Antifungal and Antiadhesion Activities of Lactic Acid Bacteria Isolated from Honey against Candida Species
Candida species are recognized as one of the major cause of hospital-acquired infections.They have become resistance to many antifungal agents, there is a need to develop methods to inhibit the growth of pathogenic Candida spp.This study evaluated the ability of lactic acid bacteria against pathogen...
Saved in:
| id |
my-usim-ddms-12699 |
|---|---|
| record_format |
uketd_dc |
| institution |
Universiti Sains Islam Malaysia |
| collection |
USIM Institutional Repository |
| language |
English |
| topic |
Candida species Lactobacillus plantarum antifungal |
| spellingShingle |
Candida species Lactobacillus plantarum antifungal Bulgasem Y Bulgasem Antifungal and Antiadhesion Activities of Lactic Acid Bacteria Isolated from Honey against Candida Species |
| description |
Candida species are recognized as one of the major cause of hospital-acquired infections.They have become resistance to many antifungal agents, there is a need to develop methods to inhibit the growth of pathogenic Candida spp.This study evaluated the ability of lactic acid bacteria against pathogenic Candida spp.Twenty five isolates of lactic acid bacteria (LAB) were isolated from honey samples from Malaysia,Libya, Saudi Arabia and Yemen, were evaluated for antifungal activity against five strains of pathogenic Candida species namely,C albicans ATCC 1405, C glabrata ATCC2001, C. tropicalis ATCC750,C. parapsilosis ATCC 22019 and C. krusei ATCC6258 using dual agar overlay method and well diffusion method.Four isolates of LAB showed good inhibitory activity against Candida spp. And were identified using API 50CHL nad 16SrDNA as Lactobacillus plantarum HS, Lactobacillus curvatus HH,Pediococcus acidilactici HC and Pediococcus pentosaceus HM. Heating the cell free supernatant (CFS) at 90 and 121°C resulted in growth reduction of most Candida spp. Especially growth of C.glabrata was significantly (p˂0.001) and complete inhibition by CFS of L.curvatus HH and P.pentosaceus HM.The antifungal activity of these CFS was active at Ph 3 to 5,but decreased at Ph 6.CFS of L. plantarum HS and P. pentosaceus HM lost their antifungal activity especially, against C.parapsilosis at PH 7.The antifungal activity of CFS L. plantarum HS was decreased when tested with proteinase K indicating that CFS contained protein-like antifungal compaouns but, the antifungal activity of CFS HC,HH and HM was not destroyed when treated with proteinase K. Treating CFS with RNase destroyed the antifungal activity of HC and HH against all Candida spp.The CFS produced by these LAB inhibited the adhesion of five Candida spp. Biofilm in pre-coating and co-incubation experiments.In pre-coating experiment CFS showed significantly (p˂0.05) higher anti-adhesion activity against C. glabrata and C. albicans by 79.4% and 61.1%, respectively.While in co-incubation experiment CFS had high anti-adhesion activity against all Candida spp.This study demonstrated that the cells and supernatants of L. plantarum HS,P. acidilactici HC, L curvatus HH and P. pentosaceus HM that were isolated from honey could be used to inhibit the growth of Candida spp. And decreased the biofilm formation by these pathogen. |
| format |
Thesis |
| author |
Bulgasem Y Bulgasem |
| author_facet |
Bulgasem Y Bulgasem |
| author_sort |
Bulgasem Y Bulgasem |
| title |
Antifungal and Antiadhesion Activities of Lactic Acid Bacteria Isolated from Honey against Candida Species |
| title_short |
Antifungal and Antiadhesion Activities of Lactic Acid Bacteria Isolated from Honey against Candida Species |
| title_full |
Antifungal and Antiadhesion Activities of Lactic Acid Bacteria Isolated from Honey against Candida Species |
| title_fullStr |
Antifungal and Antiadhesion Activities of Lactic Acid Bacteria Isolated from Honey against Candida Species |
| title_full_unstemmed |
Antifungal and Antiadhesion Activities of Lactic Acid Bacteria Isolated from Honey against Candida Species |
| title_sort |
antifungal and antiadhesion activities of lactic acid bacteria isolated from honey against candida species |
| granting_institution |
Universiti Sains Islam Malaysia |
| url |
https://oarep.usim.edu.my/bitstreams/e2d09c43-8824-4dbc-af00-15dac33a84ae/download https://oarep.usim.edu.my/bitstreams/b0b3639d-207c-48e1-88be-40ca4321c333/download https://oarep.usim.edu.my/bitstreams/d092f014-9b6c-4e56-b3e3-7b08bc9fed1a/download https://oarep.usim.edu.my/bitstreams/294ac96a-e86d-4894-9e2e-d0bb46edd8d8/download https://oarep.usim.edu.my/bitstreams/64c759ba-83f0-4f79-8df6-cb5e91b25b6e/download https://oarep.usim.edu.my/bitstreams/7342ad35-3c22-44d3-8723-0ea055cdd0a1/download https://oarep.usim.edu.my/bitstreams/989f2e4c-cfef-4a40-be7f-215b6d52910d/download https://oarep.usim.edu.my/bitstreams/699b0cfe-d069-4352-9116-34bc2c90c897/download https://oarep.usim.edu.my/bitstreams/f6a6a4f9-2e29-4415-8d1d-e4156eef55d6/download |
