Transcriptome Analysis of Porphyromonas Gingivalis in Response to Inhibition with Lactobacillus Rhamnosus Atcc 7469 Cell Free Supernatant
Periodontal disease is a chronic disease that is majorly caused by polymicrobial biofilm formation at the periodontal pocket. The important pathogen of this disease is P. gingivalis where it is involved in biofilm formation and virulent activity on the gingival tissue. Probiotics are suggested as...
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| id |
my-usim-ddms-12678 |
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| record_format |
uketd_dc |
| institution |
Universiti Sains Islam Malaysia |
| collection |
USIM Institutional Repository |
| language |
en_US |
| topic |
Periodontal disease, pathogen, Probiotics, antimicrobial and anti-biofilm properties, L rhamnosus, pathogen, P gingivalis Oral Health Periodontal disease--Molecular aspects |
| spellingShingle |
Periodontal disease, pathogen, Probiotics, antimicrobial and anti-biofilm properties, L rhamnosus, pathogen, P gingivalis Oral Health Periodontal disease--Molecular aspects Nurul Szawani Binti Mohd Zubri Transcriptome Analysis of Porphyromonas Gingivalis in Response to Inhibition with Lactobacillus Rhamnosus Atcc 7469 Cell Free Supernatant |
| description |
Periodontal disease is a chronic disease that is majorly caused by polymicrobial
biofilm formation at the periodontal pocket. The important pathogen of this disease is P.
gingivalis where it is involved in biofilm formation and virulent activity on the gingival
tissue. Probiotics are suggested as an adjunct method to overcome this pathogen's
colonization and biofilm formation. The main objective of this study is to investigate the
molecular mechanism of antimicrobial and anti-biofilm properties of L. rhamnosus
ATCC 7469 cell-free supernatant (CFS) against periodontal pathogen, P. gingivalis. To
achieve the objective, disc diffusion assay, minimum inhibitory concentration (MIC) and
minimum bactericidal concentration (MBC) were carried out to determine the
antimicrobial properties while anti-biofilm assay were carried out with various
concentration of L. rhamnosus ATCC 7469 CFS to assess the anti-biofilm activity.To
identify the effect of L. rhamnosus ATCC 7469 on the gene expression of P. gingivalis,
Next Generation Sequencing (NGS) were employed followed by RT-qPCR to validate
the gene expressions.The antimicrobial screening revealed that L. rhamnosus ATCC
7469 CFS was able to inhibit P. gingivalis with the inhibition zone of 10.64 ± 0.44 mm
with the MIC and MBC value of 25% CFS concentration.. In addition, L. rhamnosus
ATCC 7469 CFS able to reduce biofilm formation by 84.94% at the highest
concentration of CFS as observed by the biofilm assay. The molecular investigation by
NGS showed that various genes and pathways related to cell survival, metabolism, and
virulent activity were differentially expressed. Then, the affected gene mfa1, kgp, and rgp
with the addition of one more biofilm related gene, FimA were validated by RT-qPCR
technique. The genes of interest expression were in agreement with the NGS outcomes.
