The Extraction of Clinacanthus Nutans with Three Solvents using the Mixture Design, and its Extracts Anticancer Activity
Clinacanthus nutans (Acanthaceae), known in Malaysia as "Belalai Gajah" is one of the important herbal medicines grown in tropical part of Asia. It has been traditionally used as anti-inflammation, anti-hepatitis and anti-herpes agent. Recent studies have also shown its high medicinal v...
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my-usim-ddms-125912024-05-29T18:46:13Z The Extraction of Clinacanthus Nutans with Three Solvents using the Mixture Design, and its Extracts Anticancer Activity Abdelwahab Bentayeb Clinacanthus nutans (Acanthaceae), known in Malaysia as "Belalai Gajah" is one of the important herbal medicines grown in tropical part of Asia. It has been traditionally used as anti-inflammation, anti-hepatitis and anti-herpes agent. Recent studies have also shown its high medicinal values for the treatment of cancer. Here in this study, the extraction using different solvents (chloroform, methanol and hexane) and their combinations have been used to extract biochemical compounds in Clinacanthus nutans. The compounds have been determined using gas chromatography-mass spectrometry (GS-MS). The potential anticancer effect of Clinacanthus nutans extracts on colon 38 cancer cells using MTT assays have also been evaluated and the The result of GC-MS showed that the extraction using the combination of chloroform and hexane extracted the maximum number of compounds followed by hexane and combination of three solvents. While the other combinations extracted less number of compounds as compared with above combinations. The extract of three solvents mixtures exhibited a better growth inhibition with IC50 6 pg/ml followed by chloroform extract 15 pg/ml and the combination of chloroform and hexane extract with 19 pg/ml. Other extracts were not able to inhibit up to 50 % of the cell growth. This study suggests that phytochemical constituents present in Clinacanthus nutans extract could be as an alternate drug for anti-cancer. However, additional research needs to be done to understand the mechanism of its anticancer compounds. Universiti Sains Islam Malaysia 2015-02 Thesis en https://oarep.usim.edu.my/handle/123456789/12591 https://oarep.usim.edu.my/bitstreams/1f7827c9-a3ee-4cce-8c72-343ed9cb1546/download 8a4605be74aa9ea9d79846c1fba20a33 https://oarep.usim.edu.my/bitstreams/9d3e7bb7-eeae-4a78-b70a-582ac783bab7/download 438c04a6e3603a59582249657ce0094e https://oarep.usim.edu.my/bitstreams/1af72436-51a8-4c52-8760-9bd5fe678a3f/download e1d448aa44bac8136a2c0937b98c7fd9 https://oarep.usim.edu.my/bitstreams/e2f9013d-36f8-4180-a2e3-7d5568b5ad94/download 8f5c58673bbe82933f095d31051c62a9 https://oarep.usim.edu.my/bitstreams/8b941ca2-10ff-443d-b0fa-dd7c00e13830/download 2515197017edacf6a4a159bd339ee550 https://oarep.usim.edu.my/bitstreams/689ef842-ab38-437d-bf34-367dfdedde89/download 86b0033f2e82f624cacd6768ab4d8ff4 https://oarep.usim.edu.my/bitstreams/d3e1ee4b-e775-4c3e-af78-f55f5482767e/download 62983adc3c20c0c04912cc6140e07475 https://oarep.usim.edu.my/bitstreams/11c11135-c395-4718-bf28-00c776b13777/download 86aff601c0775f71e0cfd9d1d4a389e8 https://oarep.usim.edu.my/bitstreams/b77a0440-5b13-43a0-bf7b-38312a0fa83b/download 01e401529e3a0ac5cf89b98fd0c9c0af https://oarep.usim.edu.my/bitstreams/78032614-fddf-4917-bfbd-fee04fae6d2f/download 68b329da9893e34099c7d8ad5cb9c940 https://oarep.usim.edu.my/bitstreams/97862d68-82cf-46df-925a-e3a7a68ceea1/download f6779ea8206579b834ef56bf85d943ae https://oarep.usim.edu.my/bitstreams/2c73fcb6-a260-45ce-9cbc-921e3c1101ee/download 2228e977ebea8966e27929f43e39cb67 https://oarep.usim.edu.my/bitstreams/a5f6871a-34f7-48b8-8aa1-032d93378785/download 36c6f0b2061da514c400c0bc2749b5cf https://oarep.usim.edu.my/bitstreams/cdc35d57-e9ba-4aae-ba37-db81fb1f79b0/download ff4c8ff01d544500ea4bfea43e6108c1 https://oarep.usim.edu.my/bitstreams/8a29a971-0449-4a44-9bcb-c05a6f907240/download 6cccda74febad7705949cb89ee2669dd https://oarep.usim.edu.my/bitstreams/29cc3954-4244-4a0f-847c-9bcc396a7adf/download 68b329da9893e34099c7d8ad5cb9c940 https://oarep.usim.edu.my/bitstreams/416e9260-4b8a-454d-8875-d3df9bcc3deb/download 9e469f9f01cf48ac7499fddb60354d66 Acanthaceae-- Malaysia Clinacanthus Nutans (Acanthaceae) anticancer activity, Belalai Gajah |
