Determination of Hydroxycitric Acid (HCA) content in Roselle (Hibiscus sabdariffa L.) through extraction of Potassium Hydroxycitrate and High Performance Liquid Chromatography (HPLC)

Determination Of hydroxycitric Acid (HCA) Content In Roselle (Hibiscus sabdariffa.L.) Through Extraction Of Potassium Hydroxycitrate And High Performance Liquid Chromatography (HPLC). Roselle (Hibiscus sabdariffa L. from family Malvaceace is relatively a new crop in Malaysia. Roselle is rich with vi...

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Main Author:	Halimaton Saadiah Othman
Format:	Thesis
Language:	English
Subjects:	Roselle
Online Access:	https://oarep.usim.edu.my/bitstreams/daa042e1-ddc6-431c-b7b4-8bcbc9f1aa26/download https://oarep.usim.edu.my/bitstreams/91d66b21-ce08-4534-a918-c038bd5d5fe6/download https://oarep.usim.edu.my/bitstreams/e1b42ba1-62b4-4a74-92db-4f68881bba05/download https://oarep.usim.edu.my/bitstreams/d8846f14-cb23-469d-9b1f-8639598047f1/download https://oarep.usim.edu.my/bitstreams/feb33368-09d6-4e55-a0b2-ddb13da19471/download https://oarep.usim.edu.my/bitstreams/a9ad87fd-5642-40be-93a4-7899d0b12f6b/download https://oarep.usim.edu.my/bitstreams/0f0ea071-1300-46df-a4b6-b0ee3458010e/download
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id	my-usim-ddms-12441
record_format	uketd_dc
institution	Universiti Sains Islam Malaysia
collection	USIM Institutional Repository
language	English
topic	Roselle
spellingShingle	Roselle Halimaton Saadiah Othman Determination of Hydroxycitric Acid (HCA) content in Roselle (Hibiscus sabdariffa L.) through extraction of Potassium Hydroxycitrate and High Performance Liquid Chromatography (HPLC)
description	Determination Of hydroxycitric Acid (HCA) Content In Roselle (Hibiscus sabdariffa.L.) Through Extraction Of Potassium Hydroxycitrate And High Performance Liquid Chromatography (HPLC). Roselle (Hibiscus sabdariffa L. from family Malvaceace is relatively a new crop in Malaysia. Roselle is rich with vitamin C and anthocyanins but recent discovery showed that roselle also contains hydroxycitric acid (HCA). This study was conducted to extract HCA in the form of potassium hydroxycitrate and to determine the concentration of potassium hydroxycitrate in roselle accessions through two comparative HCA extraction method i.e the original extraction method and modified extraction method which used activated charcoal. Eleven samples were used in this study which were roselle Accession 2,3,6,12 and 21 and their respective mutants HS0275-30-4-4-1-1-1, HS0275-30-3-3-1-1-1, HS 03100-29-2-1-17-15-1,HS03100-29-7-1-6-3-1, HS1250-18-18-1-1-1-1 and HS1250-1-18-1-1-1-1 while asam keping (Garcinia atroviridis ) and G. cambogia which were known to contain HCA were used as controls. HCA was extracted from dried dried roselle calyces using methanol and HCA extracted was then stabilised in the form of potassium hydroxycitrate, a form that is both stable and biologically active. Potassium hydroxycitrate was comfirmed through HPLC analysis using standard potassium hydroxycitrate. Results showed that the extracts from both extraction methods gave differences in term of colour. Original extraction method gave extracts colours ranged from beige, pale brown and brown. Modified extraction method gave extract colours from white to offwhite. Original extraction method gave Acc. 21 with the highest yield of extract with 17.8%.The extract yield in modified HCA extraction method was slightly decreased due to the elimination of all water soluble compounds by activated charcoal, however showing Acc. 21 and HS03100-29-7-1-6-3-1 both with 6.0%. pH of 5% extract solution from both extraction methods showed pH value ranged around 7.0-8.6 which were all alkaline. Extracts from both extraction methods showed that they were highly soluble in water and aqueous methanol while soluble in hydrochloric acid. The extracts were insoluble in benzene, chloroform and methanol. All their physico-chemical characteristics complies all the characteristics the same to the control used. HPLC results proved that potassium hydroxycitrate were present in all of roselle samples and controls. The retention time of potassium hydroxycitrate was around 4.500 min. For original extraction method, potassium hydroxycitrate concentration was the highest in Acc. 6 with 82.1mg/100g or 0.082%. While in modified extraction method, the highest potassium hydroxycitrate concentratuon was in HS1250-1-18-1-1-1-1 with 42.5mg/100g or 0.043%. This study demonstrates that the roselle in UKM have content of HCA and through appropriate HCA extraction method, the aim for product development of HCA-based product derived from roselle can be proceeded and succeeded.