In conclusion, probiotics L. rhamnosus ATCC 7469 CFS possess antimicrobial and antibiofilm
properties against P. gingivalis and the effects of exposure to L. rhamnosus
ATCC 7469 CFS are reflected in the gene expression of P. gingivalis |
| format |
Thesis |
| author |
Nurul Szawani Binti Mohd Zubri |
| author_facet |
Nurul Szawani Binti Mohd Zubri |
| author_sort |
Nurul Szawani Binti Mohd Zubri |
| title |
Transcriptome Analysis of Porphyromonas Gingivalis in Response to Inhibition with Lactobacillus Rhamnosus Atcc 7469 Cell Free Supernatant |
| title_short |
Transcriptome Analysis of Porphyromonas Gingivalis in Response to Inhibition with Lactobacillus Rhamnosus Atcc 7469 Cell Free Supernatant |
| title_full |
Transcriptome Analysis of Porphyromonas Gingivalis in Response to Inhibition with Lactobacillus Rhamnosus Atcc 7469 Cell Free Supernatant |
| title_fullStr |
Transcriptome Analysis of Porphyromonas Gingivalis in Response to Inhibition with Lactobacillus Rhamnosus Atcc 7469 Cell Free Supernatant |
| title_full_unstemmed |
Transcriptome Analysis of Porphyromonas Gingivalis in Response to Inhibition with Lactobacillus Rhamnosus Atcc 7469 Cell Free Supernatant |
| title_sort |
transcriptome analysis of porphyromonas gingivalis in response to inhibition with lactobacillus rhamnosus atcc 7469 cell free supernatant |
| granting_institution |
Universiti Sains Islam Malaysia |
| url |
https://oarep.usim.edu.my/bitstreams/553803eb-53dc-4e2c-b57e-3d66d72b7b4f/download https://oarep.usim.edu.my/bitstreams/47dfab87-6028-49df-9205-3c7d4fec638a/download https://oarep.usim.edu.my/bitstreams/12dcce3a-b875-4793-ab17-d940a43ed74f/download https://oarep.usim.edu.my/bitstreams/06b299ea-6cc5-4716-8353-bfe744d0ebe0/download https://oarep.usim.edu.my/bitstreams/4771d07f-ce4f-4c94-8dac-e3e04c6c892f/download https://oarep.usim.edu.my/bitstreams/e86d2cb0-4467-40b4-ad45-b31fa39637f9/download https://oarep.usim.edu.my/bitstreams/d84c5cf2-8837-453c-a2ed-9adbc50e44e2/download https://oarep.usim.edu.my/bitstreams/85d0ad6e-e06f-4b79-a0bc-57794f8a2b8a/download https://oarep.usim.edu.my/bitstreams/db4c3aa1-4a55-419d-8874-9e6dc835120b/download https://oarep.usim.edu.my/bitstreams/4b41331e-5b52-4d7e-9144-f2d9bb2fd9ba/download |
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1812444724717420544 |
| spelling |
my-usim-ddms-126782024-05-29T18:43:47Z Transcriptome Analysis of Porphyromonas Gingivalis in Response to Inhibition with Lactobacillus Rhamnosus Atcc 7469 Cell Free Supernatant Nurul Szawani Binti Mohd Zubri Periodontal disease is a chronic disease that is majorly caused by polymicrobial biofilm formation at the periodontal pocket. The important pathogen of this disease is P. gingivalis where it is involved in biofilm formation and virulent activity on the gingival tissue. Probiotics are suggested as an adjunct method to overcome this pathogen's colonization and biofilm formation. The main objective of this study is to investigate the molecular mechanism of antimicrobial and anti-biofilm properties of L. rhamnosus ATCC 7469 cell-free supernatant (CFS) against periodontal pathogen, P. gingivalis. To achieve the objective, disc diffusion assay, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were carried out to determine the antimicrobial properties while anti-biofilm assay were carried out with various concentration of L. rhamnosus ATCC 7469 CFS to assess the anti-biofilm activity.To identify the effect of L. rhamnosus ATCC 7469 on the gene expression of P. gingivalis, Next Generation Sequencing (NGS) were employed followed by RT-qPCR to validate the gene expressions.The antimicrobial screening