Antibacterial Activity of Acmella Paniculata Extracts Against Oral Bacteria (Streptococcus Mutans)
Acmella paniculata, popularly known as the toothache plant, has been widely used as a traditional medicine to help treat diseases associated with toothache and gum infections. Streptococcus mutans is a common bacterium that can cause dental caries. This study aims to determine the antibacterial a...
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my-usim-ddms-13385 |
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uketd_dc |
| institution |
Universiti Sains Islam Malaysia |
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USIM Institutional Repository |
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en_US |
| topic |
Antibacterial activity Acmella paniculata Streptococcus mutans Antibacterial activity Acmella paniculata Streptococcus mutans |
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Antibacterial activity Acmella paniculata Streptococcus mutans Antibacterial activity Acmella paniculata Streptococcus mutans Nur Syahirah Salehuddin Antibacterial Activity of Acmella Paniculata Extracts Against Oral Bacteria (Streptococcus Mutans) |
| description |
Acmella paniculata, popularly known as the toothache plant, has been widely used as a
traditional medicine to help treat diseases associated with toothache and gum infections.
Streptococcus mutans is a common bacterium that can cause dental caries. This study
aims to determine the antibacterial activity of A. paniculata extracts against S. mutans.
Eight samples of A. paniculata leaves and flowers had been successfully extracted using
n-hexane, dichloromethane, acetone and methanol. All samples were tested for
antibacterial activity using the disc diffusion assay, the minimum inhibitory
concentration (MIC), and the minimum bactericidal concentration (MBC). From the
screening, only n-hexane leaves extract (APLHE), methanol leaves extract (APLME),
n-hexane flower extract (APFHE) and dichloromethane flower extract (APFDE)
exhibited antibacterial activity against S. mutans. Therefore, all four samples were
tested with an antibiofilm and time-kill assay to determine the reduction activity and
time-kill rate against S. mutans. The results showed that flower extracts exhibit more
antibacterial activity against S. mutans when compared with leaves extracts. Thus,
scanning electron microscopy (SEM) and transmission electron microscopy (TEM)
were used to examine the morphology of S. mutans under treatment with APFHE and
APFDE. According to both micrograph observations, the shape of S. mutans changed
from coccus and rounded to elongated and lysed after treatments. Then, by using GCMS,
the major phytochemical compound found in both APFHE and APFDE is fatty
acids (such as hexadecanoic acid, oleic acid, and linoleic acid). Finally, transcriptomic
analysis was performed on S. mutans that had been treated with APFDE. The results
proved that APFDE affected the biosynthesis of peptidoglycan, gene expression,
quorum sensing, citrate cycle and glycolysis pathway of S. mutans. In conclusion, the
antibacterial activity of APFDE disrupted various biological processes and pathways of
S. mutans. |
| format |
Thesis |
| author |
Nur Syahirah Salehuddin |
| author_facet |
Nur Syahirah Salehuddin |
| author_sort |
Nur Syahirah Salehuddin |
| title |
Antibacterial Activity of Acmella Paniculata Extracts Against Oral Bacteria (Streptococcus Mutans) |
| title_short |
Antibacterial Activity of Acmella Paniculata Extracts Against Oral Bacteria (Streptococcus Mutans) |
| title_full |
Antibacterial Activity of Acmella Paniculata Extracts Against Oral Bacteria (Streptococcus Mutans) |
| title_fullStr |
Antibacterial Activity of Acmella Paniculata Extracts Against Oral Bacteria (Streptococcus Mutans) |
| title_full_unstemmed |
Antibacterial Activity of Acmella Paniculata Extracts Against Oral Bacteria (Streptococcus Mutans) |
| title_sort |
antibacterial activity of acmella paniculata extracts against oral bacteria (streptococcus mutans) |
| granting_institution |
Universiti Sains Islam Malaysia |
