Effect Of Environmental Growth Conditions On Antimicrobial Protein Produced By Bacillus Subtilis Atcc21332 In The Presence Of Cymbopogon Flexuosus Essential Oil
Exposure of bacteria towards environmental stress factors such as a mild concentration of antimicrobial substances could affect the bacterial growth. The bacteria cells may activate the target genes that are related to stress responses pathway in which will further enhance the secretion of proteins....
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my-usim-ddms-123802024-05-29T04:03:03Z Effect Of Environmental Growth Conditions On Antimicrobial Protein Produced By Bacillus Subtilis Atcc21332 In The Presence Of Cymbopogon Flexuosus Essential Oil Nabilah Ahmad Alhadi Exposure of bacteria towards environmental stress factors such as a mild concentration of antimicrobial substances could affect the bacterial growth. The bacteria cells may activate the target genes that are related to stress responses pathway in which will further enhance the secretion of proteins. However, the bacterial growth and the bioactive proteins synthesis rate are also depended on other factors, including pH and nutrients availability. Therefore, this present study focused on the effect of environmental stress factors (Cymbopogon Flexuosus essential oil) and different growth conditions (pH and nutritional factors) onBacillopeptidase F or bioactive proteins secreted by B. subtilis ATCC21332. The bacterial cells were cultivated in media with 0.01 MIC of C. flexuosus esselitial oil as stress inducer and grown under different pH (pH 6,7 and 8) and 1 % (w/w) of nutritional factors, including carbon (glucose, sucrose and starch), nitrogen (urea, casein, gelatin) and inorganic salts (calcium chloride, sodium nitrate and sodium dihydrogen phosphate) sources by one-variable- at-a-time approach or combination of two and four parameters. SDS-PAGE analysis showed that new extracellular proteins with approximate size of 30.43 kDa, 30.66 kDa, 3 1.92 kDa, 3 1.35 kDa and 30.80 kDa were synthesized by B. subtilis ATCC21332 after being exposed to C. .flex-trostls essential oil and grown in each different media consist of either starch, gelatin, casein, sodium dihydrogen phosphate or calcium chloride. Meanwhile, stressed induced B. subtilis ATCC21332 cultured in two growth parameters (pH 6 and differ nutritional factors) produced Bacillopeptidase F only. However, stressed induced B. subtilis ATCC21332 cultured in four growth parameters (pH 6, starch, calcium chloride and gelatin) secreted 32 kDa new protein. MALDI-TOFITOF analysis on the 32 kDa protein produced was recognized as Vpr and Bacillopeptidase F. The extracellular proteins produced by B. subtilis ATCC21332 revealed antimicrobial activity against Gram positive bacteria (B. cereus and S. epidermidis) compared to Gram-negative bacteria. Hence, B. subtilis ATCC21332 in the presence of C.flexuosus essential oil as stress inducer and cultured in various growth condition by one-variable-at-a-time approach as well as combination of two and four parameters could enhance the secretion of Bacillopeptidase F and new extracellular proteins with antimicrobial activity. Universiti Sains Islam Malaysia 2019-01 Thesis en_US https://oarep.usim.edu.my/handle/123456789/12380 https://oarep.usim.edu.my/bitstreams/2a7cddfc-17b5-4b28-bb7d-3b5617347482/download 8a4605be74aa9ea9d79846c1fba20a33 https://oarep.usim.edu.my/bitstreams/4541daa9-9159-4b57-8120-97666a04bfdc/download 711e8bb434a0e13b25c706cde494ee9e Bacillus subtilis -- Biotechnology Biosurfactants Essences and essential oils |
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Bacillus subtilis -- Biotechnology Biosurfactants Essences and essential oils |
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Bacillus subtilis -- Biotechnology Biosurfactants Essences and essential oils Nabilah Ahmad Alhadi Effect Of Environmental Growth Conditions On Antimicrobial Protein Produced By Bacillus Subtilis Atcc21332 In The Presence Of Cymbopogon Flexuosus Essential Oil |