format	Thesis
author	Halimaton Saadiah Othman
author_facet	Halimaton Saadiah Othman
author_sort	Halimaton Saadiah Othman
title	Determination of Hydroxycitric Acid (HCA) content in Roselle (Hibiscus sabdariffa L.) through extraction of Potassium Hydroxycitrate and High Performance Liquid Chromatography (HPLC)
title_short	Determination of Hydroxycitric Acid (HCA) content in Roselle (Hibiscus sabdariffa L.) through extraction of Potassium Hydroxycitrate and High Performance Liquid Chromatography (HPLC)
title_full	Determination of Hydroxycitric Acid (HCA) content in Roselle (Hibiscus sabdariffa L.) through extraction of Potassium Hydroxycitrate and High Performance Liquid Chromatography (HPLC)
title_fullStr	Determination of Hydroxycitric Acid (HCA) content in Roselle (Hibiscus sabdariffa L.) through extraction of Potassium Hydroxycitrate and High Performance Liquid Chromatography (HPLC)
title_full_unstemmed	Determination of Hydroxycitric Acid (HCA) content in Roselle (Hibiscus sabdariffa L.) through extraction of Potassium Hydroxycitrate and High Performance Liquid Chromatography (HPLC)
title_sort	determination of hydroxycitric acid (hca) content in roselle (hibiscus sabdariffa l.) through extraction of potassium hydroxycitrate and high performance liquid chromatography (hplc)
granting_institution	Universiti Sains Islam Malaysia
url	https://oarep.usim.edu.my/bitstreams/daa042e1-ddc6-431c-b7b4-8bcbc9f1aa26/download https://oarep.usim.edu.my/bitstreams/91d66b21-ce08-4534-a918-c038bd5d5fe6/download https://oarep.usim.edu.my/bitstreams/e1b42ba1-62b4-4a74-92db-4f68881bba05/download https://oarep.usim.edu.my/bitstreams/d8846f14-cb23-469d-9b1f-8639598047f1/download https://oarep.usim.edu.my/bitstreams/feb33368-09d6-4e55-a0b2-ddb13da19471/download https://oarep.usim.edu.my/bitstreams/a9ad87fd-5642-40be-93a4-7899d0b12f6b/download https://oarep.usim.edu.my/bitstreams/0f0ea071-1300-46df-a4b6-b0ee3458010e/download
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spelling	my-usim-ddms-124412024-05-29T18:34:12Z Determination of Hydroxycitric Acid (HCA) content in Roselle (Hibiscus sabdariffa L.) through extraction of Potassium Hydroxycitrate and High Performance Liquid Chromatography (HPLC) Halimaton Saadiah Othman Determination Of hydroxycitric Acid (HCA) Content In Roselle (Hibiscus sabdariffa.L.) Through Extraction Of Potassium Hydroxycitrate And High Performance Liquid Chromatography (HPLC). Roselle (Hibiscus sabdariffa L. from family Malvaceace is relatively a new crop in Malaysia. Roselle is rich with vitamin C and anthocyanins but recent discovery showed that roselle also contains hydroxycitric acid (HCA). This study was conducted to extract HCA in the form of potassium hydroxycitrate and to determine the concentration of potassium hydroxycitrate in roselle accessions through two comparative HCA extraction method i.e the original extraction method and modified extraction method which used activated charcoal. Eleven samples were used in this study which were roselle Accession 2,3,6,12 and 21 and their respective mutants HS0275-30-4-4-1-1-1, HS0275-30-3-3-1-1-1, HS 03100-29-2-1-17-15-1,HS03100-29-7-1-6-3-1, HS1250-18-18-1-1-1-1 and HS1250-1-18-1-1-1-1 while asam keping (Garcinia atroviridis ) and G. cambogia which were known to contain HCA were used as controls. HCA was extracted from dried dried roselle calyces using methanol and HCA extracted was then stabilised in the form of potassium hydroxycitrate, a form that is both stable and biologically active. Potassium hydroxycitrate was comfirmed through HPLC analysis using standard potassium hydroxycitrate. Results showed that the extracts from both extraction methods