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my-usim-ddms-133852024-05-29T19:07:35Z Antibacterial Activity of Acmella Paniculata Extracts Against Oral Bacteria (Streptococcus Mutans) Nur Syahirah Salehuddin Acmella paniculata, popularly known as the toothache plant, has been widely used as a traditional medicine to help treat diseases associated with toothache and gum infections. Streptococcus mutans is a common bacterium that can cause dental caries. This study aims to determine the antibacterial activity of A. paniculata extracts against S. mutans. Eight samples of A. paniculata leaves and flowers had been successfully extracted using n-hexane, dichloromethane, acetone and methanol. All samples were tested for antibacterial activity using the disc diffusion assay, the minimum inhibitory concentration (MIC), and the minimum bactericidal concentration (MBC). From the screening, only n-hexane leaves extract (APLHE), methanol leaves extract (APLME), n-hexane flower extract (APFHE) and dichloromethane flower extract (APFDE) exhibited antibacterial activity against S. mutans. Therefore, all four samples were tested with an antibiofilm and time-kill assay to determine the reduction activity and time-kill rate against S. mutans. The results showed that flower extracts exhibit more antibacterial activity against S. mutans when compared with leaves extracts. Thus, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to examine the morphology of S. mutans under treatment with APFHE and APFDE. According to both micrograph observations, the shape of S. mutans changed from coccus and rounded to elongated and lysed after treatments. Then, by using GCMS, the major phytochemical compound found in both APFHE and APFDE is fatty acids (such as hexadecanoic acid, oleic acid, and linoleic acid). Finally, transcriptomic analysis was performed on S. mutans that had been treated with APFDE. The results proved that APFDE affected the biosynthesis of peptidoglycan, gene expression, quorum sensing, citrate cycle and glycolysis pathway of S. mutans. In conclusion, the antibacterial activity of APFDE disrupted various biological processes and pathways of S. mutans. Universiti Sains Islam Malaysia 2023-07 Thesis en_US https://oarep.usim.edu.my/handle/123456789/13385 https://oarep.usim.edu.my/bitstreams/2822209d-7ba8-4c49-806e-2df84cbb85d5/download 8a4605be74aa9ea9d79846c1fba20a33 https://oarep.usim.edu.my/bitstreams/5c2120f3-9a7e-4770-b1e0-7257caaa3bde/download 76c5e98a1eb7a5055dba634e22ec3e71 https://oarep.usim.edu.my/bitstreams/0fd1ffd3-2208-4eea-8417-c5afedc470cf/download d0988d25d5c4872e95c3e318faf24087 https://oarep.usim.edu.my/bitstreams/900fbd8b-24c8-46f5-8c19-fb2521a6dad6/download cd7d8c9c8df587316417bfa46e31e9ec https://oarep.usim.edu.my/bitstreams/fc67175f-8598-4776-a38c-478782581916/download a7d315a6ca78330fba32cd13351e5b28 https://oarep.usim.edu.my/bitstreams/5694fa67-b137-4edc-b954-3cc64b210779/download 3765e95bf4d82c5d9a1ef1a8c2bd89a5 https://oarep.usim.edu.my/bitstreams/884be33a-4ad7-470d-843a-df6f30bdf32b/download 7f92a46fcecbee9f6d022c69c56915ad https://oarep.usim.edu.my/bitstreams/33309a85-e545-4466-bb27-d042582c01d4/download 005e65f4eca550377c6b39d342338d39 https://oarep.usim.edu.my/bitstreams/70f0d3f1-af19-43b0-b7a3-4037a2ac031b/download ec65ee23076189e401aee2f234525d65 https://oarep.usim.edu.my/bitstreams/f604f076-d455-4929-9a94-844accc717e9/download 8037d7a6b34f828ec0e57c9c2b884ac0 https://oarep.usim.edu.my/bitstreams/3a3a6125-4c20-46fd-b747-f0f55b9f1c1a/download 6700a61b271d4895c50bf7d59b1fbb34 https://oarep.usim.edu.my/bitstreams/ddc26347-643e-4215-b370-3be16a201434/download 68b329da9893e34099c7d8ad5cb9c940 https://oarep.usim.edu.my/bitstreams/2bc1ec79-0880-4587-ab48-29328a1ffd73/download 3e07286b2a10c4e016c897cf9d279218 https://oarep.usim.edu.my/bitstreams/3778e1fe-17c5-4b7c-861a-f21ac99fa4f1/download 9da1075786e451d9d11028a4289e726c https://oarep.usim.edu.my/bitstreams/2f2bd4d6-03e2-4d4d-84f9-f257187735a2/download 59ee111cc30ba946a9a8326149bb13b7 https://oarep.usim.edu.my/bitstreams/2ccf9c09-fe5e-4183-88cd-268694957b62/download dd54a2fff0e8d0115e1a725f3831f3de https://oarep.usim.edu.my/bitstreams/ddda24db-93a2-4d7a-a815-a5a8fb6e3a5f/download 3b03f3e0e51c6d94805c5049eb8a1c0d https://oarep.usim.edu.my/bitstreams/00d79ab9-0434-4d88-906f-7194f785c4ef/download 60b1bd3d8cac4da35e926d60aabf4dc6 https://oarep.usim.edu.my/bitstreams/c0017d30-d65c-4f83-aa20-6e07cc00800c/download d4164a614724327fbd2a2e681c816ec1 https://oarep.usim.edu.my/bitstreams/cc15befd-ab0c-4281-b10c-edd4a5c5128c/download 92f251e02eeab978e3a0b2dfdaafa960 https://oarep.usim.edu.my/bitstreams/1d31141b-934e-4902-90d8-7f44c11903f8/download 8c91252e4bebf84553c639e01b8dd35d Antibacterial activity, Acmella paniculata, Streptococcus mutans Materia medica, Dental |