Under Antimicrobial Stress
title_short	Bioactive Proteins Produced By Bacillus Subtilis Atcc 21332 And Lactobacillus Plantarum Atcc 8014 Under Antimicrobial Stress
title_full	Bioactive Proteins Produced By Bacillus Subtilis Atcc 21332 And Lactobacillus Plantarum Atcc 8014 Under Antimicrobial Stress
title_fullStr	Bioactive Proteins Produced By Bacillus Subtilis Atcc 21332 And Lactobacillus Plantarum Atcc 8014 Under Antimicrobial Stress
title_full_unstemmed	Bioactive Proteins Produced By Bacillus Subtilis Atcc 21332 And Lactobacillus Plantarum Atcc 8014 Under Antimicrobial Stress
title_sort	bioactive proteins produced by bacillus subtilis atcc 21332 and lactobacillus plantarum atcc 8014 under antimicrobial stress
granting_institution	Universiti Sains Islam Malaysia
_version_	1812444712173305856

Bioactive Proteins Produced By Bacillus Subtilis Atcc 21332 And Lactobacillus Plantarum Atcc 8014 Under Antimicrobial Stress

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