Optimization Of Media Compositions And Fermentation Conditions For Enhancement of Surfactin Production By Response Surface Methodology (RSM)
Surfactin is a lipopeptide biosurfactant produced by various strain of Bacillus subtilis. Surfactin showed excellent surface-active properties and prominent biological properties that may be important in various scientific and industrial fields. In this experiment. local isolate of B. subtilis denot...
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Universiti Sains Islam Malaysia |
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| topic |
Biosurfactants Surface active agents Biotechnology |
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Biosurfactants Surface active agents Biotechnology Tengku Ab Rahman bin Tengku Nawawi Optimization Of Media Compositions And Fermentation Conditions For Enhancement of Surfactin Production By Response Surface Methodology (RSM) |
| description |
Surfactin is a lipopeptide biosurfactant produced by various strain of Bacillus subtilis. Surfactin showed excellent surface-active properties and prominent biological properties that may be important in various scientific and industrial fields. In this experiment. local isolate of B. subtilis denoted a B .subtilis 3M was used and cultured into the Cooper’s media. By using shake flask fermentation technique, various parameters of media compositions and fermentation conditions (in Cooper’s media) that affected surfactin and biomass production were optimized using response surface methodology (RSM). Glucose (20-60 g/L). ammonium nitrate (0.03-0.07 M). ferrous sulfate (40-200 uM) and manganese sulfate (0.5-2.5 mM) represented factors for media compositions. On the other hand, fermentation time (48-144 h). inoculum volume (1-20 %) and temperature (20-50 °C) were selected as factors for fermentation conditions. The ranges for each factor ,,were determined by applying one-factor-at-a-time (OFAT) optimization. Henceforth. central composite design (CCD) was employed combining with RSM to optimize both media compositions and fermentation conditions for the production of surfactin and biomass in the media culture. Thusly. the experimental data was fitted to a second-order polynomial equation using multiple regression analysis and analyzed using analysis of variance (/\NOV/\). The 3D response surface plots from the mathematical models were utilized to determine optimum conditions. For media compositions . optimal surfactin production ,was observed of Cooper's media supplemented with 43.-+ g/L glucose. 0.052 M ammonium nitrate. 140 p ii ferrous sulfate and 1.73 mM manganese sulfate. Under these conditions, the maximum predicted yield or surfaetin was 190.8 mg/L. As for fermentation conditions, optimal surfaetin production was observed of Cooper’s media in the conditions of 93 hours of fermentation time. 8.2 % or inoculum volume and 25 °C or temperature. Surfactin yield under these conditions was observed at 249. 7 mg/L. Thus. optimization or both media compositions and fermentation conditions by using RSM managed to enhance surfactin yield to some extent significantly than conventional Cooper's media. |
| format |
Thesis |
| author |
Tengku Ab Rahman bin Tengku Nawawi |
| author_facet |
Tengku Ab Rahman bin Tengku Nawawi |
| author_sort |
Tengku Ab Rahman bin Tengku Nawawi |
| title |
Optimization Of Media Compositions And Fermentation Conditions For Enhancement of Surfactin Production By Response Surface Methodology (RSM) |
| title_short |
Optimization Of Media Compositions And Fermentation Conditions For Enhancement of Surfactin Production By Response Surface Methodology (RSM) |
| title_full |
Optimization Of Media Compositions And Fermentation Conditions For Enhancement of Surfactin Production By Response Surface Methodology (RSM) |
| title_fullStr |
Optimization Of Media Compositions And Fermentation Conditions For Enhancement of Surfactin Production By Response Surface Methodology (RSM) |
| title_full_unstemmed |
Optimization Of Media Compositions And Fermentation Conditions For Enhancement of Surfactin Production By Response Surface Methodology (RSM) |
| title_sort |
optimization of media compositions and fermentation conditions for enhancement of surfactin production by response surface methodology (rsm) |
| granting_institution |
Universiti Sains Islam Malaysia |
