Physicochemical Properties And Antimicrobial Activity Of Honey From The Malaysian Stingless Bee Against Selected Pathogenic Bacteria

This study evaluate the physiochemical and antimicrobial activity of stingless bee honey (SBH) or "madu kelulut" produced by five Malaysian stingless bee species (Trigona spp., sample A; Hetrotringona laer'iceap, sample B; Heterotringona thorucica, sample C; Hetero-tringona itama,...

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Main Author:	Salmin Al Mabrouk Ali Al Maghribi
Format:	Thesis
Language:	English
Subjects:	Stingless bee madu kelulut antimicrobial activity Honey > Biotechnology > Analysis
Online Access:	https://oarep.usim.edu.my/bitstreams/43ede5d4-08c4-4b17-b278-7e9c1adab0a7/download https://oarep.usim.edu.my/bitstreams/6ea8cd37-3e2d-4fba-b7dd-71409dd591ba/download https://oarep.usim.edu.my/bitstreams/e385dc9b-82f9-4ddc-9bc3-aa3e33c83161/download https://oarep.usim.edu.my/bitstreams/9276ed12-d59f-46d2-8fbc-e5f1f3df2440/download https://oarep.usim.edu.my/bitstreams/e65b79fc-7fdb-4cd9-bf42-a7481507a19f/download https://oarep.usim.edu.my/bitstreams/1464f439-9231-46ea-b80b-a6e64a5b7873/download https://oarep.usim.edu.my/bitstreams/66fc912e-7e95-4a48-a026-a10581979c65/download https://oarep.usim.edu.my/bitstreams/945ab03f-dbda-4562-b000-5cafc65a9698/download
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spelling	my-usim-ddms-123812024-05-29T19:16:06Z Physicochemical Properties And Antimicrobial Activity Of Honey From The Malaysian Stingless Bee Against Selected Pathogenic Bacteria Salmin Al Mabrouk Ali Al Maghribi This study evaluate the physiochemical and antimicrobial activity of stingless bee honey (SBH) or "madu kelulut" produced by five Malaysian stingless bee species (Trigona spp., sample A; Hetrotringona laer'iceap, sample B; Heterotringona thorucica, sample C; Hetero-tringona itama, sample D; and Heirotringonu terminata, sample E) against Grain negative and positive bacteria namely, Escherichiu coli ATCC 25922, Bacillus subtilis ATCC 21332, Serrutia murcesccrrs ATCC 13880, Staphylococcus aureus ATCC 25923, Salmonella Typhimurium ATCC 13 31 1 and Protcus t, ulgaris ATCC 8427. The moisture content of SBH varied between 29.04 and 37.07%, whereas a%%ra nged between 0.280 for sample A and 0.821 for sample C. Most of the SBH are within the range of yellow shades and amber with total soluble solids (°Brix) of 75.6+ 7 for sample E and 88.6+6 for sample A. The antimicrobial activity was evaluated using well diffusion and microtiter plate assay. The antibacterial activity of SBH varied with bee species and the target bacteria. At 50 % (w/v) of honey sample C and D inhibited all the bacteria; sample A did not inhibit E. coli; Sample C, D and sample E failed to inhibit the growth of S. Typhimurium. The pH of raw SBH samples ranged from pH 2.95+0.2 to 3.57+0 with slight reduction in pH after dilution from 10 to 50%. This study has demonstrated that Malaysian stingless bee honey has antimicrobial properties against the selected microorganism, and the low pH of SBH may be responsible for the antimicrobial activity. Universiti Sains Islam Malaysia 2015-05 Thesis en https://oarep.usim.edu.my/handle/123456789/12381 https://oarep.usim.edu.my/bitstreams/a84adccc-d42b-4dee-a630-716107b9cdd2/download 8a4605be74aa9ea9d79846c1fba20a33 https://oarep.usim.edu.my/bitstreams/43ede5d4-08c4-4b17-b278-7e9c1adab0a7/download 58016b58b9a39bdc4a5c4d21de30b999 https://oarep.usim.edu.my/bitstreams/6ea8cd37-3e2d-4fba-b7dd-71409dd591ba/download 90dba613034f9bc266cbab220bb87e71 https://oarep.usim.edu.my/bitstreams/e385dc9b-82f9-4ddc-9bc3-aa3e33c83161/download 5a0cb0006c6127a5c31a387652b43cb6 https://oarep.usim.edu.my/bitstreams/9276ed12-d59f-46d2-8fbc-e5f1f3df2440/download 68dd1884b718be25d1efa8a7b7ba46b3 https://oarep.usim.edu.my/bitstreams/e65b79fc-7fdb-4cd9-bf42-a7481507a19f/download d6f72762a3fac077ed43844de8a81602 https://oarep.usim.edu.my/bitstreams/1464f439-9231-46ea-b80b-a6e64a5b7873/download edcd24724afd0f9f223d91cbffdf3364 https://oarep.usim.edu.my/bitstreams/66fc912e-7e95-4a48-a026-a10581979c65/download b22cb4b44a9c94b13d15a9b974c25ea5 https://oarep.usim.edu.my/bitstreams/945ab03f-dbda-4562-b000-5cafc65a9698/download 2c9ec61aacc290ec219c3cef1ec8842c https://oarep.usim.edu.my/bitstreams/9e71387c-a1f3-40e7-9468-8ae296156c32/download 7fae483f7fa3e61190c7d1eb0cae8ea5 https://oarep.usim.edu.my/bitstreams/5f62cbbb-4faf-4626-a4a8-93f2d2dd925b/download 57765d72347f8d468f3c3390b5a9a33a https://oarep.usim.edu.my/bitstreams/1ea6e0bd-6055-4382-a0f3-4542fa0d0615/download 70198c3a979729c653388af6ab71eb5a https://oarep.usim.edu.my/bitstreams/cb195367-1e4f-4bcf-a199-f7e07339635a/download b525d174665b51a7b6ed19d0038f9796 https://oarep.usim.edu.my/bitstreams/2205e19a-3254-4385-880a-b1a43b763be1/download 85a403b475260eb8724da8a00c4c66c8 https://oarep.usim.edu.my/bitstreams/b2e1ae7f-850a-4911-b51c-875a89d07f21/download 286269844a65396452be2fd5aec7dc09 https://oarep.usim.edu.my/bitstreams/cb723aa2-2c25-4d33-9dd1-40ae21e74884/download 1b6dc7694aded417e97271e34a712cb8 https://oarep.usim.edu.my/bitstreams/dce94e64-f5b4-4ee2-ae76-24f9fc709691/download d194e04cc7e5b9959afcdf7b50a73568 Stingless bee madu kelulut antimicrobial activity Honey--Biotechnology -- Analysis