revealed that L. rhamnosus ATCC 7469 CFS was able to inhibit P. gingivalis with the inhibition zone of 10.64 ± 0.44 mm with the MIC and MBC value of 25% CFS concentration.. In addition, L. rhamnosus ATCC 7469 CFS able to reduce biofilm formation by 84.94% at the highest concentration of CFS as observed by the biofilm assay. The molecular investigation by NGS showed that various genes and pathways related to cell survival, metabolism, and virulent activity were differentially expressed. Then, the affected gene mfa1, kgp, and rgp with the addition of one more biofilm related gene, FimA were validated by RT-qPCR technique. The genes of interest expression were in agreement with the NGS outcomes. In conclusion, probiotics L. rhamnosus ATCC 7469 CFS possess antimicrobial and antibiofilm properties against P. gingivalis and the effects of exposure to L. rhamnosus ATCC 7469 CFS are reflected in the gene expression of P. gingivalis Universiti Sains Islam Malaysia 2023-09 Thesis en_US https://oarep.usim.edu.my/handle/123456789/12678 https://oarep.usim.edu.my/bitstreams/553803eb-53dc-4e2c-b57e-3d66d72b7b4f/download 18ecec20c17b39d9375893486d9d27ef https://oarep.usim.edu.my/bitstreams/47dfab87-6028-49df-9205-3c7d4fec638a/download 36aec90cb52a376f77500ab043fce560 https://oarep.usim.edu.my/bitstreams/12dcce3a-b875-4793-ab17-d940a43ed74f/download 3b518e5a0ba4973af63bd5fad7f1daea https://oarep.usim.edu.my/bitstreams/06b299ea-6cc5-4716-8353-bfe744d0ebe0/download da577b72ffe348f5f5553bdb422e3569 https://oarep.usim.edu.my/bitstreams/4771d07f-ce4f-4c94-8dac-e3e04c6c892f/download 2f3cf26506677b8b38c55606fba5becb https://oarep.usim.edu.my/bitstreams/e86d2cb0-4467-40b4-ad45-b31fa39637f9/download 7043c2864c2a5d577e7b0abd0a2b0827 https://oarep.usim.edu.my/bitstreams/d84c5cf2-8837-453c-a2ed-9adbc50e44e2/download 6edd6e20ee8d38cb2a5278bf541b4c71 https://oarep.usim.edu.my/bitstreams/85d0ad6e-e06f-4b79-a0bc-57794f8a2b8a/download 30167c7eab037a9ba9083cc266f40f22 https://oarep.usim.edu.my/bitstreams/db4c3aa1-4a55-419d-8874-9e6dc835120b/download c5de27e82f00ef00144752b27b718712 https://oarep.usim.edu.my/bitstreams/4b41331e-5b52-4d7e-9144-f2d9bb2fd9ba/download 03b02e046d217269e70d8cc2e83529ef https://oarep.usim.edu.my/bitstreams/ece7a922-6cf3-4102-9af6-68ce8ff36853/download 8a4605be74aa9ea9d79846c1fba20a33 https://oarep.usim.edu.my/bitstreams/4634a62b-02bb-4ea3-b32c-1fbd45b7492f/download 68b329da9893e34099c7d8ad5cb9c940 https://oarep.usim.edu.my/bitstreams/ac0bcb88-1200-4ada-9bba-551f77a5c53b/download 4d62c66877bd0e31178a178251dd010b https://oarep.usim.edu.my/bitstreams/76eed507-4939-4f36-9b74-eac0dcded42e/download e67bcd200c4c52231e1a7fcf3f1baee3 https://oarep.usim.edu.my/bitstreams/708adf2c-18e2-4e66-9ce8-d3e20db8b74a/download a1be5952e45f532f4b7161056dce8995 https://oarep.usim.edu.my/bitstreams/61021c6d-edcb-465f-a497-4844b2bde0d7/download b2f8306700af0da51d0f4a0f7343388b https://oarep.usim.edu.my/bitstreams/f216b978-5a00-4485-ae98-eb01b2e723d5/download b01eea455f2e1617a3879c40729a50aa https://oarep.usim.edu.my/bitstreams/ced1acd0-c4ed-460f-8ac8-42a51c599af9/download a2f0e2a58592ff6f4925a8ab2029bf8f https://oarep.usim.edu.my/bitstreams/8249a20c-28eb-4c09-a731-1fa5d69e82a1/download 4ebed7b60957f49888323edd412f34b0 https://oarep.usim.edu.my/bitstreams/b33c9c0a-4561-4ea7-969b-ff39b8f9dca7/download 239b886a53ccf3175224149061723909 https://oarep.usim.edu.my/bitstreams/28304601-d241-4d8e-8545-de93a238a5ea/download 1a5a0e239ca37683654efab14e4ce5ba Periodontal disease, pathogen, Probiotics, antimicrobial and anti-biofilm properties, L. rhamnosus, pathogen, P. gingivalis Oral Health Periodontal disease--Molecular aspects |