| _version_ |
1812444768011026432 |
| spelling |
my-usim-ddms-126992024-05-29T19:16:29Z Antifungal and Antiadhesion Activities of Lactic Acid Bacteria Isolated from Honey against Candida Species Bulgasem Y Bulgasem Candida species are recognized as one of the major cause of hospital-acquired infections.They have become resistance to many antifungal agents, there is a need to develop methods to inhibit the growth of pathogenic Candida spp.This study evaluated the ability of lactic acid bacteria against pathogenic Candida spp.Twenty five isolates of lactic acid bacteria (LAB) were isolated from honey samples from Malaysia,Libya, Saudi Arabia and Yemen, were evaluated for antifungal activity against five strains of pathogenic Candida species namely,C albicans ATCC 1405, C glabrata ATCC2001, C. tropicalis ATCC750,C. parapsilosis ATCC 22019 and C. krusei ATCC6258 using dual agar overlay method and well diffusion method.Four isolates of LAB showed good inhibitory activity against Candida spp. And were identified using API 50CHL nad 16SrDNA as Lactobacillus plantarum HS, Lactobacillus curvatus HH,Pediococcus acidilactici HC and Pediococcus pentosaceus HM. Heating the cell free supernatant (CFS) at 90 and 121°C resulted in growth reduction of most Candida spp. Especially growth of C.glabrata was significantly (p˂0.001) and complete inhibition by CFS of L.curvatus HH and P.pentosaceus HM.The antifungal activity of these CFS was active at Ph 3 to 5,but decreased at Ph 6.CFS of L. plantarum HS and P. pentosaceus HM lost their antifungal activity especially, against C.parapsilosis at PH 7.The antifungal activity of CFS L. plantarum HS was decreased when tested with proteinase K indicating that CFS contained protein-like antifungal compaouns but, the antifungal activity of CFS HC,HH and HM was not destroyed when treated with proteinase K. Treating CFS with RNase destroyed the antifungal activity of HC and HH against all Candida spp.The CFS produced by these LAB inhibited the adhesion of five Candida spp. Biofilm in pre-coating and co-incubation experiments.In pre-coating experiment CFS showed significantly (p˂0.05) higher anti-adhesion activity against C. glabrata and C. albicans by 79.4% and 61.1%, respectively.While in co-incubation experiment CFS had high anti-adhesion activity against all Candida spp.This study demonstrated that the cells and supernatants of L. plantarum HS,P. acidilactici HC, L curvatus HH and P. pentosaceus HM that were isolated from honey could be used to inhibit the growth of Candida spp. And decreased the biofilm formation by these pathogen. Universiti Sains Islam Malaysia 2016-01 Thesis en https://oarep.usim.edu.my/handle/123456789/12699 https://oarep.usim.edu.my/bitstreams/a35ed833-7c90-4345-a5df-52fa1c86b7fa/download 8a4605be74aa9ea9d79846c1fba20a33 https://oarep.usim.edu.my/bitstreams/e2d09c43-8824-4dbc-af00-15dac33a84ae/download c5d97884fd42af0d09d18d1f798ef709 https://oarep.usim.edu.my/bitstreams/b0b3639d-207c-48e1-88be-40ca4321c333/download 5a05caa51ed444e3740cf1167a9d9b49 https://oarep.usim.edu.my/bitstreams/d092f014-9b6c-4e56-b3e3-7b08bc9fed1a/download 3ea1126ea1d7759e2d3e43e485e029a5 https://oarep.usim.edu.my/bitstreams/294ac96a-e86d-4894-9e2e-d0bb46edd8d8/download 035c09578833cb1d7c58a42054e29bbc https://oarep.usim.edu.my/bitstreams/64c759ba-83f0-4f79-8df6-cb5e91b25b6e/download 88b5f4dbd5c1766a14aebc980b3e083a https://oarep.usim.edu.my/bitstreams/7342ad35-3c22-44d3-8723-0ea055cdd0a1/download a124c31f0f72941cc7948cad58bbc7c3 https://oarep.usim.edu.my/bitstreams/989f2e4c-cfef-4a40-be7f-215b6d52910d/download 306d589e9c27dc519e19ef747c42d3bf https://oarep.usim.edu.my/bitstreams/699b0cfe-d069-4352-9116-34bc2c90c897/download e0fd5ca4a02d398892d6164712de1c71 https://oarep.usim.edu.my/bitstreams/f6a6a4f9-2e29-4415-8d1d-e4156eef55d6/download e2995dffbddcdb692f044ca8521c32ac https://oarep.usim.edu.my/bitstreams/872b0cc8-03f1-4136-87d1-b8740731f61c/download 68b329da9893e34099c7d8ad5cb9c940 https://oarep.usim.edu.my/bitstreams/530fefb0-dcfb-4f8d-8280-0c0c23530d1e/download 429079e52f342c49301a02e207bfde06 https://oarep.usim.edu.my/bitstreams/16b410be-6654-408b-848e-25babb28d79e/download ff4c8ff01d544500ea4bfea43e6108c1 https://oarep.usim.edu.my/bitstreams/7b5092d5-b825-43b4-be97-cbeac953c397/download 1bb607118047afc5c385b82385dd931f https://oarep.usim.edu.my/bitstreams/0de6746d-8cf9-4412-9412-1f4f268df880/download 4d2e38c48c39eebf43fd2162ffceedf7 https://oarep.usim.edu.my/bitstreams/91a6252c-5196-4c13-ba75-0bd268179e03/download 24d68299ae13852334a122ea657bbaaa https://oarep.usim.edu.my/bitstreams/7795bac0-9b11-4041-b84a-b535cf8b67bb/download 1bb607118047afc5c385b82385dd931f https://oarep.usim.edu.my/bitstreams/bd5e74f5-f6a6-405f-935b-432da80dbb61/download e1c06d85ae7b8b032bef47e42e4c08f9 https://oarep.usim.edu.my/bitstreams/2a59e5e8-35ea-40b9-8cf6-4a171699e4af/download 9e469f9f01cf48ac7499fddb60354d66 Candida species Lactobacillus plantarum antifungal |