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my-usim-ddms-126892024-05-29T19:23:06Z Optimization Of Media Compositions And Fermentation Conditions For Enhancement of Surfactin Production By Response Surface Methodology (RSM) Tengku Ab Rahman bin Tengku Nawawi Surfactin is a lipopeptide biosurfactant produced by various strain of Bacillus subtilis. Surfactin showed excellent surface-active properties and prominent biological properties that may be important in various scientific and industrial fields. In this experiment. local isolate of B. subtilis denoted a B .subtilis 3M was used and cultured into the Cooper’s media. By using shake flask fermentation technique, various parameters of media compositions and fermentation conditions (in Cooper’s media) that affected surfactin and biomass production were optimized using response surface methodology (RSM). Glucose (20-60 g/L). ammonium nitrate (0.03-0.07 M). ferrous sulfate (40-200 uM) and manganese sulfate (0.5-2.5 mM) represented factors for media compositions. On the other hand, fermentation time (48-144 h). inoculum volume (1-20 %) and temperature (20-50 °C) were selected as factors for fermentation conditions. The ranges for each factor ,,were determined by applying one-factor-at-a-time (OFAT) optimization. Henceforth. central composite design (CCD) was employed combining with RSM to optimize both media compositions and fermentation conditions for the production of surfactin and biomass in the media culture. Thusly. the experimental data was fitted to a second-order polynomial equation using multiple regression analysis and analyzed using analysis of variance (/\NOV/\). The 3D response surface plots from the mathematical models were utilized to determine optimum conditions. For media compositions . optimal surfactin production ,was observed of Cooper's media supplemented with 43.-+ g/L glucose. 0.052 M ammonium nitrate. 140 p ii ferrous sulfate and 1.73 mM manganese sulfate. Under these conditions, the maximum predicted yield or surfaetin was 190.8 mg/L. As for fermentation conditions, optimal surfaetin production was observed of Cooper’s media in the conditions of 93 hours of fermentation time. 8.2 % or inoculum volume and 25 °C or temperature. Surfactin yield under these conditions was observed at 249. 7 mg/L. Thus. optimization or both media compositions and fermentation conditions by using RSM managed to enhance surfactin yield to some extent significantly than conventional Cooper's media. Universiti Sains Islam Malaysia 2015-11 Thesis en https://oarep.usim.edu.my/handle/123456789/12689 https://oarep.usim.edu.my/bitstreams/e817f4df-e67e-4e93-b297-6b070fc6481e/download 8a4605be74aa9ea9d79846c1fba20a33 https://oarep.usim.edu.my/bitstreams/4df09c3c-6a7d-4226-bc0a-99a12c282985/download 3e17f4c634964b2108790364b41f222f https://oarep.usim.edu.my/bitstreams/76b61b8d-6a1d-4a9f-a811-e0b3f17966b5/download b577850214e0a7984fe75c1e21ac3bfc https://oarep.usim.edu.my/bitstreams/ec5a71f3-8953-48ae-ad72-c7d30f4a361c/download f0ec5bec9ee8c7404159160fead02898 https://oarep.usim.edu.my/bitstreams/55767aa1-7bd5-497d-ad11-56137c58832f/download 5da087cee36754ffe5997f5236182e21 https://oarep.usim.edu.my/bitstreams/44c1d3b3-e26e-4ce7-8333-7491787b944f/download d39e33ad0e621b5b9ff0688e877d6d83 https://oarep.usim.edu.my/bitstreams/fbe7e694-3eac-4b0b-99f8-bba24852a28a/download 86bdb6dcd2a184e98a8cb68a8a69d336 https://oarep.usim.edu.my/bitstreams/0d826c86-2db4-43f7-a15a-3177e2a39672/download ba28db433accbea4cae940fa031cc6ff https://oarep.usim.edu.my/bitstreams/1a07b6af-ff8c-4d11-92fc-30bc025a0935/download 3e0481cff36dacf672287b7d03f3d09a https://oarep.usim.edu.my/bitstreams/2f5f58d5-2c33-4b81-8b44-e41828a792ce/download d382c49799b7a9089d297190529c2948 https://oarep.usim.edu.my/bitstreams/a7bd15fc-b734-48f4-a541-c3fe5cffabf5/download 57be2bec25d1f14392568797241bfa70 https://oarep.usim.edu.my/bitstreams/c5b47add-8852-48bd-a696-f3681bb3d868/download 6a8aae77460d8c25a4ff7fe19505a22c https://oarep.usim.edu.my/bitstreams/f8aa8eab-b11d-47ca-bcc2-90d9e7c3e24c/download 6544343a42aa03679df9526e23057886 https://oarep.usim.edu.my/bitstreams/56f8d7cb-9b3d-45e7-b51c-879df3a6e759/download e17462633bd63284a1dace528a11ca36 https://oarep.usim.edu.my/bitstreams/757f4d1d-5f5e-4942-b577-c83d120e115a/download ccced13667e2a80cac58a470bda4d32d https://oarep.usim.edu.my/bitstreams/bfa93aa4-1722-492a-a4ab-dcb9232b4d8f/download c2f4a9e6ed2fd613c6f7944029257e37 https://oarep.usim.edu.my/bitstreams/0a0c3b29-aa30-4733-b4ca-51b22e0fb865/download 656e9d50e804d1cfae7f7da2ef69632c Biosurfactants Surface active agents Biotechnology |