gave differences in term of colour. Original extraction method gave extracts colours ranged from beige, pale brown and brown. Modified extraction method gave extract colours from white to offwhite. Original extraction method gave Acc. 21 with the highest yield of extract with 17.8%.The extract yield in modified HCA extraction method was slightly decreased due to the elimination of all water soluble compounds by activated charcoal, however showing Acc. 21 and HS03100-29-7-1-6-3-1 both with 6.0%. pH of 5% extract solution from both extraction methods showed pH value ranged around 7.0-8.6 which were all alkaline. Extracts from both extraction methods showed that they were highly soluble in water and aqueous methanol while soluble in hydrochloric acid. The extracts were insoluble in benzene, chloroform and methanol. All their physico-chemical characteristics complies all the characteristics the same to the control used. HPLC results proved that potassium hydroxycitrate were present in all of roselle samples and controls. The retention time of potassium hydroxycitrate was around 4.500 min. For original extraction method, potassium hydroxycitrate concentration was the highest in Acc. 6 with 82.1mg/100g or 0.082%. While in modified extraction method, the highest potassium hydroxycitrate concentratuon was in HS1250-1-18-1-1-1-1 with 42.5mg/100g or 0.043%. This study demonstrates that the roselle in UKM have content of HCA and through appropriate HCA extraction method, the aim for product development of HCA-based product derived from roselle can be proceeded and succeeded. Universiti Sains Islam Malaysia 2011 Thesis en https://oarep.usim.edu.my/handle/123456789/12441 https://oarep.usim.edu.my/bitstreams/499b5547-f6bb-4825-b17f-aeb90a6d78f8/download 8a4605be74aa9ea9d79846c1fba20a33 https://oarep.usim.edu.my/bitstreams/daa042e1-ddc6-431c-b7b4-8bcbc9f1aa26/download 3e7b4cf2aa9adf4f7369d93dc9d6c0ca https://oarep.usim.edu.my/bitstreams/91d66b21-ce08-4534-a918-c038bd5d5fe6/download af30f7eb2f41ee4adc0fc3a448445555 https://oarep.usim.edu.my/bitstreams/e1b42ba1-62b4-4a74-92db-4f68881bba05/download 62fc71ba5c030042c7802a4aeb85483a https://oarep.usim.edu.my/bitstreams/d8846f14-cb23-469d-9b1f-8639598047f1/download 58538cd6db87014b01274e42fcce7a6b https://oarep.usim.edu.my/bitstreams/feb33368-09d6-4e55-a0b2-ddb13da19471/download a41cf5ca5d17f1f658f1a3f8a74a387a https://oarep.usim.edu.my/bitstreams/a9ad87fd-5642-40be-93a4-7899d0b12f6b/download 0a056635b09a1f5136410b6c8d9bf44d https://oarep.usim.edu.my/bitstreams/0f0ea071-1300-46df-a4b6-b0ee3458010e/download 52be13152d3f8882caf0458ca90b6e8a https://oarep.usim.edu.my/bitstreams/0d929429-3e6c-4432-8df5-1a0fe120f7da/download 68b329da9893e34099c7d8ad5cb9c940 https://oarep.usim.edu.my/bitstreams/274fa33c-91f6-4fbe-b605-86311a67e1cc/download a6be035d5336cc50d3996807a0fc1926 https://oarep.usim.edu.my/bitstreams/2ef4f5b8-60f5-46c4-a136-7cd51d967132/download 2228e977ebea8966e27929f43e39cb67 https://oarep.usim.edu.my/bitstreams/eeaa88ad-09ce-490b-aa85-edcfc390b530/download 9d176d1f5f1b1954e72f6a2eb8776b1a https://oarep.usim.edu.my/bitstreams/ae1b6156-a252-498b-ae09-fc51134a82fc/download a0fa1f4054dc8804363acb0c624caf3a https://oarep.usim.edu.my/bitstreams/85280685-bd9c-427d-961d-dc3e34da1074/download 2228e977ebea8966e27929f43e39cb67 https://oarep.usim.edu.my/bitstreams/39b5cbfd-d865-4592-a6b7-bb80273535fc/download 837be1cbe6dd57dacb9fcf2d67a728bc Roselle

Determination of Hydroxycitric Acid (HCA) content in Roselle (Hibiscus sabdariffa L.) through extraction of Potassium Hydroxycitrate and High Performance Liquid Chromatography (HPLC)

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