| institution |
Universiti Sains Islam Malaysia |
| collection |
USIM Institutional Repository |
| language |
English |
| topic |
Acanthaceae-- Malaysia Clinacanthus Nutans (Acanthaceae) anticancer activity, Belalai Gajah |
| spellingShingle |
Acanthaceae-- Malaysia Clinacanthus Nutans (Acanthaceae) anticancer activity, Belalai Gajah Abdelwahab Bentayeb The Extraction of Clinacanthus Nutans with Three Solvents using the Mixture Design, and its Extracts Anticancer Activity |
| description |
Clinacanthus nutans (Acanthaceae), known in Malaysia as "Belalai Gajah" is one of
the important herbal medicines grown in tropical part of Asia. It has been traditionally
used as anti-inflammation, anti-hepatitis and anti-herpes agent. Recent studies have
also shown its high medicinal values for the treatment of cancer. Here in this study,
the extraction using different solvents (chloroform, methanol and hexane) and their
combinations have been used to extract biochemical compounds in Clinacanthus
nutans. The compounds have been determined using gas chromatography-mass
spectrometry (GS-MS). The potential anticancer effect of Clinacanthus nutans
extracts on colon 38 cancer cells using MTT assays have also been evaluated and the
The result of GC-MS showed that the extraction using the combination of
chloroform and hexane extracted the maximum number of compounds followed by
hexane and combination of three solvents. While the other combinations extracted less
number of compounds as compared with above combinations. The extract of three
solvents mixtures exhibited a better growth inhibition with IC50 6 pg/ml followed by
chloroform extract 15 pg/ml and the combination of chloroform and hexane extract
with 19 pg/ml. Other extracts were not able to inhibit up to 50 % of the cell growth.
This study suggests that phytochemical constituents present in Clinacanthus nutans
extract could be as an alternate drug for anti-cancer. However, additional research
needs to be done to understand the mechanism of its anticancer compounds. |
| format |
Thesis |
| author |
Abdelwahab Bentayeb |
| author_facet |
Abdelwahab Bentayeb |
| author_sort |
Abdelwahab Bentayeb |
| title |
The Extraction of Clinacanthus Nutans with Three Solvents using the Mixture Design, and its Extracts Anticancer Activity |
| title_short |
The Extraction of Clinacanthus Nutans with Three Solvents using the Mixture Design, and its Extracts Anticancer Activity |
| title_full |
The Extraction of Clinacanthus Nutans with Three Solvents using the Mixture Design, and its Extracts Anticancer Activity |
| title_fullStr |
The Extraction of Clinacanthus Nutans with Three Solvents using the Mixture Design, and its Extracts Anticancer Activity |
| title_full_unstemmed |
The Extraction of Clinacanthus Nutans with Three Solvents using the Mixture Design, and its Extracts Anticancer Activity |
| title_sort |
extraction of clinacanthus nutans with three solvents using the mixture design, and its extracts anticancer activity |
| granting_institution |
Universiti Sains Islam Malaysia |
| url |
https://oarep.usim.edu.my/bitstreams/9d3e7bb7-eeae-4a78-b70a-582ac783bab7/download https://oarep.usim.edu.my/bitstreams/1af72436-51a8-4c52-8760-9bd5fe678a3f/download https://oarep.usim.edu.my/bitstreams/e2f9013d-36f8-4180-a2e3-7d5568b5ad94/download https://oarep.usim.edu.my/bitstreams/8b941ca2-10ff-443d-b0fa-dd7c00e13830/download https://oarep.usim.edu.my/bitstreams/689ef842-ab38-437d-bf34-367dfdedde89/download https://oarep.usim.edu.my/bitstreams/d3e1ee4b-e775-4c3e-af78-f55f5482767e/download https://oarep.usim.edu.my/bitstreams/11c11135-c395-4718-bf28-00c776b13777/download https://oarep.usim.edu.my/bitstreams/b77a0440-5b13-43a0-bf7b-38312a0fa83b/download |
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