institution	Universiti Sains Islam Malaysia
collection	USIM Institutional Repository
language	English
topic	Stingless bee madu kelulut antimicrobial activity Honey--Biotechnology -- Analysis
spellingShingle	Stingless bee madu kelulut antimicrobial activity Honey--Biotechnology -- Analysis Salmin Al Mabrouk Ali Al Maghribi Physicochemical Properties And Antimicrobial Activity Of Honey From The Malaysian Stingless Bee Against Selected Pathogenic Bacteria
description	This study evaluate the physiochemical and antimicrobial activity of stingless bee honey (SBH) or "madu kelulut" produced by five Malaysian stingless bee species (Trigona spp., sample A; Hetrotringona laer'iceap, sample B; Heterotringona thorucica, sample C; Hetero-tringona itama, sample D; and Heirotringonu terminata, sample E) against Grain negative and positive bacteria namely, Escherichiu coli ATCC 25922, Bacillus subtilis ATCC 21332, Serrutia murcesccrrs ATCC 13880, Staphylococcus aureus ATCC 25923, Salmonella Typhimurium ATCC 13 31 1 and Protcus t, ulgaris ATCC 8427. The moisture content of SBH varied between 29.04 and 37.07%, whereas a%%ra nged between 0.280 for sample A and 0.821 for sample C. Most of the SBH are within the range of yellow shades and amber with total soluble solids (°Brix) of 75.6+ 7 for sample E and 88.6+6 for sample A. The antimicrobial activity was evaluated using well diffusion and microtiter plate assay. The antibacterial activity of SBH varied with bee species and the target bacteria. At 50 % (w/v) of honey sample C and D inhibited all the bacteria; sample A did not inhibit E. coli; Sample C, D and sample E failed to inhibit the growth of S. Typhimurium. The pH of raw SBH samples ranged from pH 2.95+0.2 to 3.57+0 with slight reduction in pH after dilution from 10 to 50%. This study has demonstrated that Malaysian stingless bee honey has antimicrobial properties against the selected microorganism, and the low pH of SBH may be responsible for the antimicrobial activity.
format	Thesis
author	Salmin Al Mabrouk Ali Al Maghribi
author_facet	Salmin Al Mabrouk Ali Al Maghribi
author_sort	Salmin Al Mabrouk Ali Al Maghribi
title	Physicochemical Properties And Antimicrobial Activity Of Honey From The Malaysian Stingless Bee Against Selected Pathogenic Bacteria
title_short	Physicochemical Properties And Antimicrobial Activity Of Honey From The Malaysian Stingless Bee Against Selected Pathogenic Bacteria
title_full	Physicochemical Properties And Antimicrobial Activity Of Honey From The Malaysian Stingless Bee Against Selected Pathogenic Bacteria
title_fullStr	Physicochemical Properties And Antimicrobial Activity Of Honey From The Malaysian Stingless Bee Against Selected Pathogenic Bacteria
title_full_unstemmed	Physicochemical Properties And Antimicrobial Activity Of Honey From The Malaysian Stingless Bee Against Selected Pathogenic Bacteria
title_sort	physicochemical properties and antimicrobial activity of honey from the malaysian stingless bee against selected pathogenic bacteria
granting_institution	Universiti Sains Islam Malaysia
url	https://oarep.usim.edu.my/bitstreams/43ede5d4-08c4-4b17-b278-7e9c1adab0a7/download https://oarep.usim.edu.my/bitstreams/6ea8cd37-3e2d-4fba-b7dd-71409dd591ba/download https://oarep.usim.edu.my/bitstreams/e385dc9b-82f9-4ddc-9bc3-aa3e33c83161/download https://oarep.usim.edu.my/bitstreams/9276ed12-d59f-46d2-8fbc-e5f1f3df2440/download https://oarep.usim.edu.my/bitstreams/e65b79fc-7fdb-4cd9-bf42-a7481507a19f/download https://oarep.usim.edu.my/bitstreams/1464f439-9231-46ea-b80b-a6e64a5b7873/download https://oarep.usim.edu.my/bitstreams/66fc912e-7e95-4a48-a026-a10581979c65/download https://oarep.usim.edu.my/bitstreams/945ab03f-dbda-4562-b000-5cafc65a9698/download
_version_	1812444761735299072

Physicochemical Properties And Antimicrobial Activity Of Honey From The Malaysian Stingless Bee Against Selected Pathogenic Bacteria

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