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Exposure of bacteria towards environmental stress factors such as a mild concentration of antimicrobial substances could affect the bacterial growth. The bacteria cells may activate the target genes that are related to stress responses pathway in which will further enhance the secretion of proteins. However, the bacterial growth and the bioactive proteins synthesis rate are also depended on other factors, including pH and nutrients availability. Therefore, this present study focused on the effect of environmental stress factors (Cymbopogon Flexuosus essential oil) and different growth conditions (pH and nutritional factors) onBacillopeptidase F or bioactive proteins secreted by B. subtilis ATCC21332. The bacterial cells were cultivated in media with 0.01 MIC of C. flexuosus esselitial oil as stress inducer and grown under different pH (pH 6,7 and 8) and 1 % (w/w) of nutritional factors, including carbon (glucose, sucrose and starch), nitrogen (urea, casein, gelatin) and inorganic salts (calcium chloride, sodium nitrate and sodium dihydrogen phosphate) sources by one-variable- at-a-time approach or combination of two and four parameters. SDS-PAGE analysis showed that new extracellular proteins with approximate size of 30.43 kDa, 30.66 kDa, 3 1.92 kDa, 3 1.35 kDa and 30.80 kDa were synthesized by B. subtilis ATCC21332 after being exposed to C. .flex-trostls essential oil and grown in each different media consist of either starch, gelatin, casein, sodium dihydrogen phosphate or calcium chloride. Meanwhile, stressed induced B. subtilis ATCC21332 cultured in two growth parameters (pH 6 and differ nutritional factors) produced Bacillopeptidase F only. However, stressed induced B. subtilis ATCC21332 cultured in four growth parameters (pH 6, starch, calcium chloride and gelatin) secreted 32 kDa new protein. MALDI-TOFITOF analysis on the 32 kDa protein produced was recognized as Vpr and Bacillopeptidase F. The extracellular proteins produced by B. subtilis ATCC21332 revealed antimicrobial activity against Gram positive bacteria (B. cereus and S. epidermidis) compared to Gram-negative bacteria. Hence, B. subtilis ATCC21332 in the presence of C.flexuosus essential oil as stress inducer and cultured in various growth condition by one-variable-at-a-time approach as well as combination of two and four parameters could enhance the secretion of Bacillopeptidase F and new extracellular proteins with antimicrobial activity. |
| format |
Thesis |
| author |
Nabilah Ahmad Alhadi |
| author_facet |
Nabilah Ahmad Alhadi |
| author_sort |
Nabilah Ahmad Alhadi |
| title |
Effect Of Environmental Growth Conditions On Antimicrobial Protein Produced By Bacillus Subtilis Atcc21332 In The Presence Of Cymbopogon Flexuosus Essential Oil |
| title_short |
Effect Of Environmental Growth Conditions On Antimicrobial Protein Produced By Bacillus Subtilis Atcc21332 In The Presence Of Cymbopogon Flexuosus Essential Oil |
| title_full |
Effect Of Environmental Growth Conditions On Antimicrobial Protein Produced By Bacillus Subtilis Atcc21332 In The Presence Of Cymbopogon Flexuosus Essential Oil |
| title_fullStr |
Effect Of Environmental Growth Conditions On Antimicrobial Protein Produced By Bacillus Subtilis Atcc21332 In The Presence Of Cymbopogon Flexuosus Essential Oil |
| title_full_unstemmed |
Effect Of Environmental Growth Conditions On Antimicrobial Protein Produced By Bacillus Subtilis Atcc21332 In The Presence Of Cymbopogon Flexuosus Essential Oil |
| title_sort |
effect of environmental growth conditions on antimicrobial protein produced by bacillus subtilis atcc21332 in the presence of cymbopogon flexuosus essential oil |
| granting_institution |
Universiti Sains Islam Malaysia |
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