Antimicrobial activity of Indian Oregano (Coleus Aromaticus) against Diabetic wound pathogens

The antimicrobial activity of unsterilized and sterilized ethanolic extract of Coleus aromatic was evaluated by deploying disc diffusion, Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and time kill studies on Escherichia coli, Staphylococcus aureus, Klebsiella pneu...

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Main Author:	Kalesware A/P Muniandy
Format:	Thesis
Language:	English
Subjects:	Anti-infective agents Coleus aromaticus > Analysis > Malaysia Medicinal plants Oregano
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id	my-usim-ddms-12383
record_format	uketd_dc
spelling	my-usim-ddms-123832024-05-29T04:03:17Z Antimicrobial activity of Indian Oregano (Coleus Aromaticus) against Diabetic wound pathogens Kalesware A/P Muniandy The antimicrobial activity of unsterilized and sterilized ethanolic extract of Coleus aromatic was evaluated by deploying disc diffusion, Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and time kill studies on Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia, Pseudomonas aeruginosa and Proteus mirabilis. Zone of inhibition was 17.50 to 27.00 mm for unsterilized extract, 11.00 to 20.00 mm for heat sterilized extract, 6.00 to 12.00 mm for filter sterilized extract, 18.00 to 21.00 mm for ampicillin, 19.50 to 24.00 mm for chloramphenicol and 11.50 to 19.00 mm for streptomycin. MIC is 1.02 to 2.60 mg/ml for unsterilized extract, 2.60 to 5.21 mg/ml for heat sterilized 8.33 to 18.75mg/ml for filter sterilized extract and 0.02 mg/ml for chloramphenicol. MBC ranged from 2.60 to 8.33 mg/ml for unsterilized extract and from 0.03 to 0.04 mg/ml for chloramphenicol. Higher values were noted with heat (4.17 to 20.83 mg/ml). Time kill assay showed log reduction of more than 3 in 2 x MIC unsterilized extract on microorganisms tested. Greater log reduction was observed with heat sterilized extract compared to filter sterilized extract. The extract was also evaluated for its wound healing activity in induced diabetic mice. Excision wound model showed 76.6% of wound area reduction with higher rate of wound epithelialization in the presence of extract whereas the controls just exhibited 55.9% of wound area reduction. Dead space wound model showed increased granulation tissue and its histopathological investigation showed good proliferation of collagen tissue with satisfactory angiogenesis. This study showed the activity of C. aromaticus as adiabetic wound antimicrobial agent. Universiti Sains Islam Malaysia 2015 Thesis en https://oarep.usim.edu.my/handle/123456789/12383 https://oarep.usim.edu.my/bitstreams/bd9942bd-8295-45df-9139-5657ae675f30/download 8a4605be74aa9ea9d79846c1fba20a33 Anti-infective agents Coleus aromaticus -- Analysis -- Malaysia Medicinal plants Oregano
institution	Universiti Sains Islam Malaysia
collection	USIM Institutional Repository
language	English
topic	Anti-infective agents Coleus aromaticus -- Analysis -- Malaysia Medicinal plants Oregano
spellingShingle	Anti-infective agents Coleus aromaticus -- Analysis -- Malaysia Medicinal plants Oregano Kalesware A/P Muniandy Antimicrobial activity of Indian Oregano (Coleus Aromaticus) against Diabetic wound pathogens
description	The antimicrobial activity of unsterilized and sterilized ethanolic extract of Coleus aromatic was evaluated by deploying disc diffusion, Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and time kill studies on Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia, Pseudomonas aeruginosa and Proteus mirabilis. Zone of inhibition was 17.50 to 27.00 mm for unsterilized extract, 11.00 to 20.00 mm for heat sterilized extract, 6.00 to 12.00 mm for filter sterilized extract, 18.00 to 21.00 mm for ampicillin, 19.50 to 24.00 mm for chloramphenicol and 11.50 to 19.00 mm for streptomycin. MIC is 1.02 to 2.60 mg/ml for unsterilized extract, 2.60 to 5.21 mg/ml for heat sterilized 8.33 to 18.75mg/ml for filter sterilized extract and 0.02 mg/ml for chloramphenicol. MBC ranged from 2.60 to 8.33 mg/ml for unsterilized extract and from 0.03 to 0.04 mg/ml for chloramphenicol. Higher values were noted with heat (4.17 to 20.83 mg/ml). Time kill assay showed log reduction of more than 3 in 2 x MIC unsterilized extract on microorganisms tested. Greater log reduction was observed with heat sterilized extract compared to filter sterilized extract. The extract was also evaluated for its wound healing activity in induced diabetic mice. Excision wound model showed 76.6% of wound area reduction with higher rate of wound epithelialization in the presence of extract whereas the controls just exhibited 55.9% of wound area reduction. Dead space wound model showed increased granulation tissue and its histopathological investigation showed good proliferation of collagen tissue with satisfactory angiogenesis. This study showed the activity of C. aromaticus as adiabetic wound antimicrobial agent.
format	Thesis
author	Kalesware A/P Muniandy
author_facet	Kalesware A/P Muniandy
author_sort	Kalesware A/P Muniandy
title	Antimicrobial activity of Indian Oregano (Coleus Aromaticus) against Diabetic wound pathogens
title_short	Antimicrobial activity of Indian Oregano (Coleus Aromaticus) against Diabetic wound pathogens
title_full	Antimicrobial activity of Indian Oregano (Coleus Aromaticus) against Diabetic wound pathogens
title_fullStr	Antimicrobial activity of Indian Oregano (Coleus Aromaticus) against Diabetic wound pathogens
title_full_unstemmed	Antimicrobial activity of Indian Oregano (Coleus Aromaticus) against Diabetic wound pathogens
title_sort	antimicrobial activity of indian oregano (coleus aromaticus) against diabetic wound pathogens
granting_institution	Universiti Sains Islam Malaysia
_version_	1812444761984860160

Antimicrobial activity of Indian Oregano (Coleus Aromaticus) against Diabetic wound pathogens

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